{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:37:00Z","timestamp":1792201020686,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T00:00:00Z","timestamp":1460678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Following nosocomial cases of Legionella pneumophila, the investigation of a hot water system revealed that 81.5% of sampled taps were positive for L. pneumophila, despite the presence of protective levels of copper in the water. A significant reduction of L. pneumophila counts was observed by culture after heat shock disinfection. The following corrective measures were implemented to control L. pneumophila: increasing the hot water temperature (55 to 60 \u00b0C), flushing taps weekly with hot water, removing excess lengths of piping and maintaining a water temperature of 55 \u00b0C throughout the system. A gradual reduction in L. pneumophila counts was observed using the culture method and qPCR in the 18 months after implementation of the corrective measures. However, low level contamination was retained in areas with hydraulic deficiencies, highlighting the importance of maintaining a good thermal regime at all points within the system to control the population of L. pneumophila.<\/jats:p>","DOI":"10.3390\/pathogens5020035","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T11:36:02Z","timestamp":1460720162000},"page":"35","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Combination of Heat Shock and Enhanced Thermal Regime to Control the Growth of a Persistent Legionella pneumophila Strain"],"prefix":"10.3390","volume":"5","author":[{"given":"Emilie","family":"B\u00e9dard","sequence":"first","affiliation":[{"name":"Department of Civil Engineering, Polytechnique Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e8s","family":"Boppe","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering, Polytechnique Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Kouam\u00e9","sequence":"additional","affiliation":[{"name":"CIUSSSE\u2014Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1G 2E8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Universit\u00e9 de Sherbrooke, QC J1K 2R1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Pinsonneault","sequence":"additional","affiliation":[{"name":"CIUSSSE\u2014Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1G 2E8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Valiquette","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Universit\u00e9 de Sherbrooke, QC J1K 2R1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jules","family":"Racine","sequence":"additional","affiliation":[{"name":"CIUSSSE\u2014Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1G 2E8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Pr\u00e9vost","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering, Polytechnique Montr\u00e9al, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1093\/cid\/civ870","article-title":"Hospital-wide eradication of a nosocomial Legionella pneumophila serogroup 1 outbreak","volume":"62","author":"Bartley","year":"2016","journal-title":"Clin. 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