{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:24:28Z","timestamp":1780075468189,"version":"3.54.0"},"reference-count":79,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Feline infectious peritonitis (FIP) is a coronavirus-induced disease of cats, in which the immune system is known to play a crucial, but complex, role in the pathogenesis. This role is still incompletely understood, with involvement of both host and viral factors. To evaluate differential gene expression and pathway involvement in feline coronavirus (FCoV) infection and FIP, we applied next-generation RNA-sequencing of the mesenteric lymph nodes from cats with naturally-acquired FIP, as well as those with systemic FCoV infection without FIP, and those with neither. Viral infection was associated with upregulation of viral defenses regardless of the disease state, but to a greater degree in FIP. FIP was associated with higher pro-inflammatory pathway enrichment, whilst non-FIP FCoV-positive cats showed lower enrichment of humoral immunity pathways, below that of uninfected cats in the case of immunoglobulin production pathways. This host response is presumed to be protective. In FIP, downregulation of T cell-related processes was observed, which did not occur in non-FIP FCoV-positive cats. These results emphasize the importance of the host\u2019s immune balance in determining the outcome of the FCoV infection.<\/jats:p>","DOI":"10.3390\/pathogens9070524","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T11:17:17Z","timestamp":1593429437000},"page":"524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Effect of Natural Feline Coronavirus Infection on the Host Immune Response: A Whole-Transcriptome Analysis of the Mesenteric Lymph Nodes in Cats with and without Feline Infectious Peritonitis"],"prefix":"10.3390","volume":"9","author":[{"given":"Alexandra J.","family":"Malbon","sequence":"first","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"},{"name":"Centre for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Russo","sequence":"additional","affiliation":[{"name":"Functional Genomics Center Zurich, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Burgener","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"},{"name":"Centre for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6293-6662","authenticated-orcid":false,"given":"Emi N.","family":"Barker","sequence":"additional","affiliation":[{"name":"Langford Clinical Veterinary Service, University of Bristol, Langford, Bristol BS40 5DU, UK"},{"name":"Bristol Veterinary School, University of Bristol, Langford, Bristol BS40 5DU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3609-2416","authenticated-orcid":false,"given":"Marina L.","family":"Meli","sequence":"additional","affiliation":[{"name":"Centre for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"},{"name":"Clinical Laboratory, Department of Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4059-1402","authenticated-orcid":false,"given":"S\u00e9verine","family":"Tasker","sequence":"additional","affiliation":[{"name":"Bristol Veterinary School, University of Bristol, Langford, Bristol BS40 5DU, UK"},{"name":"The Linnaeus Group, Shirley, Solihull B90 1BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7289-3459","authenticated-orcid":false,"given":"Anja","family":"Kipar","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"ref_1","unstructured":"King, A.M.Q., Adams, M.J., Carstens, E.B., and Lefkowitz, E.J. (2012). Family-Coronaviridae. Virus Taxonomy, Elsevier."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/s41591-020-0820-9","article-title":"The proximal origin of SARS-CoV-2","volume":"26","author":"Andersen","year":"2020","journal-title":"Nat. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1177\/0300985814522077","article-title":"Feline infectious peritonitis: Still an enigma?","volume":"51","author":"Kipar","year":"2014","journal-title":"Vet. Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jfms.2003.08.007","article-title":"High viral loads despite absence of clinical and pathological findings in cats experimentally infected with feline coronavirus (FCoV) type I and in naturally FCoV-infected cats","volume":"6","author":"Meli","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"429","DOI":"10.2460\/ajvr.1995.56.04.429","article-title":"Risk of feline infectious peritonitis in cats naturally infected with feline coronavirus","volume":"56","author":"Addie","year":"1995","journal-title":"Am. J. Vet. Res."},{"key":"ref_6","first-page":"868","article-title":"Immunologic phenomena in the effusive form of feline infectious peritonitis","volume":"41","author":"Pedersen","year":"1980","journal-title":"Am. J. Vet. Res."},{"key":"ref_7","first-page":"157","article-title":"Some important disorders of cats","volume":"53","author":"Holzworth","year":"1963","journal-title":"Cornell Vet."},{"key":"ref_8","first-page":"32","article-title":"Sulla pleurite infettiva del gatto","volume":"21","author":"Bonaduce","year":"1942","journal-title":"Nuova Vet."