{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:09:48Z","timestamp":1788948588481,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018688","name":"Korea Disease Control and Prevention Agency","doi-asserted-by":"publisher","award":["6332-305-210"],"award-info":[{"award-number":["6332-305-210"]}],"id":[{"id":"10.13039\/100018688","id-type":"DOI","asserted-by":"publisher"}]},{"award":["6332-305-210"],"award-info":[{"award-number":["6332-305-210"]}],"id":[{"id":"https:\/\/ror.org\/04jgeq066","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Tick-borne parasitic diseases pose important threats to veterinary and public health, particularly in countries with livestock-dependent economies such as Kyrgyzstan. However, molecular epidemiological data on tick-borne pathogens in Kyrgyzstan is limited. This study investigated the prevalence, genetic diversity, and host\u2013tick\u2013pathogen associations in Chuy Province, Kyrgyzstan. A total of 494 ticks belonging to 6 genera and 10 species were collected from various animal hosts in eight survey areas in central-northern Chuy Province and analyzed using polymerase chain reaction, Sanger sequencing, and phylogenetic analysis. Of the 494 ticks, 35 (7.1%) were infected, including 15 (3.0%) with Babesia species and 20 (4.0%) with Theileria species. The highest positive rates were observed in Rhipicephalus annulatus (13.8%) and Haemaphysalis punctata (12.4%). Molecular analysis identified 4 Babesia species (B. caballi, B. major, B. bigemina, B. occultans) and 2 Theileria species (T. orientalis, T. ovis). Ticks collected from horses had the highest infection rates and greatest diversity of pathogens. T. orientalis was the most common pathogen. T. ovis was exclusively found in ticks collected from sheep. Phylogenetic analysis confirmed that the isolates were closely related to strains circulating in neighboring Central Asian and Eurasian countries. The findings provide foundational data for developing region-specific vector control measures.<\/jats:p>","DOI":"10.3390\/pathogens15090940","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:25:56Z","timestamp":1788542756000},"page":"940","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of Tick-Borne Parasitic Pathogens in Ticks Collected from Animal Hosts in Chuy Province, Kyrgyzstan"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3573-8868","authenticated-orcid":false,"given":"Hyun Jung","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"},{"name":"Department of Medical Science and Infectious Biology, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Ji Ye","family":"Seo","sequence":"additional","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Jin Seo","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5248-6995","authenticated-orcid":false,"given":"Jung-Won","family":"Ju","sequence":"additional","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4853-4899","authenticated-orcid":false,"given":"Bekbolsun","family":"Aknazarov","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Biotechnology, Kyrgyz National Agrarian University Named After Konstantin Ivanovich Skryabin, Bishkek 720005, Kyrgyzstan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Nurzina","family":"Atabekova","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Biotechnology, Kyrgyz National Agrarian University Named After Konstantin Ivanovich Skryabin, Bishkek 720005, Kyrgyzstan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-579X","authenticated-orcid":false,"given":"Sang-Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Hee-Il","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency (KDCA), Cheongju 28159, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetpar.2011.07.012","article-title":"Vector-borne parasitic zoonoses: Emerging scenarios and new perspectives","volume":"182","author":"Colwell","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hoffman, D., Dreghiciu, I.C., Oprescu, I., Imre, M., Florea, T., Plesko, A., Morariu, S., and Ilie, M.S. (2025). Cattle zoonotic and non-zoonotic tick-borne pathogens in Europe\u2014A retrospective analysis of the past 15 years. Animals, 15.","DOI":"10.3390\/ani15101408"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e004725","DOI":"10.1590\/s1984-29612025062","article-title":"Impact of climate change on the tick-host-pathogen complex: Distribution patterns, disease incidence, and host infestation","volume":"34","author":"Alasmari","year":"2025","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Defaye, B., Moutailler, S., Pasqualini, V., and Quilichini, Y. (2022). Distribution of tick-borne pathogens in domestic animals and their ticks in the countries of the Mediterranean basin between 2000 and 2021: A systematic review. Microorganisms, 10.","DOI":"10.3390\/microorganisms10061236"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ttbdis.2014.03.002","article-title":"Ticks of the Hyalomma marginatum complex transported by migratory birds into Central Europe","volume":"5","author":"Capek","year":"2014","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ijppaw.2012.11.003","article-title":"Natural history of Zoonotic Babesia: Role of wildlife reservoirs","volume":"2","author":"Yabsley","year":"2013","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13071-025-07222-y","article-title":"A systematic review of the occurrence of Hyalomma ticks associated with birds migrating between Africa and the Northern Hemisphere","volume":"19","author":"Zhou","year":"2026","journal-title":"Parasit. Vectors"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/vbz.2016.1955","article-title":"Guidelines for the detection of Babesia and Theileria parasites","volume":"17","author":"Lempereur","year":"2017","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ijppaw.2014.12.006","article-title":"A review of Theileria diagnostics and epidemiology","volume":"4","author":"Mans","year":"2015","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Young, K.M., Corrin, T., Wilhelm, B., Uhland, C., Greig, J., Mascarenhas, M., and Waddell, L.A. (2019). Zoonotic Babesia: A scoping review of the global evidence. