{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T20:52:16Z","timestamp":1778619136469,"version":"3.51.4"},"reference-count":60,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Recently discovered extraintestinal Escherichia fergusonii obtained from non-clinical samples has exhibited the potential for acquiring multiple beta-lactamase genes, just like many extraintestinal Escherichia coli strains. Albeit, they are often omitted or classified as E. coli. This study aimed to, therefore, identify carbapenem-resistant extended-spectrum beta-lactamase (ESBL) producing E. fergusonii isolates from clinical samples, determine their evolutionary relatedness using 16S rRNA sequencing analysis and screen for beta-lactamase genes. A total of 135 septic wound samples were obtained from patients on referral at a General Hospital in Lagos, Nigeria. For the phenotypic identification of isolates from culture-positive samples, morphological, and physiological tests were carried out. Identities of the isolates harbouring beta-lactamase genes were assigned to their genus strains using the 16S rRNA sequencing. The Kirby Bauer disc diffusion technique and double-disc synergy test were used to screen isolates for multidrug resistance and ESBL production. Carbapenem-resistant ESBL producing isolates were screened for beta-lactamase genes in a polymerase chain reaction. Three E. fergusonii isolates (CR11, CR35 and CR49) were obtained during this study. E. fergusonii strains were motile, non-lactose and non-sorbitol fermenting but positive for cellobiose and adonitol fermentation. The I6S rRNA assigned the phenotypically identified isolates to E. fergusonii species. All three isolates were multidrug-resistant, carbapenem-resistant and ESBL producers. Isolates CR11 and CR35 harboured cefotaximase (CTX-M) and temoniera (TEM) beta-lactamase genes while CR49 harboured sulfhydryl variable (SHV) beta-lactamase gene. We herein report the detection of multiple beta-lactamase genes in carbapenem-resistant ESBL producing E. fergusonii from clinical samples.<\/jats:p>","DOI":"10.3390\/pathogens8040164","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T03:06:51Z","timestamp":1569467211000},"page":"164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["First Detection of Carbapenem-Resistant Escherichia fergusonii Strains Harbouring Beta-Lactamase Genes from Clinical Samples"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6679-5377","authenticated-orcid":false,"given":"Tomilola","family":"Adesina","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9314-6460","authenticated-orcid":false,"given":"Obinna","family":"Nwinyi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nandita","family":"De","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olayemi","family":"Akinnola","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Omonigbehin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.UTI-0006-2012","article-title":"Reservoirs of Extraintestinal Pathogenic Escherichia coli","volume":"3","author":"Manges","year":"2015","journal-title":"Microbiol. 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