{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:52:36Z","timestamp":1790729556327,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Bovine leptospirosis is a bacterial zoonotic disease caused by pathogenic Leptospira spp. The pathology and epidemiology of this infection are influenced by the numerous existing serovars and their adaptation to specific hosts. Infections by host-maintained serovars such as Hardjo are well documented, unlike those from the incidental ones. In July 2014, an emerging phenomenon of an increased incidence of icteric abortions associated with leptospiral infection occurred in southern Belgium. First-line serological analyses targeting cattle-adapted serovars failed at initial diagnosis. This study provides a comprehensive description of laboratory findings\u2014at the level of necropsy, serology and molecular diagnosis\u2014regarding icteric and non-icteric abortions (n = 116) recorded during this time (years 2014\u20132015) and associated with incidental infection by serovars such as Grippotyphosa, Australis and Icterohaemorrhagiae. Based on these tests, a diagnostic pathway is proposed for these types of infection in cattle to establish an affordable but accurate diagnosis in the future. These investigations add insights into the understanding of the pathogenesis of bovine leptospirosis associated with serovars classically described as non-maintenance.<\/jats:p>","DOI":"10.3390\/pathogens9060413","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T05:35:52Z","timestamp":1590557752000},"page":"413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2729-2499","authenticated-orcid":false,"given":"Fabien","family":"Gr\u00e9goire","sequence":"first","affiliation":[{"name":"Association R\u00e9gionale de Sant\u00e9 et d\u2019Identification Animales (ARSIA), B-5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00efssa","family":"Bakinahe","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Animals Unit, Veterinary Bacteriology, Sciensano, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Petitjean","sequence":"additional","affiliation":[{"name":"Association R\u00e9gionale de Sant\u00e9 et d\u2019Identification Animales (ARSIA), B-5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Boarbi","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Animals Unit, Veterinary Bacteriology, Sciensano, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Delooz","sequence":"additional","affiliation":[{"name":"Association R\u00e9gionale de Sant\u00e9 et d\u2019Identification Animales (ARSIA), B-5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Fretin","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Animals Unit, Veterinary Bacteriology, Sciensano, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Saulmont","sequence":"additional","affiliation":[{"name":"Association R\u00e9gionale de Sant\u00e9 et d\u2019Identification Animales (ARSIA), B-5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7191-9191","authenticated-orcid":false,"given":"Marcella","family":"Mori","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Animals Unit, Veterinary Bacteriology, Sciensano, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vincent, A.T., Schiettekatte, O., Goarant, C., Neela, V.K., Bernet, E., Thibeaux, R., Ismail, N., Khalid, M.K.N.M., Amran, F., and Masuzawa, T. 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