{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T14:13:23Z","timestamp":1792332803212,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T00:00:00Z","timestamp":1591747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The intranasal administration of Naegleria fowleri lysates plus cholera toxin (CT) increases protection against N. fowleri meningoencephalitis in mice, suggesting that humoral immune response mediated by antibodies is crucial to induce protection against the infection. In the present study, we applied a protein analysis to detect and identify immunogenic antigens from N. fowleri, which might be responsible for such protection. A Western blot assay of N. fowleri polypeptides was performed using the serum and nasal washes from mice immunized with N. fowleri lysates, either alone or with CT after one, two, three, or four weekly immunizations and challenged with trophozoites of N. fowleri. Immunized mice with N. fowleri plus CT, after four doses, had the highest survival rate (100%). Nasal or sera IgA and IgG antibody response was progressively stronger as the number of immunizations was increased, and that response was mainly directed to 250, 100, 70, 50, 37, and 19 kDa polypeptide bands, especially in the third and fourth immunization. Peptides present in these immunogenic bands were matched by nano-LC\u2013ESI-MSMS with different proteins, which could serve as candidates for a vaccine against N. fowleri infection.<\/jats:p>","DOI":"10.3390\/pathogens9060460","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T07:13:16Z","timestamp":1591773196000},"page":"460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of Immunogenic Antigens of Naegleria fowleri Adjuvanted by Cholera Toxin"],"prefix":"10.3390","volume":"9","author":[{"given":"Sa\u00fal","family":"Rojas-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Guti\u00e9rrez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1362-4205","authenticated-orcid":false,"given":"Diego Alexander","family":"Rojas-Ortega","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de Mexico 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Bonilla-Lemus","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Grupo CyMA, Unidad de Investigaci\u00f3n Interdisciplinaria en Ciencias de la Salud y la Educaci\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, UNAM FES Iztacala, Los Reyes Iztacala, Tlalnepantla C.P. 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5273-3167","authenticated-orcid":false,"given":"Arturo","family":"Contis-Montes de Oca","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Grupo CyMA, Unidad de Investigaci\u00f3n Interdisciplinaria en Ciencias de la Salud y la Educaci\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, UNAM FES Iztacala, Los Reyes Iztacala, Tlalnepantla C.P. 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Herrera-D\u00edaz","sequence":"additional","affiliation":[{"name":"Unidad de Servicios de Apoyo a la Investigaci\u00f3n y la Industria, Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de Mexico C.P. 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"L\u00f3pez-Reyes","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de la Ciudad de M\u00e9xico (UACM), Plantel Cuautepec, Av. La Corona 320, Col. Loma la Palma, Alcald\u00eda Gustavo A. Madero, Ciudad de Mexico C.P. 07160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6290-3393","authenticated-orcid":false,"given":"Mar\u00eda Maricela","family":"Carrasco-Y\u00e9pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Grupo CyMA, Unidad de Investigaci\u00f3n Interdisciplinaria en Ciencias de la Salud y la Educaci\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, UNAM FES Iztacala, Los Reyes Iztacala, Tlalnepantla C.P. 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.4103\/0972-5229.94432","article-title":"A rare case of survival from primary amebic meningoencephalitis","volume":"16","author":"Gautam","year":"2012","journal-title":"Indian J. Crit. 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