{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:44:13Z","timestamp":1791251053726,"version":"4.1.0"},"reference-count":82,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T00:00:00Z","timestamp":1592438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["IZSVE RC 16\/12, IZSVE 558 RC 05\/2017 and IZSLER PRC2017016"],"award-info":[{"award-number":["IZSVE RC 16\/12, IZSVE 558 RC 05\/2017 and IZSLER PRC2017016"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Leptospirosis in dogs has been largely described worldwide, and epidemiological studies have been mainly based on serological data. This study aims to detect and genotype leptospires affecting symptomatic dogs in Northeast Italy between 2013 and 2019. Overall, 1631 dogs were tested using real-time PCR, and leptospires from 193 dogs were subjected to Multilocus Sequence Typing and a Multiple Loci Variable-number Tandem Repeat Analysis. Leptospires were successfully isolated from 15 symptomatic dogs. Six distinct Sequence Types (STs) were found for 135 leptospires, with 3 STs characterizing Leptospira interrogans (ST17, ST198 and ST24), 2 STs characterizing Leptospira kirschneri (ST117 and ST289) and 1 ST characterizing Leptospira borgpetersenii (ST155), revealing the circulation of the serogroups Icterohaemorrhagiae, Australis, Sejroe and Pomona. The Multiple Loci Variable-number Tandem Repeat Analysis of 17 samples did not result in any additional discrimination. Genotypes were compared with those of strains present in the historical internal database, and possible transmission chains were identified from rat, mouse, hedgehog and pig. This work highlights the importance of molecular methods in revealing and identifying circulating Leptospira strains, and it also encourages the evaluation of the ability of commercially available vaccines to reduce the disease burden among dogs.<\/jats:p>","DOI":"10.3390\/pathogens9060484","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T11:00:56Z","timestamp":1592478056000},"page":"484","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Detection of New Leptospira Genotypes Infecting Symptomatic Dogs: Is a New Vaccine Formulation Needed?"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1082-982X","authenticated-orcid":false,"given":"Cristina","family":"Bertasio","sequence":"first","affiliation":[{"name":"National Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna \u201cBruno Ubertini\u201d, 25121 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Beatrice","family":"Boniotti","sequence":"additional","affiliation":[{"name":"National Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna \u201cBruno Ubertini\u201d, 25121 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-9024","authenticated-orcid":false,"given":"Laura","family":"Lucchese","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Ceglie","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bellinati","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4019-2855","authenticated-orcid":false,"given":"Matteo","family":"Mazzucato","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1935-5146","authenticated-orcid":false,"given":"Tommaso","family":"Furlanello","sequence":"additional","affiliation":[{"name":"\u201cSan Marco\u201d Veterinary Clinica and Laboratory, Via dell\u2019 Industria, 35030 Veggiano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9918-2497","authenticated-orcid":false,"given":"Mario","family":"D\u2019Incau","sequence":"additional","affiliation":[{"name":"National Reference Centre for Animal Leptospirosis, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna \u201cBruno Ubertini\u201d, 25121 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2104-4531","authenticated-orcid":false,"given":"Alda","family":"Natale","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell\u2019Universit\u00e0, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/jsap.12328","article-title":"European consensus statement on leptospirosis in dogs and cats","volume":"56","author":"Schuller","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_2","first-page":"4271","article-title":"Appearance in Holland of Leptospira differing from Weil Strain","volume":"77","author":"Klarenbeek","year":"1933","journal-title":"Ned. Tijdschr. Geneeskd."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/zph.12236","article-title":"Meta-Analyses of Factors Associated with Leptospirosis in Domestic Dogs","volume":"63","author":"Monti","year":"2016","journal-title":"Zoonoses Public Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1751-0813.2008.00324.x","article-title":"Cross-sectional study of canine leptospirosis in animal shelter populations in mainland Australia","volume":"86","author":"Zwijnenberg","year":"2008","journal-title":"Aust. Vet. J."