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0226781"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s40588-015-0025-z","article-title":"Human babesiosis: Pathogens, prevalence, diagnosis, and treatment","volume":"2","author":"Ord","year":"2015","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.ijpara.2018.11.007","article-title":"Human babesiosis","volume":"49","author":"Krause","year":"2019","journal-title":"Int. J. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ttbdis.2009.11.003","article-title":"Zoonotic babesiosis: Overview of the disease and novel aspects of pathogen identity","volume":"1","author":"Gray","year":"2010","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.pt.2012.07.003","article-title":"Ticks and tick-borne diseases: A One Health perspective","volume":"28","author":"Chomel","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Yang, J., Wang, Y., Zhang, H., Su, Y., Wu, X., Yan, S., and Yang, S. (2022). Impact of socio-economic and environmental factors on livestock production in Kyrgyzstan. Front. Environ. Sci., 10.","DOI":"10.3389\/fenvs.2022.1049187"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hay, J., Yeh, K.B., Dasgupta, D., Shapieva, Z., Omasheva, G., Deryabin, P., Nurmakhanov, T., Ayazbayev, T., Andryushchenko, A., and Zhunushov, A. (2016). Biosurveillance in Central Asia: Successes and challenges of tick-borne disease research in Kazakhstan and Kyrgyzstan. Front. Public Health, 4.","DOI":"10.3389\/fpubh.2016.00004"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Altay, K., Erol, U., Sahin, O.F., Ulucesme, M.C., Aytmirzakizi, A., and Aktas, M. (2024). Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: Phylogenetic analysis, genetic diversity, and prevalence of Theileria equi. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1359974"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"102915","DOI":"10.1016\/j.parint.2024.102915","article-title":"Molecular prevalence and genotypic diversity of Theileria equi and Babesia caballi infecting horses in Kyrgyzstan","volume":"102","author":"Atabek","year":"2024","journal-title":"Parasitol. Int."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Oh, S., Amvongo-Adjia, N., Kim, H.J., Choi, J.H., Chavarria, X., Yi, M.H., Shatta, A., Aknazarov, B., Kim, J.Y., and Ju, J.W. (2025). Nationwide investigation of eukaryotic pathogens in ticks from cattle and sheep in Kyrgyzstan using metabarcoding. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0327953"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1007\/s00436-019-06370-2","article-title":"First molecular survey of piroplasm species in cattle from Kyrgyzstan","volume":"118","author":"Cihan","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kim, Y.J., Seo, J.Y., Park, J.S., Kim, S.Y., Aknazarov, B., Atabekova, N., and Lee, H.I. (2024). Molecular analysis of tick-borne bacterial pathogens from ticks infesting animal hosts in Kyrgyzstan, 2021. Microorganisms, 12.","DOI":"10.3390\/microorganisms12061046"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101817","DOI":"10.1016\/j.ttbdis.2021.101817","article-title":"Tick distribution and detection of Babesia and Theileria species in Eastern and Southern Kazakhstan","volume":"12","author":"Sang","year":"2021","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zeng, W., Kairat, Z., Awulibieer, M., Abylay, S., Serik, K., Yang, M., Wang, Y., and Hazihan, W. (2025). Molecular detection of piroplasms, Anaplasma, and Ehrlichia species in Kazakhstan. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1533589"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11686-023-00658-1","article-title":"The tick-borne pathogens: An overview of China\u2019s situation","volume":"68","author":"Luan","year":"2023","journal-title":"Acta Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Li, Y., Li, J., Li, J., Yang, Y., Kalim, F., Zafar, I., Chahan, B., and Guo, Q. (2026). A molecular epidemiological survey of tick-borne pathogens in dogs and their associated ticks in Xinjiang, China. Animals, 16.","DOI":"10.3390\/ani16040534"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1111\/jvim.12168","article-title":"Review of equine piroplasmosis","volume":"27","author":"Wise","year":"2013","journal-title":"J. Vet. Intern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1017\/S0031182011000060","article-title":"First detection of Babesia occultans in Hyalomma ticks from Tunisia","volume":"138","author":"Bouattour","year":"2011","journal-title":"Parasitology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1051\/parasite\/2014002","article-title":"A review of Hyalomma scupense (Acari, Ixodidae) in the Maghreb region: From biology to control","volume":"21","author":"Gharbi","year":"2014","journal-title":"Parasite"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Antunes, S., Couto, J., Ferrolho, J., Sanches, G.S., Merino Charrez, J.O., De la Cruz Hern\u00e1ndez, N., Mazuz, M., Villar, M., Shkap, V., and de la Fuente, J. (2019). Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.00318"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ozubek, S., Ulucesme, M.C., Cirak, V.Y., and Aktas, M. (2022). Detection of Theileria orientalis genotypes from cattle in Kyrgyzstan. Pathogens, 11.","DOI":"10.3390\/pathogens11101185"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e70522","DOI":"10.1002\/vms3.70522","article-title":"Molecular prevalence and phylogenetic analysis of Theileria ovis and Theileria lestoquardi in small ruminants in Siirt Province, T\u00fcrkiye","volume":"11","author":"Celik","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Altay, K., Erol, U., Sahin, O.F., Aydin, M.F., Aytmirzakizi, A., and Dumanli, N. (2023). First molecular evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in dogs from Kyrgyzstan. Pathogens, 12.","DOI":"10.3390\/pathogens12081046"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/9\/940\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:48:09Z","timestamp":1788947289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/9\/940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":32,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["pathogens15090940"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15090940","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}