{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T08:06:25Z","timestamp":1782547585847,"version":"3.54.5"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T00:00:00Z","timestamp":1487116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GO-983-3\/1"],"award-info":[{"award-number":["GO-983-3\/1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Culture of Lower Saxony, Germany"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. Establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. However, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. Our previous isotopologue profiling studies on S. suis grown in porcine blood and cerebrospinal fluid (CSF) revealed conserved activities of central carbon metabolism in both body fluids. On the other hand, they suggested differences in the de novo amino acid biosynthesis. This prompted us to further dissect S. suis adaptation to porcine blood and CSF by RNA deep sequencing (RNA-seq). In blood, the majority of differentially expressed genes were associated with transport of alternative carbohydrate sources and the carbohydrate metabolism (pentose phosphate pathway, glycogen metabolism). In CSF, predominantly genes involved in the biosynthesis of branched-chain and aromatic amino acids were differentially expressed. Especially, isoleucine biosynthesis seems to be of major importance for S. suis in CSF because several related biosynthetic genes were more highly expressed. In conclusion, our data revealed niche-specific metabolic gene activity which emphasizes a selective adaptation of S. suis to host environments.<\/jats:p>","DOI":"10.3390\/pathogens6010007","type":"journal-article","created":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T10:09:07Z","timestamp":1487153347000},"page":"7","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Transcriptomic Analysis Reveals Selective Metabolic Adaptation of Streptococcus suis to Porcine Blood and Cerebrospinal Fluid"],"prefix":"10.3390","volume":"6","author":[{"given":"Anna","family":"Koczula","sequence":"first","affiliation":[{"name":"Center for Infection Medicine, Institute for Microbiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Jarek","sequence":"additional","affiliation":[{"name":"Genome Analytics, Helmholtz Centre for Infection Research, Inhoffensta\u00dfe 7, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Visscher","sequence":"additional","affiliation":[{"name":"Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Valentin-Weigand","sequence":"additional","affiliation":[{"name":"Center for Infection Medicine, Institute for Microbiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Goethe","sequence":"additional","affiliation":[{"name":"Center for Infection Medicine, Institute for Microbiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Willenborg","sequence":"additional","affiliation":[{"name":"Center for Infection Medicine, Institute for Microbiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2217\/fmb.10.2","article-title":"Streptococcus suis: A new emerging or an old neglected zoonotic pathogen?","volume":"5","author":"Gottschalk","year":"2010","journal-title":"Future Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.3201\/eid2007.131594","article-title":"Epidemiology, Clinical Manifestations, and Outcomes of Streptococcus suis Infection in Humans","volume":"20","author":"Huong","year":"2014","journal-title":"Emerg. 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