},{"key":"ref_5","first-page":"280","article-title":"Serological survey of canine leptospirosis in Southern China","volume":"32","author":"Shi","year":"2012","journal-title":"Pak. Vet. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100104","DOI":"10.1016\/j.onehlt.2019.100104","article-title":"Frequency of anti-Leptospira spp. antibodies in dogs and wild small mammals from rural properties and conservation units in southern Brazil","volume":"8","author":"Guedes","year":"2019","journal-title":"One Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"103833","DOI":"10.1016\/j.micpath.2019.103833","article-title":"Serological evidence of leptospirosis in Iran; A systematic review and meta-analysis","volume":"138","author":"Khalili","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1111\/j.1863-2378.2008.01137.x","article-title":"Serological survey for antibodies to Leptospira in dogs and raccoons in Washington State","volume":"55","author":"Davis","year":"2008","journal-title":"Zoonoses Public Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e02373","DOI":"10.1016\/j.heliyon.2019.e02373","article-title":"Leptospira seroprevalence in owned dogs from Spain","volume":"5","author":"Vila","year":"2019","journal-title":"Heliyon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5455\/vetworld.2013.42-44","article-title":"Canine leptospirosis\u2014A seroprevalence study from Kerala, India","volume":"6","author":"Ambily","year":"2013","journal-title":"Vet. World"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1748-5827.2002.tb00048.x","article-title":"Serological survey of leptospiral infection in kennelled dogs in Italy","volume":"43","author":"Scanziani","year":"2002","journal-title":"J. Small Anim. Pract."},{"key":"ref_12","first-page":"129","article-title":"Serological surveillance of Leptospirosis in Italy: Two\u2013year national data (2010-2011)","volume":"52","author":"Tagliabue","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.cimid.2019.04.001","article-title":"Epidemiology of leptospirosis in North-Central Italy: Fifteen years of serological data (2002\u20132016)","volume":"65","author":"Bertelloni","year":"2019","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1748-5827.1960.tb06099.x","article-title":"The Evaluation of a Combined L.canicola and L.icterohaemorrhagiae Vaccine on Hamsters and Dogs","volume":"1","author":"Jull","year":"1960","journal-title":"J. Small Anim. Pract."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vetmic.2006.04.005","article-title":"Canine leptospirosis-Do we have a problem?","volume":"117","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1939-1676.2010.0654.x","article-title":"2010 ACVIM Small Animal Consensus Statement on Leptospirosis: Diagnosis, Epidemiology, Treatment, and Prevention","volume":"25","author":"Sykes","year":"2011","journal-title":"J. Vet. Intern. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1136\/vr.c4965","article-title":"Control of canine leptospirosis in Europe: Time for a change?","volume":"167","author":"Ellis","year":"2010","journal-title":"Vet. Rec."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1089\/vbz.2012.1121","article-title":"Distribution of Leptospira Serogroups in Dogs from Berlin, Germany","volume":"13","author":"Luge","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.tvjl.2012.10.002","article-title":"Prevalence of the Leptospira serovars bratislava, grippotyphosa, mozdok and pomona in French dogs","volume":"196","author":"Renaud","year":"2013","journal-title":"Vet. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1136\/vr.101100","article-title":"A novel tetravalent Leptospira bacterin protects against infection and shedding following challenge in dogs","volume":"172","author":"Klaasen","year":"2013","journal-title":"Vet. Rec."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.vetimm.2013.08.002","article-title":"A new tetravalent canine leptospirosis vaccine provides at least 12 months immunity against infection","volume":"158","author":"Klaasen","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_23","unstructured":"(2018). Leptospirosis. OIE Terrestrial Manual, World Organization for Animal Health."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s40813-018-0086-5","article-title":"Passive surveillance of Leptospira infection in swine in Germany","volume":"4","author":"Tschentscher","year":"2018","journal-title":"Porc. Health Manag."