{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:14:14Z","timestamp":1789866854461,"version":"4.0.1"},"reference-count":93,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA-CSREES","award":["2008-34476-19161"],"award-info":[{"award-number":["2008-34476-19161"]}]},{"name":"USDA-CSREES","award":["2009-34476-20073"],"award-info":[{"award-number":["2009-34476-20073"]}]},{"name":"USDA HATCH","award":["#OKL02885"],"award-info":[{"award-number":["#OKL02885"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The ability of Listeria monocytogenes to adhere and form biofilms leads to persistence in food processing plants and food-associated listeriosis. The role of specific surface proteins as adhesins to attach Listeria cells to various contact surfaces has not been well characterized to date. In prior research comparing different methods for surface protein extraction, the Ghost urea method revealed cleaner protein content as verified by the least cytoplasmic protein detected in surface extracts using LC-MS\/MS. The same technique was utilized to extract and detect surface proteins among two surface-adherent phenotypic strains of L. monocytogenes (i.e., strongly and weakly adherent). Of 640 total proteins detected among planktonic and sessile cells, 21 protein members were exclusively detected in the sessile cells. Relative LC-MS\/MS detection and quantification of surface-extracted proteins from the planktonic weakly adherent (CW35) and strongly adherent strains (99-38) were examined by protein mass normalization of proteins. We found that L. monocytogenes 99-38 exhibited a total of 22 surface proteins that were over-expressed: 11 proteins were detected in surface extracts of both sessile and planktonic 99-38 that were \u22655-fold over-expressed while another 11 proteins were detected only in planktonic 99-38 cells that were \u226510-fold over-expressed. Our results suggest that these protein members are worthy of further investigation for their involvement as surface adhesins.<\/jats:p>","DOI":"10.3390\/pathogens5020040","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T10:20:11Z","timestamp":1463480411000},"page":"40","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparison of Surface Proteomes of Adherence Variants of Listeria Monocytogenes Using LC-MS\/MS for Identification of Potential Surface Adhesins"],"prefix":"10.3390","volume":"5","author":[{"given":"Hung","family":"Tiong","sequence":"first","affiliation":[{"name":"Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA"},{"name":"Robert M. Kerr Food &amp; Agricultural Products Centre, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Hartson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6448-1769","authenticated-orcid":false,"given":"Peter","family":"Muriana","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA"},{"name":"Robert M. Kerr Food &amp; Agricultural Products Centre, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1042\/bst0250183","article-title":"Bacterial cell surface carbohydrates: Structure and assembly","volume":"25","author":"Hancock","year":"1997","journal-title":"Biochem. Soc. Trans."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/pmic.200400936","article-title":"Analysis of the Listeria cell wall proteome by two-dimensional nanoliquid chromatography coupled to mass spectrometry","volume":"5","author":"Calvo","year":"2005","journal-title":"Proteomics"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1128\/MMBR.00039-06","article-title":"Listeria monocytogenes surface proteins: From genome predictions to function","volume":"71","author":"Bierne","year":"2007","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/MMBR.63.1.174-229.1999","article-title":"Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope","volume":"63","author":"Navarre","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1046\/j.1365-2958.2001.02411.x","article-title":"Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus","volume":"40","author":"Mazmanian","year":"2001","journal-title":"Mol. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S0966-842X(02)02342-9","article-title":"Surface proteins and the pathogenic potential of Listeria monocytogenes","volume":"10","author":"Cabanes","year":"2002","journal-title":"Trends Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1002\/pmic.200900564","article-title":"Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false-positive control","volume":"10","author":"Solis","year":"2010","journal-title":"Proteomics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1084\/jem.167.4.1459","article-title":"Role of hemolysin for the intracellular growth of listeria monocytogenes","volume":"167","author":"Portnoy","year":"1988","journal-title":"J. Exp. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4075","DOI":"10.1128\/iai.65.10.4075-4081.1997","article-title":"The mechanism of cell death in Listeria monocytogenes-infected murine macrophages is distinct from apoptosis","volume":"65","author":"Barsig","year":"1997","journal-title":"Infect. Immun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/j.1462-5822.2005.00665.x","article-title":"Membrane perforations inhibit lysosome fusion by altering ph and calcium in Listeria monocytogenes vacuoles","volume":"8","author":"Shaughnessy","year":"2006","journal-title":"Cell. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1046\/j.1365-2958.1997.4101774.x","article-title":"Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface","volume":"24","author":"Heilmann","year":"1997","journal-title":"Mol. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1146\/annurev-bioeng-061008-124934","article-title":"Proteomics by mass spectrometry: Approaches, advances, and applications","volume":"11","author":"Yates","year":"2009","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1128\/jb.118.2.735-746.1974","article-title":"Autolytic formation of protoplasts (autoplasts) of Streptococcus faecalis 9790: Release of cell wall, autolysin, and formation of stable autoplasts","volume":"118","author":"Joseph","year":"1974","journal-title":"J. Bacteriol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1128\/jb.138.3.699-704.1979","article-title":"Release of autolytic enzyme from Streptococcus faecium cell walls by treatment with dilute alkali","volume":"138","author":"Cornett","year":"1979","journal-title":"J. Bacteriol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6688","DOI":"10.1128\/IAI.67.12.6688-6690.1999","article-title":"A lithium chloride-extracted, broad-spectrum-adhesive 42-kilodalton protein of Staphylococcus epidermidis is ornithine carbamoyltransferase","volume":"67","author":"Hussain","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.mimet.2015.01.004","article-title":"Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by orbitrap mass spectrometry","volume":"110","author":"Tiong","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1002\/pmic.201000808","article-title":"Current methodologies for proteomics of bacterial surface-exposed and cell envelope proteins","volume":"11","author":"Solis","year":"2011","journal-title":"Proteomics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1038\/nature01511","article-title":"Mass spectrometry-based proteomics","volume":"422","author":"Aebersold","year":"2003","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1128\/IAI.72.2.1072-1083.2004","article-title":"New aspects regarding evolution and virulence of Listeria monocytogenes revealed by comparative genomics and DNA arrays","volume":"72","author":"Doumith","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.mib.2003.09.005","article-title":"Genomic analysis of secretion systems","volume":"6","author":"Pallen","year":"2003","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/713609215","article-title":"Analysis of structure and function of putative surface-exposed proteins encoded in the Streptococcus pneumoniae genome: A bioinformatics-based approach to vaccine and drug design","volume":"38","author":"Rigden","year":"2003","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1099\/mic.0.25996-0","article-title":"Characterization of novel lpxtg-containing proteins of Staphylococcus aureus identified from genome sequences","volume":"149","author":"Roche","year":"2003","journal-title":"Microbiology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1007\/s00253-012-4611-1","article-title":"Current knowledge and perspectives on biofilm formation: The case of Listeria monocytogenes","volume":"97","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/j.1462-2920.2010.02378.x","article-title":"Molecular biology of surface colonization by Listeria monocytogenes: An additional facet of an opportunistic Gram-positive foodborne pathogen","volume":"13","author":"Renier","year":"2011","journal-title":"Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.4315\/0362-028X-72.10.2125","article-title":"Adherence characteristics of Listeria strains isolated from three ready-to-eat meat processing plants","volume":"72","author":"Kushwaha","year":"2009","journal-title":"J. Food Prot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"D402","DOI":"10.1093\/nar\/gkj071","article-title":"Leger: Knowledge database and visualization tool for comparative genomics of pathogenic and non-pathogenic Listeria species","volume":"34","author":"Dieterich","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s11483-008-9090-7","article-title":"Attachment strength of Listeria monocytogenes and its internalin-negative mutants","volume":"3","author":"Chen","year":"2008","journal-title":"Food Biophys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1007\/s11483-009-9128-5","article-title":"Positive correlation between the expression of inla and inlb genes of Listeria monocytogenes and its attachment strength on glass surface","volume":"4","author":"Chen","year":"2009","journal-title":"Food Biophys."