},{"key":"ref_9","first-page":"255","article-title":"Feline infectious peritonitis","volume":"3","author":"Wolfe","year":"1966","journal-title":"Pathol. Vet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.3201\/eid1907.121094","article-title":"Mutation in spike protein cleavage site and pathogenesis of feline coronavirus","volume":"19","author":"Licitra","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.rvsc.2014.07.016","article-title":"Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats","volume":"97","author":"Borschensky","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jcpa.2018.11.001","article-title":"Inflammatory mediators in the mesenteric lymph nodes, site of a possible intermediate phase in the immune response to feline coronavirus and the pathogenesis of feline infectious peritonitis?","volume":"166","author":"Malbon","year":"2019","journal-title":"J. Comp. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-017-0467-9","article-title":"Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis","volume":"48","author":"Barker","year":"2017","journal-title":"Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/1297-9716-45-49","article-title":"Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis","volume":"45","author":"Porter","year":"2014","journal-title":"Vet. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.3201\/eid1807.120143","article-title":"Spike protein fusion peptide and feline coronavirus virulence","volume":"18","author":"Chang","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-2438-7_1","article-title":"Coronaviruses: An overview of their replication and pathogenesis","volume":"1282","author":"Fehr","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"13910","DOI":"10.1073\/pnas.96.24.13910","article-title":"Mutation rates among RNA viruses","volume":"96","author":"Drake","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.vetmic.2008.06.007","article-title":"An update on canine coronaviruses: Viral evolution and pathobiology","volume":"132","author":"Decaro","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_19","first-page":"14","article-title":"The inheritance of susceptibility to feline infectious peritonitis in purebred catteries","volume":"24","author":"Foley","year":"1996","journal-title":"Feline Pract."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.virusres.2013.04.006","article-title":"Genetic susceptibility to feline infectious peritonitis in Birman cats","volume":"175","author":"Golovko","year":"2013","journal-title":"Virus Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jfms.2005.04.003","article-title":"Prevalence of feline infectious peritonitis in specific cat breeds","volume":"8","author":"Radzai","year":"2006","journal-title":"J. Feline Med. Surg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/978-1-4684-1280-2_69","article-title":"Virologic and immunologic aspects of feline infectious peritonitis virus infection","volume":"218","author":"Pedersen","year":"1987","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.vetimm.2014.09.001","article-title":"The influence of age and genetics on natural resistance to experimentally induced feline infectious peritonitis","volume":"162","author":"Pedersen","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1128\/JVI.01568-09","article-title":"Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage","volume":"84","author":"Tekes","year":"2010","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jfms.2005.07.002","article-title":"Natural FCoV infection: Cats with FIP exhibit significantly higher viral loads than healthy infected cats","volume":"8","author":"Kipar","year":"2006","journal-title":"J. Feline Med. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1007\/s00705-005-0598-6","article-title":"Replication of feline coronaviruses in peripheral blood monocytes","volume":"150","author":"Dewerchin","year":"2005","journal-title":"Arch. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1128\/jvi.63.1.436-440.1989","article-title":"Intrinsic resistance of feline peritoneal macrophages to coronavirus infection correlates with in vivo virulence","volume":"63","author":"Stoddart","year":"1989","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1354\/vp.42-3-321","article-title":"Morphologic features and development of granulomatous vasculitis in feline infectious peritonitis","volume":"42","author":"Kipar","year":"2005","journal-title":"Vet. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.tvjl.2014.04.016","article-title":"An update on feline infectious peritonitis: Diagnostics and therapeutics","volume":"201","author":"Pedersen","year":"2014","journal-title":"Vet. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.vetimm.2006.02.004","article-title":"Natural feline coronavirus infection: Differences in cytokine patterns in association with the outcome of infection","volume":"112","author":"Kipar","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.jfms.2006.05.002","article-title":"Whole blood cytokine profiles in cats infected by feline coronavirus and healthy non-FCoV infected specific pathogen-free cats","volume":"8","author":"Gelain","year":"2006","journal-title":"J. Feline Med. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jfms.2003.08.009","article-title":"Disease outcome and cytokine responses in cats immunized with an avirulent feline infectious peritonitis virus (FIPV)-UCD1 and challenge-exposed with virulent FIPV-UCD8","volume":"6","author":"Kiss","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0165-2427(98)00156-1","article-title":"Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)","volume":"65","author":"Caney","year":"1998","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Malbon, A.J., Fonfara, S., Meli, M.L., Hahn, S., Egberink, H., and Kipar, A. (2019). Feline infectious peritonitis as a systemic inflammatory disease: Contribution of liver and heart to the pathogenesis. Viruses, 11.","DOI":"10.3390\/v11121144"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3180","DOI":"10.1128\/jcm.34.12.3180-3184.1996","article-title":"Two related strains of feline infectious peritonitis virus isolated from immunocompromised cats infected with a feline enteric coronavirus","volume":"34","author":"Poland","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kedward-Dixon, H., Barker, E.N., Tasker, S., Kipar, A., and Helps, C.R. (2019). Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis. J. Feline Med. Surg., 22.","DOI":"10.1177\/1098612X19865637"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetimm.2016.01.002","article-title":"Natural resistance to experimental feline infectious peritonitis virus infection is decreased rather than increased by positive genetic selection","volume":"171","author":"Pedersen","year":"2016","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1084\/jem.20060227","article-title":"Mesenteric lymph nodes at the center of immune anatomy","volume":"203","author":"Macpherson","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1053\/jcpa.2001.0501","article-title":"A comparison of lymphatic tissues from cats with spontaneous feline infectious peritonitis (FIP), cats with FIP virus infection but no FIP, and cats with no infection","volume":"125","author":"Kipar","year":"2001","journal-title":"J. Comp. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0378-1135(99)00101-7","article-title":"Histopathological alterations of lymphatic tissues in cats without feline infectious peritonitis after long-term exposure to FIP virus","volume":"69","author":"Kipar","year":"1999","journal-title":"Vet. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2003.08.010","article-title":"In vivo cytokine response to experimental feline infectious peritonitis virus infection","volume":"97","author":"Dean","year":"2003","journal-title":"Vet. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13567-018-0578-y","article-title":"Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq","volume":"49","author":"Watanabe","year":"2018","journal-title":"Vet. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s10495-015-1172-7","article-title":"Apoptosis transcriptional mechanism of feline infectious peritonitis virus infected cells","volume":"20","author":"Shuid","year":"2015","journal-title":"Apoptosis"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/978-1-4939-2438-7_20","article-title":"Transcriptome analysis of feline infectious peritonitis virus infection","volume":"1282","author":"Mehrbod","year":"2015","journal-title":"Coronaviruses Methods Protoc. Methods Mol. Biol."},{"key":"ref_45","first-page":"229","article-title":"Attempted immunization of cats against feline infectious peritonitis, using avirulent live virus or sublethal amounts of virulent virus","volume":"44","author":"Pedersen","year":"1983","journal-title":"Am. J. Vet. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1128\/jvi.66.11.6695-6705.1992","article-title":"Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages","volume":"66","author":"Corapi","year":"1992","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1292\/jvms.70.1315","article-title":"Antibody-dependent enhancement occurs upon re-infection with the identical serotype virus in feline infectious peritonitis virus infection","volume":"70","author":"Takano","year":"2008","journal-title":"J. Vet. Med. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1099\/vir.0.83558-0","article-title":"Analysis of the mechanism of antibody-dependent enhancement of feline infectious peritonitis virus infection: Aminopeptidase N is not important and a process of acidification of the endosome is necessary","volume":"89","author":"Takano","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1042\/BC20040058","article-title":"Virus factories: Associations of cell organelles for viral replication and morphogenesis","volume":"97","author":"Novoa","year":"2005","journal-title":"Biol. Cell"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1099\/vir.0.020214-0","article-title":"Sites of feline coronavirus persistence in healthy cats","volume":"91","author":"Kipar","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/nri1604","article-title":"Mechanisms of type-I- and type-II-interferon-mediated signalling","volume":"5","author":"Platanias","year":"2005","journal-title":"Nat. Rev. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.tvjl.2007.06.005","article-title":"The feline acute phase reaction","volume":"177","author":"Paltrinieri","year":"2008","journal-title":"Vet. J."},{"key":"ref_53","first-page":"666","article-title":"Antibody, immune complexes, and complement activity fluctuations in kittens with experimentally induced feline infectious peritonitis","volume":"43","author":"Daha","year":"1982","journal-title":"Am. J. Vet. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1099\/0022-1317-74-4-745","article-title":"Identification of antigenic sites mediating antibody-dependent enhancement of feline infectious peritonitis virus infectivity","volume":"74","author":"Olsen","year":"1993","journal-title":"J. Gen. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: Update on Toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.virusres.2009.01.005","article-title":"SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-kappaB pathway in human monocyte macrophages in vitro","volume":"142","author":"Dosch","year":"2009","journal-title":"Virus Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"8946","DOI":"10.1128\/JVI.01857-08","article-title":"Toll-like receptor 4 deficiency increases disease and mortality after mouse hepatitis virus type 1 infection of susceptible C3H mice","volume":"83","author":"Khanolkar","year":"2009","journal-title":"J. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.coviro.2011.10.006","article-title":"Toll-like receptors: Key players in antiviral immunity","volume":"1","author":"Arpaia","year":"2011","journal-title":"Curr. Opin. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1365-2249.2010.04196.x","article-title":"Recent insights into the role of Toll-like receptors in viral infection","volume":"161","author":"Carty","year":"2010","journal-title":"Clin. Exp. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10456-008-9093-5","article-title":"Convergence and amplification of Toll-like receptor (TLR) and receptor for advanced glycation end products (RAGE) signaling pathways via high mobility group B1 (HMGB1)","volume":"11","author":"Buurman","year":"2008","journal-title":"Angiogenesis"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1128\/JVI.05738-11","article-title":"Sensing of RNA viruses: A review of innate immune receptors involved in recognizing RNA virus invasion","volume":"86","author":"Jensen","year":"2012","journal-title":"J. Virol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Mustaffa-Kamal, F., Liu, H., Pedersen, N.C., and Sparger, E.E. (2019). Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV). BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-1909-6"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nri.2017.75","article-title":"The regulation of immune tolerance by FOXP3","volume":"17","author":"Lu","year":"2017","journal-title":"Nat. Rev. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.vetmic.2013.01.042","article-title":"Suppression of NK cells and regulatory T lymphocytes in cats naturally infected with feline infectious peritonitis virus","volume":"164","author":"Vermeulen","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.rvsc.2014.08.009","article-title":"Relationship between rate of infection and markers of inflammation\/immunity in Holy Birman cats with feline coronavirus","volume":"97","author":"Paltrinieri","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.3389\/fimmu.2017.01187","article-title":"TRIM25 in the Regulation of the Antiviral Innate Immunity","volume":"8","author":"Medrano","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e02143-16","DOI":"10.1128\/JVI.02143-16","article-title":"The Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Inhibits Type I Interferon Production by Interfering with TRIM25-Mediated RIG-I Ubiquitination","volume":"91","author":"Hu","year":"2017","journal-title":"J. Virol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1089\/vim.2011.0099","article-title":"SARS-CoV Regulates Immune Function-Related Gene Expression in Human Monocytic Cells","volume":"25","author":"Hu","year":"2012","journal-title":"Viral Immunol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"van Tol, S., Hage, A., Giraldo, M.I., Bharaj, P., and Rajsbaum, R. (2017). The TRIMendous role of TRIMs in virus-host interactions. Vaccines, 5.","DOI":"10.3390\/vaccines5030023"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0165-2427(98)00158-5","article-title":"Cellular composition, coronavirus antigen expression and production of specific antibodies in lesions in feline infectious peritonitis","volume":"65","author":"Kipar","year":"1998","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0166-0934(98)00129-3","article-title":"One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses","volume":"77","author":"Gut","year":"1999","journal-title":"J. Virol. Methods"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pcbi.1003118","article-title":"Software for Computing and Annotating Genomic Ranges","volume":"9","author":"Lawrence","year":"2013","journal-title":"PLoS Comput. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"ClusterProfiler: An R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics A J. Integr. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_78","unstructured":"(2020, March 21). The Comprehensive R Archive Network. Available online: https:\/\/cran.r-project.org\/web\/packages\/pheatmap\/index.html."},{"key":"ref_79","unstructured":"(2020, June 10). The Comprehensive R Archive Network. Available online: https:\/\/cran.r-project.org\/package=vegan."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/7\/524\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:44:24Z","timestamp":1760175864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/7\/524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,29]]},"references-count":79,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["pathogens9070524"],"URL":"https:\/\/doi.org\/10.3390\/pathogens9070524","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,29]]}}}