{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T04:44:35Z","timestamp":1780461875302,"version":"3.54.1"},"reference-count":19,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:00:00Z","timestamp":1551139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Legionnaires\u2019 disease is a potentially fatal pneumonia like infection caused by inhalation or aspiration of water particles contaminated with pathogenic Legionella spp. Household showers have been identified as a potential source of sporadic, community-acquired Legionnaires\u2019 disease. This study used qPCR to enumerate Legionella spp. and Legionella pneumophila in water samples collected from domestic showers across metropolitan Adelaide, South Australia. A survey was used to identify risk factors associated with contamination and to examine awareness of Legionella control in the home. The hot water temperature was also measured. A total of 74.6% (50\/68) and 64.2% (43\/68) showers were positive for Legionella spp. and L. pneumophila, respectively. Statistically significant associations were found between Legionella spp. concentration and maximum hot water temperature (p = 0.000), frequency of shower use (p = 0.000) and age of house (p = 0.037). Lower Legionella spp. concentrations were associated with higher hot water temperatures, showers used at least every week and houses less than 5 years old. However, examination of risk factors associated with L. pneumophila found that there were no statistically significant associations (p &gt; 0.05) with L. pneumophila concentrations and temperature, type of hot water system, age of system, age of house or frequency of use. This study demonstrated that domestic showers were frequently colonized by Legionella spp. and L. pneumophila and should be considered a potential source of sporadic Legionnaires\u2019 disease. Increasing hot water temperature and running showers every week to enable water sitting in pipes to be replenished by the municipal water supply were identified as strategies to reduce the risk of Legionella in showers. The lack of public awareness in this study identified the need for public health campaigns to inform vulnerable populations of the steps they can take to reduce the risk of Legionella contamination and exposure.<\/jats:p>","DOI":"10.3390\/pathogens8010027","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T11:00:44Z","timestamp":1551178844000},"page":"27","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Factors Influencing Legionella Contamination of Domestic Household Showers"],"prefix":"10.3390","volume":"8","author":[{"given":"Deanna","family":"Hayes-Phillips","sequence":"first","affiliation":[{"name":"College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide 5001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bentham","sequence":"additional","affiliation":[{"name":"College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide 5001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5677-9576","authenticated-orcid":false,"given":"Kirstin","family":"Ross","sequence":"additional","affiliation":[{"name":"College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide 5001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8940-3508","authenticated-orcid":false,"given":"Harriet","family":"Whiley","sequence":"additional","affiliation":[{"name":"College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide 5001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,26]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2013). Surveillance for waterborne disease outbreaks associated with drinking water and other nonrecreational water-united states, 2009\u20132010. Morb Mortal Wkly Rep., 62, 714\u2013720."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.jhin.2011.03.022","article-title":"Pontiac fever among retirement home nurses associated with airborne legionella","volume":"78","author":"Hautemaniere","year":"2011","journal-title":"J. Hosp. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.yrtph.2012.11.003","article-title":"Assessing the risk of legionnaires\u2019 disease: The inhalation exposure model and the estimated risk in residential bathrooms","volume":"65","author":"Azuma","year":"2013","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s40572-018-0201-4","article-title":"Outbreaks of legionnaires\u2019 disease and pontiac fever 2006\u20132017","volume":"5","author":"Hamilton","year":"2018","journal-title":"Curr Environ Health Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.ijheh.2016.12.001","article-title":"Occurrence of legionella in uk household showers","volume":"220","author":"Collins","year":"2016","journal-title":"Int. J. Hyg. Environ. Health."},{"key":"ref_6","first-page":"218","article-title":"Investigation of the prevalence of legionella species in domestic hot water systems","volume":"113","author":"Bates","year":"2000","journal-title":"N Z Med J."},{"key":"ref_7","unstructured":"Bartram, J., Chartier, Y., Lee, J.V., Pond, K., and Surman-Lee, S. (2007). Legionella and the Prevention of Legionellosis, World Health Organization."},{"key":"ref_8","unstructured":"United Nations (2015). World Population Ageing 2015: Highlights, Department of Economic and Social Affairs."},{"key":"ref_9","unstructured":"Australian Government (2019, February 22). Australia to 2050: Future Challenges, Available online: https:\/\/www.google.ch\/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=2ahUKEwjHoeCLlM7gAhXBhrQKHWxMAxMQFjAAegQIDhAC&url=http%3A%2F%2Farchive.treasury.gov.au%2Figr%2Figr2010%2Foverview%2Fpdf%2Figr_2010_overview.pdf&usg=AOvVaw3MzWfFlpFPcJoLuPvmlecf."},{"key":"ref_10","unstructured":"Australian Government (2018). Home Care Packages Program: Data Report 1st Quartile 2018\u20132019."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7393","DOI":"10.3390\/ijerph110707393","article-title":"Detection of legionella, l. Pneumophila and mycobacterium avium complex (mac) along potable water distribution pipelines","volume":"11","author":"Whiley","year":"2014","journal-title":"Int. J. Environ. Res. Publ. Health."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1289\/ehp.1408692","article-title":"Epidemiology and ecology of opportunistic premise plumbing pathogens: Legionella pneumophila, mycobacterium avium, and pseudomonas aeruginosa","volume":"123","author":"Falkinham","year":"2015","journal-title":"Environ. Health Perspect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.ijheh.2007.02.004","article-title":"Occurrence of legionella in hot water systems of single-family residences in suburbs of two german cities with special reference to solar and district heating","volume":"211","author":"Mathys","year":"2008","journal-title":"Int. J. Hyg. Environ. Health."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8954","DOI":"10.1021\/acs.est.6b00835","article-title":"Understanding, monitoring, and controlling biofilm growth in drinking water distribution systems","volume":"50","author":"Liu","year":"2016","journal-title":"Environ. Sci. Tech."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.jhin.2007.04.020","article-title":"Disconnecting central hot water and using electric showers to avoid colonization of the water system by legionella pneumophila: An 11-year study","volume":"66","author":"Oliveira","year":"2007","journal-title":"J. Hosp. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"33998","DOI":"10.1038\/srep33998","article-title":"Characterization of aerosols containing legionella generated upon nebulization","volume":"6","author":"Allegra","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8944","DOI":"10.1128\/AEM.71.12.8944-8948.2005","article-title":"Legionella confirmation using real-time pcr and syto9 is an alternative to current methodology","volume":"71","author":"Giglio","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1365-2672.2012.05305.x","article-title":"Effectiveness of qpcr permutations, internal controls and dilution as means for minimizing the impact of inhibition while measuring enterococcus in environmental waters","volume":"113","author":"Cao","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_19","first-page":"4215","article-title":"Detection of legionella pneumophilausing a real-time pcr hybridization assay","volume":"38","author":"Ballard","year":"2000","journal-title":"J. Med. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/8\/1\/27\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:34:43Z","timestamp":1760186083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/8\/1\/27"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,26]]},"references-count":19,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,3]]}},"alternative-id":["pathogens8010027"],"URL":"https:\/\/doi.org\/10.3390\/pathogens8010027","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,26]]}}}