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1128\/AEM.02419-07","article-title":"Listeria monocytogenes biofilm-associated protein (bapl) may contribute to surface attachment of L. monocytogenes but is absent from many field isolates","volume":"74","author":"Jordan","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.gdata.2014.06.010","article-title":"Genome comparison of Listeria monocytogenes serotype 4a strain hcc23 with selected lineage i and lineage ii L. monocytogenes strains and other Listeria strains","volume":"2","author":"Paul","year":"2014","journal-title":"Genom. Data"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1126\/science.1063447","article-title":"Comparative genomics of Listeria species","volume":"294","author":"Glaser","year":"2001","journal-title":"Science"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1099\/13500872-141-2-261","article-title":"Subtilist: A relational database for the Bacillus subtilis genome","volume":"141","author":"Moszer","year":"1995","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/1471-2105-9-173","article-title":"Locatep: Genome-scale subcellular-location predictor for bacterial proteins","volume":"9","author":"Zhou","year":"2008","journal-title":"BMC Bioinform."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1093\/bioinformatics\/btq249","article-title":"Psortb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes","volume":"26","author":"Yu","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_35","first-page":"285","article-title":"Cw-pred: A hmm-based method for the classification of cell wall-anchored proteins of Gram-positive bacteria","volume":"Volume 7297","author":"Maglogiannis","year":"2012","journal-title":"Artificial intelligence: Theories and applications"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5082","DOI":"10.1021\/pr800162c","article-title":"Prediction of lipoprotein signal peptides in Gram-positive bacteria with a hidden markov model","volume":"7","author":"Bagos","year":"2008","journal-title":"J. Proteom. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1093\/bioinformatics\/17.7.646","article-title":"Evaluation of methods for the prediction of membrane spanning regions","volume":"17","author":"Croning","year":"2001","journal-title":"Bioinformatics"},{"key":"ref_38","first-page":"175","article-title":"A hidden markov model for predicting transmembrane helices in protein sequences","volume":"6","author":"Sonnhammer","year":"1998","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden markov model: Application to complete genomes1","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/protein\/10.1.1","article-title":"Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites","volume":"10","author":"Nielsen","year":"1997","journal-title":"Protein Eng."},{"key":"ref_41","first-page":"122","article-title":"Prediction of signal peptides and signal anchors by a hidden markov model","volume":"6","author":"Nielsen","year":"1998","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","article-title":"Improved prediction of signal peptides: Signalp 3.0","volume":"340","author":"Nielsen","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"Signalp 4.0: Discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/bioinformatics\/btq530","article-title":"Combined prediction of tat and sec signal peptides with hidden markov models","volume":"26","author":"Bagos","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1016\/j.bbamem.2007.07.015","article-title":"Sec- and tat-mediated protein secretion across the bacterial cytoplasmic membrane\u2014Distinct translocases and mechanisms","volume":"1778","author":"Natale","year":"2008","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Biomembr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","article-title":"A simple method for displaying the hydropathic character of a protein","volume":"157","author":"Kyte","year":"1982","journal-title":"J. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7206","DOI":"10.1128\/JB.01041-09","article-title":"Comparative genome analysis of Listeria bacteriophages reveals extensive mosaicism, programmed translational frameshifting, and a novel prophage insertion site","volume":"191","author":"Dorscht","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"11140","DOI":"10.1073\/pnas.92.24.11140","article-title":"An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae","volume":"92","author":"Coomaraswamy","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6192","DOI":"10.1128\/JB.182.21.6192-6202.2000","article-title":"Gene expression analysis of the Streptococcus pneumoniae competence regulons by use of DNA microarrays","volume":"182","author":"Peterson","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4161\/viru.2.5.17703","article-title":"The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle","volume":"2","author":"Camejo","year":"2011","journal-title":"Virulence"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijfoodmicro.2016.02.009","article-title":"Genes involved in Listeria monocytogenes biofilm formation at a simulated food processing plant temperature of 15 \u00b0C","volume":"223","author":"Piercey","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6855","DOI":"10.1128\/AEM.01006-13","article-title":"Characterization of a potential Listeria monocytogenes virulence factor associated with attachment to fresh produce","volume":"79","author":"Bae","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1172\/JCI200320442","article-title":"Quorum