{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:46:19Z","timestamp":1776465979519,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T00:00:00Z","timestamp":1538697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013206","name":"Food Institutional Research Measure","doi-asserted-by":"publisher","award":["11\/F\/008"],"award-info":[{"award-number":["11\/F\/008"]}],"id":[{"id":"10.13039\/100013206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Listeria monocytogenes is a foodborne pathogen that causes listeriosis, a relatively rare, but potentially fatal, disease, with a mortality rate of 20\u201330%. In general, European Regulations require the absence of L. monocytogenes in five samples of 25 g before the food has left the producer, but if the food has been demonstrated not to support the growth of L. monocytogenes, up to 100 cfu g\u22121 are allowed in the food (except for foods for infants or medical purposes) during its shelf-life under reasonably foreseeable storage conditions. It is important for food producers to determine if their food supports the growth of L. monocytogenes. The European Union Reference Laboratory for L. monocytogenes published a Technical Guidance document for conducting shelf-life studies on L. monocytogenes in ready-to-eat foods in June 2014. Primarily based on the EURL guidance document for conducting challenge studies, the ability of cheese (feta and soft goat\u2019s milk cheese), cold-smoked salmon, coleslaw, and pork pate to support the growth of L. monocytogenes was determined using a starting inoculum of approximately 100 cfu g\u22121. The cheese and pork pate were incubated at 8 \u00b0C for 14 days; the smoked salmon was incubated at 6 \u00b0C for 5 days and 8 \u00b0C for 9 days; and the coleslaw was incubated at 8 \u00b0C for 7 days and 12 \u00b0C for 14 days. The results showed that the smoked salmon and pork pate supported growth, while coleslaw and cheese did not. From this study, it is evident that there are factors in food other than pH, water activity, and total bacterial count (TBC) that can inhibit the ability of L. monocytogenes to grow in food.<\/jats:p>","DOI":"10.3390\/pathogens7040080","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T12:16:44Z","timestamp":1538741804000},"page":"80","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Challenge Studies to Determine the Ability of Foods to Support the Growth of Listeria monocytogenes"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2164-6412","authenticated-orcid":false,"given":"Karen","family":"Hunt","sequence":"first","affiliation":[{"name":"Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjorie","family":"Blanc","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avelino","family":"\u00c1lvarez-Ord\u00f3\u00f1ez","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3017-9622","authenticated-orcid":false,"given":"Kieran","family":"Jordan","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,5]]},"reference":[{"key":"ref_1","unstructured":"De Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H., and Whitman, W. (2009). Listeria. Bergey\u2019s Manual of Systematic Bacteriology, Springer. [2nd ed.]. The Firmicutes."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0168-1605(03)00332-5","article-title":"Influence of the sigB gene on the cold stress survival and subsequent recovery of two Listeria monocytogenes serotypes","volume":"91","author":"Moorhead","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.ijfoodmicro.2010.12.028","article-title":"SigB plays a major role in Listeria monocytogenes tolerance to bile stress","volume":"145","author":"Zhang","year":"2011","journal-title":"Int. J. 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