{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:18:39Z","timestamp":1792192719201,"version":"4.4.0"},"reference-count":20,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T00:00:00Z","timestamp":1578182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Streptococcus suis naturally inhabits the tonsils and nasal cavities of pigs. Some strains can cause systemic infection, leading to a wide range of diseases. A case-control study was conducted to (i) examine serotypes isolated from systemic sites (blood\/meninges\/spleen) in cases, (ii) determine whether serotypes in systemic sites were found in upper respiratory sites (tonsil\/nasal cavity) of the same cases, and (iii) determine the serotypes in upper respiratory sites of case and farm and pen- matched controls. In total, 606 samples from 128 pigs were cultured for S. suis. The isolates were examined for presence of gdh and recN genes by polymerase chain reaction (PCR) and were identified as S. suis if both genes were present. The S. suis isolates were then serotyped using a two step-multiplex PCR. Serotypes 9 (n = 9), (2,1\/2) (n = 7) and untypable isolates (n = 7) were most commonly found in systemic sites. Detection of serotypes 9 (p = 0.03) in upper respiratory sites were positively associated with their detection in systemic sites of cases, while a trend was seen with serotype (2,1\/2) (p = 0.07). Last, no association between serotypes recovered from upper respiratory sites of cases and controls could be detected. Untypable isolates were detected in high frequency, which warrants further investigation. This study confirms that a variety of serotypes can be found in commercial swine production and shows a difference in serotypes recovered from systemic sites in pigs with clinical signs of S. suis infections.<\/jats:p>","DOI":"10.3390\/pathogens9010044","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T03:48:48Z","timestamp":1578282528000},"page":"44","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Case-Control Study to Investigate the Serotypes of S. suis Isolates by Multiplex PCR in Nursery Pigs in Ontario, Canada"],"prefix":"10.3390","volume":"9","author":[{"given":"Leann C.","family":"Denich","sequence":"first","affiliation":[{"name":"Department of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9612-0263","authenticated-orcid":false,"given":"Abdolvahab","family":"Farzan","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Friendship","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Arndt","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Gottschalk","sequence":"additional","affiliation":[{"name":"The Research Group on Infectious Diseases in Production Animals, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, Montr\u00e9al, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zvonimir","family":"Poljak","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0378-1135(00)00250-9","article-title":"The pathogenesis of the meningitis caused by Streptococcus suis: The unresolved questions","volume":"76","author":"Gottschalk","year":"2000","journal-title":"Vet. 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