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1086\/346208","article-title":"Usefulness of Serologic Analysis as a Predictor of the Infecting Serovar in Patients with Severe Leptospirosis","volume":"36","author":"Levett","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/jvim.12337","article-title":"Vaccine-Associated Leptospira Antibodies in Client-Owned Dogs","volume":"28","author":"Martin","year":"2014","journal-title":"J. Vet. Intern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1099\/jmm.0.45860-0","article-title":"Detection of pathogenic leptospires by real-time quantitative PCR","volume":"54","author":"Levett","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Smythe, L.D., Smith, I.L., Smith, G.A., Dohnt, M.F., Symonds, M.L., Barnett, L.J., and McKay, D.B. (2002). A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp.. BMC Infect. Dis., 2.","DOI":"10.1186\/1471-2334-2-13"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.femsle.2005.06.011","article-title":"A rapid and quantitative method for the detection of Leptospira species in human leptospirosis","volume":"249","author":"Merien","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ahmed, A., Engelberts, M.F.M., Boer, K.R., Ahmed, N., and Hartskeerl, R.A. (2009). Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0007093"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Slack, A.T., Symonds, M.L., Dohnt, M.F., and Smythe, L.D. (2006). Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol., 6.","DOI":"10.1186\/1471-2180-6-95"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1128\/JCM.02452-10","article-title":"Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences","volume":"49","author":"Bourhy","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.2460\/javma.2003.222.1230","article-title":"Comparison of polymerase chain reaction assay, bacteriologic culture, and serologic testing in assessment of prevalence of urinary shedding of leptospires in dogs","volume":"222","author":"Harkin","year":"2003","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1939-1676.2011.00852.x","article-title":"Effects of recent Leptospira vaccination on whole blood real-time PCR testing in healthy client-owned dogs","volume":"26","author":"Midence","year":"2012","journal-title":"J. Vet. Intern. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Boonsilp, S., Thaipadungpanit, J., Amornchai, P., Wuthiekanun, V., Bailey, M.S., Holden, M.T.G., Zhang, C., Jiang, X., Koizumi, N., and Taylor, K. (2013). A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0001954"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3954","DOI":"10.1128\/JCM.00336-06","article-title":"Application of Multilocus Variable-Number Tandem-Repeat Analysis for Molecular Typing of the Agent of Leptospirosis","volume":"44","author":"Gurianova","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Weiss, S., Menezes, A., Woods, K., Chanthongthip, A., Dittrich, S., Opoku-Boateng, A., Simuli, M., and Chalke, V. (2016). An Extended Multilocus Sequence Typing (MLST) Scheme for Rapid Direct Typing of Leptospira from Clinical Samples. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004996"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0378-1135(03)00152-4","article-title":"Duration of immunity in dogs vaccinated against leptospirosis with a bivalent inactivated vaccine","volume":"95","author":"Klaasen","year":"2003","journal-title":"Vet. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1016\/j.vaccine.2013.05.043","article-title":"Duration of immunity of a multivalent (DHPPi\/L4R) canine vaccine against four Leptospira serovars","volume":"31","author":"Wilson","year":"2013","journal-title":"Vaccine"},{"key":"ref_41","unstructured":"Boniotti, M.B., Gelmini, L., Carra, E., Figarolli, B.M., D\u2019incau, M., and Tagliabue, S. (2013, January 7\u201311). Leptospira Interrogans Serogroup Australis In Hedgehog In Northern Italy. Proocedings of the International Leptospirosis Society of the Conference, Fukuoka, Japan."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Bertasio, C., Papetti, A., Scaltriti, E., Tagliabue, S., D\u2019Incau, M., and Boniotti, M.B. (2020). Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9050332"},{"key":"ref_43","first-page":"43","article-title":"Leptospirosis in a dog caused by Leptospira interrogans serotype pomona","volume":"41","author":"Modric","year":"1987","journal-title":"Vet. Glas."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"719","DOI":"10.3906\/vet-1505-18","article-title":"Seroepidemiological survey of leptospiral infection in stray dogs in Serbia","volume":"39","year":"2015","journal-title":"Turkish J. Vet. Anim. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1136\/vr.153.5.146","article-title":"Prevalence of Leptospira species among farmed and domestic animals in Greece","volume":"153","author":"Burriel","year":"2003","journal-title":"Vet. Rec."