{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:16:57Z","timestamp":1789791417554,"version":"4.0.1"},"reference-count":27,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dutch Ministry of Agriculture, Nature and Food Quality of the Netherlands","award":["WOT-01-001-003"],"award-info":[{"award-number":["WOT-01-001-003"]}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020","doi-asserted-by":"publisher","award":["727715"],"award-info":[{"award-number":["727715"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Wesselsbron virus (WSLV) is a neglected, mosquito-borne flavivirus that is endemic to the African continent. The virus is teratogenic to ruminants and causes a self-limiting febrile illness in humans. Wesselsbron disease manifests with similar clinical signs and occurs in the same areas under the same climatic conditions as Rift Valley fever, which is therefore included in the differential diagnosis. Although the gross pathology of WSLV infection in pregnant ewes is reported in literature, the pathogenesis that leads to stillbirths, congenital malformations and abortion has remained undescribed. In the present study, pregnant ewes were inoculated with WSLV and subjected to detailed clinical- and histopathology 8 days later. The virus was mainly detected in foetal trophoblasts of the placenta and in neural progenitor cells, differentiated neurons, oligodendrocytes, microglia and astrocytes. Our study demonstrates that WSLV efficiently crosses the maternal\u2013foetal interface and is highly neuroinvasive in the ovine foetus.<\/jats:p>","DOI":"10.3390\/pathogens9050373","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T02:55:41Z","timestamp":1589424941000},"page":"373","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Early Pathogenesis of Wesselsbron Disease in Pregnant Ewes"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6851-3636","authenticated-orcid":false,"given":"Judith","family":"Oymans","sequence":"first","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Houtribweg 39, 8221 RA Lelystad, The Netherlands"},{"name":"Laboratory of Virology, Wageningen University &amp; Research, P.O. Box 16, 6700 AA Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucien","family":"van Keulen","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Houtribweg 39, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9790-2438","authenticated-orcid":false,"given":"Paul","family":"Wichgers Schreur","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Houtribweg 39, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0329-0176","authenticated-orcid":false,"given":"Jeroen","family":"Kortekaas","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Houtribweg 39, 8221 RA Lelystad, The Netherlands"},{"name":"Laboratory of Virology, Wageningen University &amp; Research, P.O. Box 16, 6700 AA Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/ncpneuro0176","article-title":"West Nile virus meningoencephalitis","volume":"2","author":"Debiasi","year":"2006","journal-title":"Nat. Clin. Pract. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.3390\/v5112825","article-title":"Large human outbreak of West Nile virus infection in north-eastern Italy in 2012","volume":"5","author":"Barzon","year":"2013","journal-title":"Viruses"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.antiviral.2018.12.005","article-title":"West Nile virus epizootic in Germany, 2018","volume":"162","author":"Ziegler","year":"2019","journal-title":"Antivir. 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