sensing in Staphylococcus infections","volume":"112","author":"Yarwood","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1128\/IAI.74.2.1323-1338.2006","article-title":"Intracellular gene expression profile of Listeria monocytogenes","volume":"74","author":"Chatterjee","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.1093\/nar\/gkq162","article-title":"Genome-wide computational identification of wg\/gw argonaute-binding proteins in arabidopsis","volume":"38","author":"Karlowski","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1093\/bioinformatics\/btr128","article-title":"Agos\u2014A universal web tool for gw argonaute-binding domain prediction","volume":"27","author":"Zielezinski","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1677\/joe.0.1470383","article-title":"Endocrine peptides \u2018moonlighting\u2019 as immune modulators: Roles for somatostatin and gh-releasing factor","volume":"147","author":"Campbell","year":"1995","journal-title":"J. Endocrinol."},{"key":"ref_58","first-page":"155","article-title":"Bacterial moonlighting proteins and bacterial virulence","volume":"Volume 358","author":"Dobrindt","year":"2013","journal-title":"Between Pathogenicity and Commensalism"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/S0968-0004(98)01335-8","article-title":"Moonlighting proteins","volume":"24","author":"Jeffery","year":"1999","journal-title":"Trends Biochem. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1084\/jem.176.2.415","article-title":"A major surface protein on group a streptococci is a glyceraldehyde-3-phosphate-dehydrogenase with multiple binding activity","volume":"176","author":"Pancholi","year":"1992","journal-title":"J. Exp. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1128\/IAI.70.3.1254-1259.2002","article-title":"Identification of major outer surface proteins of Streptococcus agalactiae","volume":"70","author":"Hughes","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/1471-2164-12-135","article-title":"Identification of surface proteins in Enterococcus faecalis v583","volume":"12","author":"Bohle","year":"2011","journal-title":"BMC Genom."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1574-695X.2008.00401.x","article-title":"Immunoproteomic assay of surface proteins of streptococcus suis serotype 9","volume":"53","author":"Wu","year":"2008","journal-title":"FEMS Immun. Med. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5290","DOI":"10.1128\/AEM.69.9.5290-5296.2003","article-title":"Effect of acidic pH on expression of surface-associated proteins of streptococcus oralis","volume":"69","author":"Wilkins","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1046\/j.1365-2958.2001.02448.x","article-title":"A-enolase of Streptococcus pneumoniae is a plasmin(ogen)-binding protein displayed on the bacterial cell surface","volume":"40","author":"Bergmann","year":"2001","journal-title":"Mol. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1099\/mic.0.036509-0","article-title":"Lap, an alcohol acetaldehyde dehydrogenase enzyme in Listeria, promotes bacterial adhesion to enterocyte-like caco-2 cells only in pathogenic species","volume":"156","author":"Jagadeesan","year":"2010","journal-title":"Microbiology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.micinf.2009.05.006","article-title":"Expression of lap, a seca2-dependent secretory protein, is induced under anaerobic environment","volume":"11","author":"Burkholder","year":"2009","journal-title":"Microbes Infect."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"D277","DOI":"10.1093\/nar\/gku954","article-title":"Moonprot: A database for proteins that are known to moonlight","volume":"43","author":"Mani","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/IAI.68.9.5002-5010.2000","article-title":"Role of ribosomal protein l12 in gonococcal invasion of hec1b cells","volume":"68","author":"Spence","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1128\/AEM.69.1.258-266.2003","article-title":"Attachment of Listeria monocytogenes to radish tissue is dependent upon temperature and flagellar motility","volume":"69","author":"Gorski","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1128\/AEM.02923-06","article-title":"Temperature-dependent expression of Listeria monocytogenes internalin and internalin-like genes suggests functional diversity of these proteins among the listeriae","volume":"73","author":"McGann","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_72","first-page":"2171","article-title":"Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting","volume":"134","author":"Peel","year":"1988","journal-title":"J. Gen. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"382","DOI":"10.4315\/0362-028X-57.5.382","article-title":"Incidence of Listeria monocytogenes in packages of retail franks","volume":"57","author":"Wang","year":"1994","journal-title":"J. Food Prot."