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Because of this, leishmaniasis, a disease caused by these flagellated protists, is ranked as one of the most serious tropical infections worldwide. Neither prophylactic medication, nor vaccination has been developed thus far, even though the infection has usually led to strong and long-lasting immunity. In this paper, we describe a \u201csuicidal\u201d system established in Leishmania mexicana, a human pathogen causing cutaneous leishmaniasis. This system is based on the expression and (de)stabilization of a basic phospholipase A2 toxin from the Bothrops pauloensis snake venom, which leads to the inducible cell death of the parasites in vitro. Furthermore, the suicidal strain was highly attenuated during macrophage infection, regardless of the toxin stabilization. Such a deliberately weakened parasite could be used to vaccinate the host, as its viability is regulated by the toxin stabilization, causing a profoundly reduced pathogenesis.<\/jats:p>","DOI":"10.3390\/pathogens9020079","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T07:41:11Z","timestamp":1580110871000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Suicidal Leishmania"],"prefix":"10.3390","volume":"9","author":[{"given":"Lucie","family":"Pode\u0161vov\u00e1","sequence":"first","affiliation":[{"name":"Life Science Research Centre and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tereza","family":"Le\u0161tinov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 44 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3730-8979","authenticated-orcid":false,"given":"Eva","family":"Hor\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice (Budweis), Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0578-6618","authenticated-orcid":false,"given":"Julius","family":"Luke\u0161","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice (Budweis), Czech Republic"},{"name":"Faculty of Sciences, University of South Bohemia, 370 05 \u010cesk\u00e9 Bud\u011bjovice (Budweis), Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1790-1123","authenticated-orcid":false,"given":"Petr","family":"Volf","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 44 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4765-3263","authenticated-orcid":false,"given":"Vyacheslav","family":"Yurchenko","sequence":"additional","affiliation":[{"name":"Life Science Research Centre and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic"},{"name":"Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov University, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bruschi, F., and Gradoni, L. (2018). The Leishmaniases: Old Neglected Tropical Diseases, Springer.","DOI":"10.1007\/978-3-319-72386-0"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182018000951","article-title":"Recent advances in trypanosomatid research: Genome organization, expression, metabolism, taxonomy and evolution","volume":"146","author":"Maslov","year":"2019","journal-title":"Parasitology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1186\/1756-3305-5-276","article-title":"Leishmania development in sand flies: Parasite-vector interactions overview","volume":"5","author":"Volf","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.2217\/fmb.14.103","article-title":"Leishmania and the macrophage: A multifaceted interaction","volume":"10","author":"Podinovskaia","year":"2015","journal-title":"Future Microbiol."},{"key":"ref_5","unstructured":"WHO (2020, January 24). Available online: http:\/\/www.who.int\/gho\/neglected_diseases\/leishmaniasis\/en\/."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.actatropica.2011.02.007","article-title":"Systematic review of the adverse effects of cutaneous leishmaniasis treatment in the New World","volume":"118","author":"Oliveira","year":"2011","journal-title":"Acta Trop."},{"key":"ref_7","first-page":"1","article-title":"An overview on Leishmania vaccines: A narrative review article","volume":"Volume 6","author":"Rezvan","year":"2015","journal-title":"Veterinary Research Forum"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1016\/j.vaccine.2012.11.065","article-title":"Leishmanization revisited: Immunization with a naturally attenuated cutaneous Leishmania donovani isolate from Sri Lanka protects against visceral leishmaniasis","volume":"31","author":"McCall","year":"2013","journal-title":"Vaccine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1016\/j.vaccine.2005.02.015","article-title":"Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis","volume":"23","author":"Khamesipour","year":"2005","journal-title":"Vaccine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1128\/CMR.14.2.229-243.2001","article-title":"Leishmaniasis: Current status of vaccine development","volume":"14","author":"Handman","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4103\/0974-777X.62881","article-title":"Leishmaniasis vaccine: Where are we today?","volume":"2","author":"Kedzierski","year":"2010","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11627","DOI":"10.1038\/s41598-018-30076-7","article-title":"Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice","volume":"8","author":"Santi","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.4049\/jimmunol.1202801","article-title":"Live attenuated Leishmania donovani p27 gene knockout parasites are nonpathogenic and elicit long-term protective immunity in BALB\/c mice","volume":"190","author":"Dey","year":"2013","journal-title":"J. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.4049\/jimmunol.0900276","article-title":"Intracellular replication-deficient Leishmania donovani induces long lasting protective immunity against visceral leishmaniasis","volume":"183","author":"Selvapandiyan","year":"2009","journal-title":"J. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10706","DOI":"10.1038\/srep10706","article-title":"Genetically engineered ascorbic acid-deficient live mutants of Leishmania donovani induce long lasting protective immunity against visceral leishmaniasis","volume":"5","author":"Anand","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Solana, J.C., Ramirez, L., Corvo, L., de Oliveira, C.I., Barral-Netto, M., Requena, J.M., Iborra, S., and Soto, M. (2017). Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005644"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/expr.1996.4115","article-title":"Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene","volume":"85","author":"Muyombwe","year":"1997","journal-title":"Exp. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"22997","DOI":"10.1074\/jbc.273.36.22997","article-title":"Inducible expression of suicide genes in Leishmania donovani amastigotes","volume":"273","author":"Ghedin","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.vaccine.2004.08.032","article-title":"Development of a recombinant Leishmania major strain sensitive to ganciclovir and 5-fluorocytosine for use as a live vaccine challenge in clinical trials","volume":"23","author":"Davoudi","year":"2005","journal-title":"Vaccine"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/eji.200838389","article-title":"Photodynamic vaccination of hamsters with inducible suicidal mutants of Leishmania amazonensis elicits immunity against visceral leishmaniasis","volume":"39","author":"Kumari","year":"2009","journal-title":"Eur. J. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.micinf.2015.04.003","article-title":"Inducible suicide vector systems for Trypanosoma cruzi","volume":"17","author":"Ma","year":"2015","journal-title":"Microbes Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.molbiopara.2017.03.008","article-title":"Inducible protein stabilization system in Leishmania mexicana","volume":"214","author":"Podesvova","year":"2017","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1017\/S0031182013000012","article-title":"BnSP-7 toxin, a basic phospholipase A2 from Bothrops pauloensis snake venom, interferes with proliferation, ultrastructure and infectivity of Leishmania (Leishmania) amazonensis","volume":"140","author":"Nunes","year":"2013","journal-title":"Parasitology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0022-1759(95)00072-I","article-title":"A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V","volume":"184","author":"Vermes","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"ref_25","first-page":"73970J","article-title":"Toxins and antimicrobial peptides: Interactions with membranes","volume":"7397","author":"Schlamadinger","year":"2009","journal-title":"Proc. SPIE Int. Soc. Opt. Eng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e00715-19","DOI":"10.1128\/mBio.00715-19","article-title":"Antimicrobial peptides, polymorphic toxins, and self-nonself recognition systems in Archaea: An untapped armory for intermicrobial conflicts","volume":"10","author":"Makarova","year":"2019","journal-title":"MBio"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Luk\u0161a, J., Ravoityt\u0117, B., Konovalovas, A., Aitmanait\u0117, L., Butenko, A., Yurchenko, V., Serva, S., and Servien\u0117, E. (2017). Different metabolic pathways are involved in response of Saccharomyces cerevisiae to L-A and M viruses. Toxins, 9.","DOI":"10.3390\/toxins9080233"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.molcel.2018.01.003","article-title":"Toxins, targets, and triggers: An overview of toxin-antitoxin biology","volume":"70","author":"Harms","year":"2018","journal-title":"Mol. Cell"},{"key":"ref_29","first-page":"549","article-title":"Drug discovery in infectious diseases","volume":"Volume 7","author":"Selzer","year":"2016","journal-title":"Comprehensive Analysis of Parasite Biology: From Metabolism to Drug Discovery"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ibmb.2004.10.007","article-title":"An antimicrobial peptide with trypanocidal activity characterized from Glossina morsitans morsitans","volume":"35","author":"Hu","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.exppara.2010.02.016","article-title":"Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts","volume":"125","author":"Fieck","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.peptides.2011.01.011","article-title":"Leishmanicidal activity of synthetic antimicrobial peptides in an infection model with human dendritic cells","volume":"32","author":"Lozano","year":"2011","journal-title":"Peptides"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lynn, M.A., Kindrachuk, J., Marr, A.K., Jenssen, H., Pante, N., Elliott, M.R., Napper, S., Hancock, R.E., and McMaster, W.R. (2011). Effect of BMAP-28 antimicrobial peptides on Leishmania major promastigote and amastigote growth: Role of leishmanolysin in parasite survival. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001141"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0005-2736(99)00198-4","article-title":"Diversity of antimicrobial peptides and their mechanisms of action","volume":"1462","author":"Epand","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/CMR.00056-05","article-title":"Peptide antimicrobial agents","volume":"19","author":"Jenssen","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1083\/jcb.200105081","article-title":"alpha-Toxin is a mediator of Staphylococcus aureus-induced cell death and activates caspases via the intrinsic death pathway independently of death receptor signaling","volume":"155","author":"Bantel","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1126\/science.274.5294.1859","article-title":"Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore","volume":"274","author":"Song","year":"1996","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"860","DOI":"10.2174\/092986609788923356","article-title":"The phospholipase A2 homologues of snake venoms: Biological activities and their possible adaptive roles","volume":"16","author":"Lomonte","year":"2009","journal-title":"Protein Pept. Lett."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/0041-0101(95)00085-Z","article-title":"Phospholipase A2 myotoxins from Bothrops snake venoms","volume":"33","author":"Lomonte","year":"1995","journal-title":"Toxicon"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1016\/S0041-0101(01)00141-6","article-title":"Identification of the myotoxic site of the Lys49 phospholipase A(2) from Agkistrodon piscivorus piscivorus snake venom: Synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities","volume":"39","author":"Angulo","year":"2001","journal-title":"Toxicon"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.ijbiomac.2017.04.013","article-title":"BmajPLA2-II, a basic Lys49-phospholipase A2 homologue from Bothrops marajoensis snake venom with parasiticidal potential","volume":"102","author":"Grabner","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00436-007-0871-6","article-title":"The effect of phospholipase A2 from Crotalus durissus collilineatus on Leishmania (Leishmania) amazonensis infection","volume":"102","author":"Passero","year":"2008","journal-title":"Parasitol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.2174\/1568026619666190723154756","article-title":"Isolation, biochemical characterization and antiparasitic activity of BmatTX-IV, a basic Lys49-phospholipase A2 from the venom of Bothrops mattogrossensis from Paraguay","volume":"19","author":"Alfonso","year":"2019","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"404","DOI":"10.15698\/mic2018.09.646","article-title":"Temporal analysis of the autophagic and apoptotic phenotypes in Leishmania parasites","volume":"5","author":"Basmaciyan","year":"2018","journal-title":"Microb. Cell"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Weing\u00e4rtner, A., Kemmer, G., Muller, F.D., Zampieri, R.A., dos Santos, M.G., Schiller, J., and Pomorski, T.G. (2012). Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0042070"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"E4639","DOI":"10.1073\/pnas.1608644113","article-title":"Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination","volume":"113","author":"Braten","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1006\/expr.1993.1050","article-title":"Leishmania mexicana: Induction of metacyclogenesis by cultivation of promastigotes at acidic pH","volume":"76","author":"Bates","year":"1993","journal-title":"Exp. Parasitol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0169-4758(93)90181-E","article-title":"Axenic culture of Leishmania amastigotes","volume":"9","author":"Bates","year":"1993","journal-title":"Parasitol. Today"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Ishemgulova, A., Kraeva, N., Hlavacova, J., Zimmer, S.L., Butenko, A., Podesvova, L., Lestinova, T., Lukes, J., Kostygov, A., and Votypka, J. (2017). A putative ATP\/GTP binding protein affects Leishmania mexicana growth in insect vectors and vertebrate hosts. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005782"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.molbiopara.2014.11.002","article-title":"Tetracycline-inducible gene expression system in Leishmania mexicana","volume":"198","author":"Kraeva","year":"2014","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kraeva, N., Butenko, A., Hlav\u00e1\u010dov\u00e1, J., Kostygov, A., My\u0161kova, J., Grybchuk, D., Le\u0161tinov\u00e1, T., Vot\u00fdpka, J., Volf, P., and Opperdoes, F. (2015). Leptomonas seymouri: Adaptations to the dixenous life cycle analyzed by genome sequencing, transcriptome profiling and co-infection with Leishmania donovani. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1005127"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Kraeva, N., Le\u0161tinov\u00e1, T., Ishemgulova, A., Majerov\u00e1, K., Butenko, A., Vaselek, S., Bespyatykh, J., Charyyeva, A., Spitzov\u00e1, T., and Kostygov, A.Y. (2019). LmxM.22.0250-encoded dual specificity protein\/lipid phosphatase impairs Leishmania mexicana virulence in vitro. Pathogens, 8.","DOI":"10.3390\/pathogens8040241"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/2\/79\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:44:38Z","timestamp":1760363078000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/2\/79"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,25]]},"references-count":52,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["pathogens9020079"],"URL":"https:\/\/doi.org\/10.3390\/pathogens9020079","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,25]]}}}