{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T05:20:31Z","timestamp":1787721631854,"version":"build-2784847793"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"NSERC-CREATE\/DFG-IRTG training program \u201cProTECT\u2014Plant Responses To Eliminate Critical Threats\u201d","doi-asserted-by":"publisher","award":["CREATE 509257-18"],"award-info":[{"award-number":["CREATE 509257-18"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2024-04318"],"award-info":[{"award-number":["RGPIN-2024-04318"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"award":["RGPIN-2024-04318"],"award-info":[{"award-number":["RGPIN-2024-04318"]}],"id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Sclerotinia sclerotiorum is a broad-spectrum soilborne fungal pathogen, causing substantial yield losses in crops worldwide. Auxiliary Activity Family 5 (AA5) copper radical oxidases (CROs) are members of the carbohydrate active enzymes (CAZymes), valued for their potential as biocatalysts. Although they were reported to contribute to fungal development and disease progression in some phytopathogenic fungi such as Colletotrichum graminicola, the roles of AA5 enzymes in S. sclerotiorum remain largely unexplored. Here, we systematically investigated S. sclerotiorum AA5 genes through reverse genetics and biochemical approaches. Phylogenetic analysis grouped the S. sclerotiorum AA5 proteins into two subfamilies, including three subfamily 1 (AA5_1) members and one subfamily 2 (AA5_2) member. Enzymatic characterization revealed that one AA5_1 enzyme, SsAA5c, exhibited the highest catalytic specificity towards D-glyceraldehyde compared with methylglyoxal and glycerol. In addition, a predicted peroxidase gene (SsPX1) was identified in tandem with the putative AA5_2 gene, SsAA5d. Phenotypic analysis of gene deletion mutants demonstrated that these genes differentially affect fungal development and virulence. While their exact physiological substrates await identification, our findings highlight important roles of AA5 oxidases and the associated peroxidase in the biology of S. sclerotiorum, providing insights that may contribute to future improved disease management strategies.<\/jats:p>","DOI":"10.3390\/pathogens15090885","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:49:34Z","timestamp":1787582974000},"page":"885","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Copper Radical Oxidases Contribute to Virulence in Sclerotinia sclerotiorum"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9452-8987","authenticated-orcid":false,"given":"Jinyi","family":"Tan","sequence":"first","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"},{"name":"Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6194-2933","authenticated-orcid":false,"given":"Jessica Kalyun","family":"Fong","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"},{"name":"Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0101-862X","authenticated-orcid":false,"given":"Harry","family":"Brumer","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"},{"name":"Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6354-2021","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"},{"name":"Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/07060669409500766","article-title":"Index of plant hosts of Sclerotinia sclerotiorum","volume":"16","author":"Boland","year":"1994","journal-title":"Can. J. Plant Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1364-3703.2005.00316.x","article-title":"Sclerotinia sclerotiorum (Lib.) de Bary: Biology and molecular traits of a cosmopolitan pathogen","volume":"7","author":"Bolton","year":"2006","journal-title":"Mol. Plant Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1111\/mpp.13221","article-title":"The evolutionary and molecular features of the broad-host-range plant pathogen Sclerotinia sclerotiorum","volume":"23","author":"Derbyshire","year":"2022","journal-title":"Mol. Plant Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1094\/PHYTO-06-18-0197-RVW","article-title":"Mechanisms of broad host range necrotrophic pathogenesis in Sclerotinia sclerotiorum","volume":"108","author":"Liang","year":"2018","journal-title":"Phytopathology"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Xia, S., Xu, Y., Hoy, R., Zhang, J., Qin, L., and Li, X. (2020). The notorious soilborne pathogenic fungus Sclerotinia sclerotiorum: An update on genes studied with mutant analysis. Pathogens, 9.","DOI":"10.3390\/pathogens9010027"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1080\/07060661.2018.1516699","article-title":"Partial stem resistance in Brassica napus to highly aggressive and genetically diverse Sclerotinia sclerotiorum isolates from Australia","volume":"40","author":"Derbyshire","year":"2018","journal-title":"Can. J. Plant Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1111\/ppa.12517","article-title":"The control of sclerotinia stem rot on oilseed rape (Brassica napus): Current practices and future opportunities","volume":"65","author":"Derbyshire","year":"2016","journal-title":"Plant Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lehner, M.S., De Paula J\u00fanior, T.J., Del Ponte, E.M., Mizubuti, E.S.G., and Pethybridge, S.J. (2017). Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0173915"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e13423","DOI":"10.1111\/mpp.13423","article-title":"The schizotrophic lifestyle of Sclerotinia sclerotiorum","volume":"25","author":"Shang","year":"2024","journal-title":"Mol. Plant Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1007\/s13213-014-0916-x","article-title":"Morphological development of sclerotia by Sclerotinia sclerotiorum: A view from light and scanning electron microscopy","volume":"65","author":"Larsen","year":"2015","journal-title":"Ann. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/BF02860868","article-title":"The biology of Sclerotinia sclerotiorum, S. trifoliorum, and S. minor with emphasis on specific nomenclature","volume":"46","author":"Willetts","year":"1980","journal-title":"Bot. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.fbr.2007.10.001","article-title":"Sclerotial development in Sclerotinia sclerotiorum: Awakening molecular analysis of a \u201cDormant\u201d structure","volume":"22","author":"Erental","year":"2008","journal-title":"Fungal Biol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1146\/annurev-phyto-102313-045831","article-title":"Plant cell wall-degrading enzymes and their secretion in plant-pathogenic fungi","volume":"52","author":"Kubicek","year":"2014","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/jxb\/erae442","article-title":"Plant cell wall structure and dynamics in plant\u2014Pathogen interactions and pathogen defence","volume":"76","author":"Munzert","year":"2025","journal-title":"J. Exp. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1093\/nar\/gkab1045","article-title":"The carbohydrate-active enzyme database: Functions and literature","volume":"50","author":"Drula","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"The CAZypedia Consortium (2018). Ten years of CAZypedia: A living encyclopedia of carbohydrate-active enzymes. Glycobiology, 28, 3\u20138.","DOI":"10.1093\/glycob\/cwx089"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19966","DOI":"10.1038\/s41598-019-56396-w","article-title":"The host generalist phytopathogenic fungus Sclerotinia sclerotiorum differentially expresses multiple metabolic enzymes on two different plant hosts","volume":"9","author":"Allan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Amselem, J., Cuomo, C.A., van Kan, J.A.L., Viaud, M., Benito, E.P., Couloux, A., Coutinho, P.M., de Vries, R.P., Dyer, P.S., and Fillinger, S. (2011). Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genet., 7.","DOI":"10.1371\/journal.pgen.1002230"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"21855","DOI":"10.1038\/s41598-022-22028-z","article-title":"Draft genome sequencing and secretome profiling of Sclerotinia sclerotiorum revealed effector repertoire diversity and allied broad\u2014Host range necrotrophy","volume":"12","author":"Gupta","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Seifbarghi, S., Borhan, M.H., Wei, Y., Coutu, C., Robinson, S.J., and Hegedus, D.D. (2017). Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus. BMC Genom., 18.","DOI":"10.1186\/s12864-017-3642-5"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1042\/EBC20220124","article-title":"Copper radical oxidases: Galactose oxidase, glyoxal oxidase, and beyond!","volume":"67","author":"Fong","year":"2023","journal-title":"Essays Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100037","DOI":"10.1016\/j.engmic.2022.100037","article-title":"Copper-radical oxidases: A diverse group of biocatalysts with distinct properties and a broad range of biotechnological applications","volume":"2","author":"Koschorreck","year":"2022","journal-title":"Eng. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1754-6834-6-41","article-title":"Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes","volume":"6","author":"Levasseur","year":"2013","journal-title":"Biotechnol. Biofuels"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s11274-017-2254-1","article-title":"Glyoxal oxidases: Their nature and properties","volume":"33","author":"Daou","year":"2017","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.abb.2004.08.034","article-title":"The radical chemistry of galactose oxidase","volume":"433","author":"Whittaker","year":"2005","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1073\/pnas.87.8.2936","article-title":"Glyoxal oxidase of Phanerochaete chrysosporium: Its characterization and activation by lignin peroxidase","volume":"87","author":"Kersten","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s10086-009-1105-6","article-title":"Glyoxal oxidase supplies hydrogen peroxide at hyphal tips and on hyphal wall to manganese peroxidase of white-rot fungus Phanerochaete crassa WD1694","volume":"56","author":"Takano","year":"2010","journal-title":"J. Wood Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"eade9982","DOI":"10.1126\/sciadv.ade9982","article-title":"Tandem metalloenzymes gate plant cell entry by pathogenic fungi","volume":"8","author":"Bissaro","year":"2022","journal-title":"Sci. Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.1016\/j.cell.2025.05.017","article-title":"Phenotypic landscape of an invasive fungal pathogen reveals its unique biology ll Article Phenotypic landscape of an invasive fungal","volume":"188","author":"Boucher","year":"2025","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"103245","DOI":"10.1016\/j.fgb.2019.103245","article-title":"Effects on hyphal morphology and development by the putative copper radical oxidase glx1 in Trichoderma virens suggest a novel role as a cell wall associated enzyme","volume":"131","author":"Crutcher","year":"2019","journal-title":"Fungal Genet. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/s00438-004-1085-6","article-title":"A H2O2 -producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis","volume":"272","author":"Leuthner","year":"2005","journal-title":"Mol. Genet. Genom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"337","DOI":"10.17221\/52\/2023-PPS","article-title":"Identification and functional analysis of glyoxal oxidase gene from rubber tree anthracnose","volume":"59","author":"Xian","year":"2023","journal-title":"Plant Prot. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e00790-26","DOI":"10.1128\/aem.00790-26","article-title":"Exploring the biochemical and biological functions of copper radical oxidases in the vascular wilt phytopathogen Verticillium dahliae","volume":"92","author":"Fong","year":"2026","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8187","DOI":"10.1007\/s00018-021-03981-w","article-title":"A survey of substrate specificity among Auxiliary Activity Family 5 copper radical oxidases","volume":"78","author":"Cleveland","year":"2021","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1094\/MPMI-10-21-0254-R","article-title":"A forward genetic screen in Sclerotinia sclerotiorum revealed the transcriptional regulation of its sclerotial melanization pathway","volume":"35","author":"Xu","year":"2022","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1042\/BCJ20260010","article-title":"Structural enzymology of a Fusarium graminearum aldehyde oxidase reveals a distinct active-site and reactivity versus its paralog galactose oxidase","volume":"483","author":"Fong","year":"2026","journal-title":"Biochem. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4867","DOI":"10.1128\/AEM.00304-16","article-title":"Heterologous Production and Characterization of Two Glyoxal Oxidases from Pycnoporus cinnabarinus","volume":"82","author":"Daou","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s40694-019-0067-8","article-title":"Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives","volume":"6","author":"Daou","year":"2019","journal-title":"Fungal Biol. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kadowaki, A.M.S., De Godoy, M.O., Kumagai, P.S., da Costa-Filho, A.J., Mort, A., Prade, R.A., and Polikarpov, I. (2018). Characterization of a new glyoxal oxidase from the Thermophilic fungus Myceliophthora thermophila M77: Hydrogen peroxide production retained in 5-Hydroxymethylfurfural oxidation. Catalysts, 8.","DOI":"10.3390\/catal8100476"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Bissaro, B., Kodama, S., Hage, H., Ribeaucourt, D., Haon, M., Grisel, S., Simaan, A.J., Beisson, F., Forget, S.M., and Brumer, H. (2021). Unravelling the role of alcohol copper radical oxidases in fungal plant pathogens. Res. Sq.","DOI":"10.21203\/rs.3.rs-493001\/v1"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e0101424","DOI":"10.1128\/aem.01014-24","article-title":"Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases","volume":"90","author":"Fong","year":"2024","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/9\/885\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T04:24:32Z","timestamp":1787718272000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/9\/885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":41,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["pathogens15090885"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15090885","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]}}}