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5235","DOI":"10.1128\/AEM.00114-07","article-title":"Microplate fluorescence assay for measurement of the ability of strains of Listeria monocytogenes from meat and meat-processing plants to adhere to abiotic surfaces","volume":"73","author":"Gamble","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.ijfoodmicro.2009.11.024","article-title":"Comparison of invasiveness among surface-adherent variants of Listeria monocytogenes in caco-2 cell culture assays","volume":"138","author":"Kushwaha","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ijfoodmicro.2010.03.004","article-title":"Analysis of tissue invasiveness of adherent strains of Listeria monocytogenes by in vivo mouse assay","volume":"141","author":"Kushwaha","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the major Listeria monocytogenes serovars by multiplex pcr","volume":"42","author":"Doumith","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.mimet.2005.04.001","article-title":"Multiplex pcr for colony direct detection of Gram-positive histamine- and tyramine-producing bacteria","volume":"63","author":"Coton","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"5328","DOI":"10.1128\/AEM.65.12.5328-5333.1999","article-title":"Listeria monocytogenes Scott A: Cell surface charge, hydrophobicity, and electron donor and acceptor characteristics under different environmental growth conditions","volume":"65","author":"Briandet","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1083\/jcb.41.2.378","article-title":"Isolation of plasma membrane fragments from hela cells","volume":"41","author":"Boone","year":"1969","journal-title":"J. Cell Biol."},{"key":"ref_81","first-page":"139","article-title":"Sequential extraction of proteins by chemical reagents","volume":"Volume 424","author":"Posch","year":"2008","journal-title":"2d Page: Sample Preparation and Fractionation"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0003-2697(84)90782-6","article-title":"A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids","volume":"138","author":"Wessel","year":"1984","journal-title":"Anal. Biochem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage t4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1021\/pr400321x","article-title":"The anticancer drug auy922 generates a proteomics fingerprint that is highly conserved among structurally diverse hsp90 inhibitors","volume":"12","author":"Voruganti","year":"2013","journal-title":"J. Proteom. Res."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/nm.4073","article-title":"Inflammatory signaling in human tuberculosis granulomas is spatially organized","volume":"22","author":"Marakalala","year":"2016","journal-title":"Nat. Med."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.devcel.2014.09.004","article-title":"The intraflagellar transport protein ift27 promotes bbsome exit from cilia through the gtpase arl6\/bbs3","volume":"31","author":"Liew","year":"2014","journal-title":"Dev. Cell"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Mosadeghi, R., Reichermeier, K.M., Winkler, M., Schreiber, A., Reitsma, J.M., Zhang, Y., Stengel, F., Cao, J., Kim, M., and Sweredoski, M.J. (2016). Structural and kinetic analysis of the cop9-signalosome activation and the cullin-ring ubiquitin ligase deneddylation cycle. eLife.","DOI":"10.7554\/eLife.12102.031"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1021\/ac0341261","article-title":"A statistical model for identifying proteins by tandem mass spectrometry","volume":"75","author":"Nesvizhskii","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1021\/ac025747h","article-title":"Empirical statistical model to estimate the accuracy of peptide identifications made by MS\/MS and database search","volume":"74","author":"Keller","year":"2002","journal-title":"Anal. Chem."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1021\/pr0600273","article-title":"Detecting differential and correlated protein expression in label-free shotgun proteomics","volume":"5","author":"Zhang","year":"2006","journal-title":"J. Proteom. Res."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Zhu, W., Smith, J.W., and Huang, C.-M. (2009). Mass spectrometry-based label-free quantitative proteomics. BioMed Res. Int.","DOI":"10.1155\/2010\/840518"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"3368","DOI":"10.1128\/AEM.69.6.3368-3376.2003","article-title":"Development of a Listeria monocytogenes EDGe partial proteome reference map and comparison with the protein profiles of food isolates","volume":"69","author":"Ramnath","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_93","first-page":"3040","article-title":"Proteomic identification of Listeria monocytogenes surface-associated proteins","volume":"13","author":"Zhang","year":"2013","journal-title":"Proteomics"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/5\/2\/40\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:24:02Z","timestamp":1760210642000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/5\/2\/40"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,17]]},"references-count":93,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,6]]}},"alternative-id":["pathogens5020040"],"URL":"https:\/\/doi.org\/10.3390\/pathogens5020040","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,17]]}}}