},{"key":"ref_46","first-page":"383","article-title":"Epidemiology of leptospirosis: Observations on serological data obtained by a \u201cdiagnostic laboratory for leptospirosis\u201d from 1995 to 2001","volume":"26","author":"Cerri","year":"2003","journal-title":"New Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Felzemburgh, R.D.M., Ribeiro, G.S., Costa, F., Reis, R.B., Hagan, J.E., Melendez, A.X.T.O., Fraga, D., Santana, F.S., Mohr, S., and dos Santos, B.L. (2014). Prospective Study of Leptospirosis Transmission in an Urban Slum Community: Role of Poor Environment in Repeated Exposures to the Leptospira Agent. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002927"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.bjm.2016.12.010","article-title":"Human leptospirosis: Occurrence of serovars of Leptospira spp. in the state of Minas Gerais, Brazil, from 2008 to 2012","volume":"48","author":"Oliveira","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1017\/S0950268809990902","article-title":"Serovars of Leptospira isolated from dogs and rodents","volume":"138","author":"Suepaul","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Boey, K., Shiokawa, K., and Rajeev, S. (2019). Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007499"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1051\/vetres:2006043","article-title":"Use of a case-control study and geographic information systems to determine environmental and demographic risk factors for canine leptospirosis","volume":"38","author":"Ghneim","year":"2007","journal-title":"Vet. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7242","DOI":"10.3390\/ijerph110707242","article-title":"Increasing incidence of canine leptospirosis in Switzerland","volume":"11","author":"Major","year":"2014","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1136\/vr.102916","article-title":"Prevalence of antileptospiral serum antibodies in dogs in Ireland","volume":"177","author":"Schuller","year":"2015","journal-title":"Vet. Rec."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3389\/fcimb.2018.00193","article-title":"Genomic comparison among global isolates of L. interrogans serovars Copenhageni and Icterohaemorrhagiae identified natural genetic variation caused by an indel","volume":"8","author":"Santos","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1177\/1040638714553293","article-title":"Diagnostic features in 10 naturally occurring cases of acute fatal canine leptospirosis","volume":"26","author":"Rissi","year":"2014","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_56","first-page":"1125","article-title":"Serum biochemical changes in dogs with experimental Leptospira interrogans serovar icterohaemorrhagiae infection","volume":"42","author":"CE","year":"1981","journal-title":"Am. J. Vet. Res."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Balboni, A., Zamagni, S., Bertasio, C., Boniotti, M.B., Tro\u00eca, R., Battilani, M., and Dondi, F. (2020). Identification of Serogroups Australis and Icterohaemorrhagiae in Two Dogs with a Severe Form of Acute Leptospirosis in Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9050351"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Jolley, K.A., and Maiden, M.C.J. (2010). BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-595"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/j.1748-5827.1991.tb00526.x","article-title":"A serological and bacteriological survey of leptospiral infection in dogs in Edinburgh and Glasgow","volume":"32","author":"Thrusfield","year":"1991","journal-title":"J. Small Anim. Pract."},{"key":"ref_60","first-page":"11","article-title":"Inchiesta sieroepidemiologica sulla diffusione della leptospirosi ta gli animali domestici ed alcune specie selvatiche","volume":"36","author":"Tagliabue","year":"1995","journal-title":"Sel. Vet."},{"key":"ref_61","first-page":"2","article-title":"Seroprevalence and risk factors of leptospirosis in wild boars (Sus scrofa) in northern Italy","volume":"27","author":"Chiari","year":"2016","journal-title":"Hystrix"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.meegid.2012.09.013","article-title":"Altodouro, a new Leptospira serovar of the Pomona serogroup isolated from rodents in northern Portugal","volume":"13","author":"Arent","year":"2013","journal-title":"Infect. Genet. Evol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.rvsc.2003.12.003","article-title":"Microbiological and serological study of leptospirosis in horses at slaughter: First isolations","volume":"76","author":"Rocha","year":"2004","journal-title":"Res. Vet. Sci."},{"key":"ref_64","first-page":"602","article-title":"Isolation of Leptospira interrogans serovar mozdok from aborted swine fetuses in Portugal","volume":"126","author":"Rocha","year":"1990","journal-title":"Vet. Rec."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.actatropica.2013.12.009","article-title":"Epizootiological survey of small mammals as Leptospira spp. reservoirs in Eastern Croatia","volume":"131","author":"Majetic","year":"2014","journal-title":"Acta Trop."