{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:22:49Z","timestamp":1777422169944,"version":"3.51.4"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Anaplasma phagocytophilum and Rickettsia spp. are tick-borne bacteria of veterinary and human concern. In view of the One-Health concept, the present study wanted to evaluate the spreading of these pathogens in horses living in central Italy. In particular, the aim of the investigation was to verify the exposure to A. phagocytophilum in order to update the prevalence of this pathogen in the equine population from this area, and to spotted fever group (SFG) Rickettsia spp. to evaluate a possible role of horses in the epidemiology of rickettsiosis. Indirect immunofluorescent assay was carried out to detect antibodies against A. phagocytophilum and SFG (spotted fever group) Rickettsia spp. in blood serum samples collected from 479 grazing horses living in central Italy during the period from 2013 to 2018. One hundred and nine (22.75%) horses were positive for A. phagocytophilum, 72 (15.03%) for SFG Rickettsia spp., and 19 (3.96%) for both antigens. The obtained results confirm the occurrence of A. phagocytophilum in equine populations, and also suggest the involvement of horses in the epidemiology of SFG rickettsiosis. In both cases, in view of the zoonotic aspect of these pathogens and the frequent contact between horses and humans, the monitoring of equine populations could be useful for indication about the spreading of the tick-borne pathogens in a certain geographic area.<\/jats:p>","DOI":"10.3390\/pathogens8030088","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T07:27:17Z","timestamp":1561534037000},"page":"88","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Serological Evidence of Anaplasma phagocytophilum and Spotted Fever Group Rickettsia spp. Exposure in Horses from Central Italy"],"prefix":"10.3390","volume":"8","author":[{"given":"Valentina Virginia","family":"Ebani","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, viale delle Piagge 2, 56124 Pisa, Italy"},{"name":"CIRSEC, Center for Climatic Change Impact, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","article-title":"Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia, and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and \u201cHGE agent\u201d as subjective synonyms of Ehrlichia phagocytophila","volume":"51","author":"Dumler","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2013.00031","article-title":"Anaplasma phagocytophilum\u2014A wide spread multi-host pathogen with highly adaptive strategies","volume":"3","author":"Stuen","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.idc.2015.02.007","article-title":"Human granulocytic anaplasmosis","volume":"29","author":"Bakken","year":"2015","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_4","first-page":"232","article-title":"Acute clinical, hematologic, serologic, and polymerase chain reaction findings in horses experimentally infected with a European strain of Anaplasma phagocytophilum","volume":"9","author":"Aspan","year":"2005","journal-title":"J. Vet. Intern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1574-695X.2006.00138.x","article-title":"Spotted fever rickettsioses in southern and Eastern Europe","volume":"49","author":"Brouqui","year":"2007","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"318","DOI":"10.4269\/ajtmh.2008.78.318","article-title":"Socio-demographic and climatic factors as correlates of Mediterranean spotted fever (MSF) in northern Sardinia","volume":"78","author":"Vescio","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"983","DOI":"10.3201\/eid0809.020600","article-title":"First detection of spotted fever group rickettsiae in Ixodes ricinus from Italy","volume":"8","author":"Beninati","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3201\/eid1201.050850","article-title":"Rickettsia massiliae human isolation","volume":"12","author":"Vitale","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1089\/vbz.2008.0006","article-title":"Detection and identification of Rickettsia species in the northeast of Italy","volume":"8","author":"Floris","year":"2008","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1111\/j.1469-0691.2008.02082.x","article-title":"First direct detection of rickettsial pathogens and a new rickettsia, \u201cCandidatus Rickettsia barbariae\u201d, in ticks from Sardinia, Italy","volume":"14","author":"Mura","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1603\/033.046.0636","article-title":"Rickettsia slovaca and Rickettsia raoultii in Dermacentor marginatus ticks collected on wild boars in Tuscany, Italy","volume":"46","author":"Selmi","year":"2009","journal-title":"J. Med. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ttbdis.2012.10.031","article-title":"Detection of Leishmania infantum, Babesia canis, and rickettsiae in ticks removed from dogs living in Italy","volume":"3","author":"Trotta","year":"2012","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1089\/vbz.2015.1768","article-title":"Serological and molecular prevalence of Rickettsia helvetica and Anaplasma phagocytophilum in wild cervids and domestic mammals in the Central parts of Sweden","volume":"15","author":"Elfving","year":"2015","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s10493-016-0027-4","article-title":"Molecular evidence for bacterial pathogens in Ixodes ricinus ticks infesting Shetland ponies","volume":"69","author":"Skotarczak","year":"2016","journal-title":"Exp. Appl. Acarol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick-borne rickettsioses around the world: A geographic approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6418","DOI":"10.1128\/AEM.71.10.6418-6422.2005","article-title":"Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States","volume":"71","author":"Alberti","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.cimid.2008.08.002","article-title":"Anaplasma phagocytophilum in horses and tick: A preliminary survey of central Italy","volume":"33","author":"Passamonti","year":"2010","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1007\/s00436-012-2854-5","article-title":"Anaplasma phagocytophilum seroprevalence in equids: A survey in Sicily (Italy)","volume":"111","author":"Giudice","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1292\/jvms.12-0449","article-title":"Prevalence of tick borne pathogens in horses from Italy","volume":"75","author":"Laus","year":"2013","journal-title":"J. Vet. Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.jevs.2015.01.017","article-title":"Tick-borne infections in horses from Tuscany, Italy","volume":"35","author":"Ebani","year":"2015","journal-title":"J. Equine Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"899","DOI":"10.20506\/rst.24.3.1612","article-title":"An epidemiological survey of equine anaplasmosis (Anaplasma phagocytophilum) in southern France","volume":"24","author":"Leblond","year":"2005","journal-title":"Rev. Sci. Tech."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1007\/s00436-013-3429-9","article-title":"Prevalence of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses from the north of Portugal","volume":"112","author":"Ribeiro","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1089\/vbz.2018.2293","article-title":"Serological evidence of Borrelia burgdorferi, Anaplasma phagocytophilum and Ehrlichia spp. infections on horses from Southeastern Bulgaria","volume":"18","author":"Tsachev","year":"2018","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.1600-0463.2004.apm11204-0503.x","article-title":"Detection of Anaplasma phagocytophilum in animals by real-time polymerase chain reaction","volume":"112","year":"2004","journal-title":"APMIS"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1136\/vr.162.7.216","article-title":"Anaplasma phagocytophilum infection in horses in the Netherlands","volume":"162","author":"Butler","year":"2008","journal-title":"Vet. Rec."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.vetpar.2015.10.025","article-title":"Molecular surveillance of Theileria equi and Anaplasma phagocytophilum infections in horses from Ukraine, Poland and Slovakia","volume":"215","author":"Slivinska","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/s13071-017-2221-6","article-title":"Prevalence and genetic variability of Anaplasma phagocytophilum in wild rodents from the Italian Alps","volume":"10","author":"Rosso","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_28","first-page":"73","article-title":"Anaplasma phagocytophilum infection in thrombocytopenic dogs","volume":"54","author":"Balboni","year":"2018","journal-title":"Vet. Ital."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cimid.2016.06.003","article-title":"Molecular survey on the presence of zoonotic arthropod-borne pathogens in wild red deer (Cervus elaphus)","volume":"47","author":"Ebani","year":"2016","journal-title":"Comp. Immunol. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.actatropica.2017.05.014","article-title":"Molecular detection of tick-borne pathogens in wild red foxes (Vulpes vulpes) from Central Italy","volume":"172","author":"Ebani","year":"2017","journal-title":"Acta Trop."},{"key":"ref_31","first-page":"413","article-title":"Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy","volume":"48","author":"Aureli","year":"2012","journal-title":"Vet. Ital."},{"key":"ref_32","unstructured":"Sellon, C.D., and Long, M.T. (2007). Anaplasma Phagocytophila. Equine Infectious Disease, Saunders."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ttbdis.2018.09.001","article-title":"Mediterranean spotted fever rickettsiosis in Italy, 2001-2015: Spatio-temporal distribution based on hospitalization records","volume":"10","author":"Vescio","year":"2019","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_34","first-page":"521","article-title":"Molecular survey of Rickettsia spp.","volume":"55","author":"Trotta","year":"2008","journal-title":"in sick dogs in Italy. Zoonoses Public Health"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s13071-015-0824-3","article-title":"Acute febrile illness is associated with Rickettsia spp. infection in dogs","volume":"8","author":"Pennisi","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5004","DOI":"10.21897\/rmvz.14","article-title":"Antibodies against spotted fever group Rickettsia sp. in horses of the Colombian Orinoquia","volume":"20","author":"Acevedo","year":"2015","journal-title":"Rev. MVZ Cordoba"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Souza, C.E., Bonato Camargo, L., Pinter, A., and Donalisio, M.R. (2016). High seroprevalence for Rickettsia rickettsii in equine suggests risk of human infection in silent areas for the Brazilian Spotted Fever. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0153303"},{"key":"ref_38","first-page":"1","article-title":"Exposure of Baixadeiro horses ro Rickettsia spp. and to ticks infected by Rickettsia amblyommatis in the Baixada Maranhense micro-region, Maranh\u00e3o, Brazil","volume":"48","author":"Filho","year":"2018","journal-title":"Cienc. Rural"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1186\/s13071-016-1784-y","article-title":"Experimental infection of horses with Rickettsia rickettsia","volume":"9","author":"Ueno","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijid.2014.11.001","article-title":"Incidence of Mediterranean spotted fever in Sicilian children: A clinical-epidemiological observational retrospective study from 1987 to 2010","volume":"31","author":"Vitaliti","year":"2015","journal-title":"Int. J. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1186\/s13071-016-1639-6","article-title":"Exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast Italy","volume":"9","author":"Vascellari","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1128\/jcm.35.11.2715-2727.1997","article-title":"Laboratory diagnosis of rickettsioses: Current approaches to the diagnosis of old and new rickettsial diseases","volume":"35","author":"Raoult","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s00508-012-0217-y","article-title":"Contact with horses is a risk factor for tick-borne lymphadenopathy (TIBOLA): A case control study","volume":"124","author":"Lakos","year":"2012","journal-title":"Wien. Klin. Wochenschr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1136\/vr.160.4.122","article-title":"Death of a horse infected experimentally with Anaplasma phagocytophilum","volume":"160","author":"Franzen","year":"2007","journal-title":"Vet. Rec."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/8\/3\/88\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:01:24Z","timestamp":1760187684000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/8\/3\/88"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,26]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["pathogens8030088"],"URL":"https:\/\/doi.org\/10.3390\/pathogens8030088","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,26]]}}}