{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:44:44Z","timestamp":1788273884416,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T00:00:00Z","timestamp":1582156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI15\/1581"],"award-info":[{"award-number":["PI15\/1581"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["PI15\/1581"],"award-info":[{"award-number":["PI15\/1581"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CB06\/02\/0053"],"award-info":[{"award-number":["CB06\/02\/0053"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Secretar\u00eda de Pol\u00edticas Universitarias, Ministerio de Educaci\u00f3n y Deportes, Rep\u00fablica Argentina","award":["PPID\/M007-UNLP 2016"],"award-info":[{"award-number":["PPID\/M007-UNLP 2016"]}]},{"name":"Centros de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBER-ESP)","award":["CB06\/02\/0053"],"award-info":[{"award-number":["CB06\/02\/0053"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The study\u2019s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010\u20132014). Species identification (biochemical testing\/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE\/MLST\/BAPS) were determined according to standard guidelines. \u03b2-lactamase production was determined by a nitrocefin test and confirmed by PCR\/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9\/ST179\/ST236\/ST281\/ST388\/ST604\/ST720), were resistant to high-levels (HLR) of gentamicin\/streptomycin. A ST9 clone (bla+\/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25\/ST18\/ST19\/ST52\/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792\/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.<\/jats:p>","DOI":"10.3390\/pathogens9020142","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T08:59:47Z","timestamp":1582275587000},"page":"142","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Detection of \u03b2-Lactamase-Producing Enterococcus faecalis and Vancomycin-Resistant Enterococcus faecium Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina"],"prefix":"10.3390","volume":"9","author":[{"given":"Celia M.","family":"Schell","sequence":"first","affiliation":[{"name":"Centro Universitario de Estudios Microbiol\u00f3gicos y Parasitol\u00f3gicos (CUDEMyP), Centro Universidad Nacional de La Plata asociado a Comisi\u00f3n de Investigaciones Cient\u00edficas (CIC), Facultad de Ciencias M\u00e9dicas, Universidad Nacional de La Plata. Av. 60 y 120 s\/n, 3er piso, CP 1900 La Plata, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1016-7173","authenticated-orcid":false,"given":"Ana P.","family":"Tedim","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), Carretera de Colmenar, km. 9.1, Planta -1IZQ, 28034 Madrid, Spain"},{"name":"Centros de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBER-ESP), Av. Monforte de Lemos, 3-5. Pabell\u00f3n 11. Planta 0, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Rodr\u00edguez-Ba\u00f1os","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), Carretera de Colmenar, km. 9.1, Planta -1IZQ, 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica D.","family":"Sparo","sequence":"additional","affiliation":[{"name":"Centro Universitario de Estudios Microbiol\u00f3gicos y Parasitol\u00f3gicos (CUDEMyP), Centro Universidad Nacional de La Plata asociado a Comisi\u00f3n de Investigaciones Cient\u00edficas (CIC), Facultad de Ciencias M\u00e9dicas, Universidad Nacional de La Plata. Av. 60 y 120 s\/n, 3er piso, CP 1900 La Plata, Buenos Aires, Argentina"},{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Hospital Municipal Ram\u00f3n Santamarina, Gral. Paz 1406, B7000 Tandil, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Lissarrague","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Hospital Municipal Ram\u00f3n Santamarina, Gral. Paz 1406, B7000 Tandil, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Basualdo","sequence":"additional","affiliation":[{"name":"Centro Universitario de Estudios Microbiol\u00f3gicos y Parasitol\u00f3gicos (CUDEMyP), Centro Universidad Nacional de La Plata asociado a Comisi\u00f3n de Investigaciones Cient\u00edficas (CIC), Facultad de Ciencias M\u00e9dicas, Universidad Nacional de La Plata. Av. 60 y 120 s\/n, 3er piso, CP 1900 La Plata, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa M.","family":"Coque","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), Carretera de Colmenar, km. 9.1, Planta -1IZQ, 28034 Madrid, Spain"},{"name":"Centros de Investigaci\u00f3n Biom\u00e9dica en Red de Epidemiolog\u00eda y Salud P\u00fablica (CIBER-ESP), Av. Monforte de Lemos, 3-5. Pabell\u00f3n 11. Planta 0, 28029 Madrid, Spain"},{"name":"Unidad de Resistencia a Antibi\u00f3ticos y Virulencia Bacteriana asociada al Consejo Superior de Investigaciones Cient\u00edficas (CSIC), 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/nrmicro2761","article-title":"The rise of the Enterococcus: Beyond vancomycin resistance","volume":"10","author":"Arias","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","first-page":"788","article-title":"Global Emergence and Dissemination of Enterococci as Nosocomial Pathogens: Attack of the Clones?","volume":"7","author":"Rogers","year":"2016","journal-title":"Front. 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