},{"key":"ref_66","first-page":"343","article-title":"Outbreak of Leptospira interrogans serovar mozdok in sows in Dorset, England","volume":"43","author":"Pritchard","year":"1987","journal-title":"Isr. J. Vet. Med."},{"key":"ref_67","first-page":"123","article-title":"Reproductive failure in sows associated with Leptospira Mozdok from a wildlife source","volume":"54","author":"Barlow","year":"2004","journal-title":"Pig J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1136\/vr.115.24.623","article-title":"Possible role of leptospires of the Pomona serogroup in sporadic bovine abortion in the south west of England","volume":"115","author":"Hathaway","year":"1984","journal-title":"Vet. Rec."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4269\/ajtmh.15-0505","article-title":"Infection with Leptospira kirschneri Serovar Mozdok: First Report from the Southern Hemisphere","volume":"94","author":"Felix","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.2460\/ajvr.2005.66.1816","article-title":"Experimental canine leptospirosis caused by Leptospira interrogans serovars pomona and bratislava","volume":"66","author":"Greenlee","year":"2005","journal-title":"Am. J. Vet. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/j.1939-1676.2006.tb02886.x","article-title":"Influence of infecting serogroup on clinical features of leptospirosis in dogs","volume":"20","author":"Goldstein","year":"2006","journal-title":"J. Vet. Intern. Med."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0940-2993(00)80029-9","article-title":"Clinical, microbiological and pathological observations in laboratory beagle dogs infected with leptospires of the serogroup sejroe","volume":"52","author":"Brem","year":"2000","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1258\/002367795781088261","article-title":"Leptospira interrogans serovar sejroe infection in a group of laboratory dogs","volume":"29","author":"Scanziani","year":"1995","journal-title":"Lab. Anim."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Miotto, B.A., Tozzi, B.F., de Souza Penteado, M., Guilloux, A.G.A., Moreno, L.Z., Heinemann, M.B., Moreno, A.M., Lilenbaum, W., and Hagiwara, M.K. (2018). Diagnosis of acute canine leptospirosis using multiple laboratory tests and characterization of the isolated strains. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1547-4"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2016.06.007","article-title":"Molecular and serological characterization of the first Leptospira santarosai strain isolated from a dog","volume":"162","author":"Miotto","year":"2016","journal-title":"Acta Trop."},{"key":"ref_76","first-page":"259","article-title":"Leptospirosis in dogs: A retrospective study of seven clinical cases in Belgium","volume":"77","author":"Claus","year":"2008","journal-title":"Vlaams Diergeneeskd. Tijdschr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"343","DOI":"10.7589\/0090-3558-8.4.343","article-title":"Leptospirosis in Danish wild mammals","volume":"8","author":"Fennestad","year":"1972","journal-title":"J. Wildl. Dis."},{"key":"ref_78","first-page":"177","article-title":"Small terrestrial mammals as reservoirs of leptospires in Sava valley (Croatia)","volume":"29","author":"Aleraj","year":"2005","journal-title":"Folia Parasitol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/BF00572121","article-title":"Muskrats as carriers of pathogenic leptospires in The Netherlands","volume":"61","author":"Steinen","year":"1992","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_80","first-page":"15","article-title":"Classification of serovars of the icterohaemorrhagiae serogroup by monoclonal antibodies","volume":"44","author":"Korver","year":"1988","journal-title":"Isr. J. Vet."},{"key":"ref_81","first-page":"412","article-title":"Comparative classification of Leptospira serovars of the Pomona group by monoclonal antibodies and restriction-endonuclease analysis","volume":"266","author":"Terpstra","year":"1987","journal-title":"Zentralbl. Bakteriol. Mikrobiol. Hyg. A."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/j.1574-6968.1989.tb02441.x","article-title":"First human isolate of Leptospira interrogans as serovar bratislava in Italy","volume":"47","author":"Cinco","year":"1989","journal-title":"FEMS Microbiol. Lett."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/6\/484\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:40:21Z","timestamp":1760175621000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/6\/484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,18]]},"references-count":82,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["pathogens9060484"],"URL":"https:\/\/doi.org\/10.3390\/pathogens9060484","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,18]]}}}