{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T20:14:05Z","timestamp":1772050445777,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Feline coronavirus (FCoV), the pathogen for feline infectious peritonitis, is a lethal infectious agent that can cause effusions in the pleural and abdominal cavities in domestic cats. To study the epidemiology of FCoV in Taiwan, 81 FIP-suspected sick cats with effusive specimens were recruited to test for FCoV infection using immunofluorescence staining and reverse transcription-polymerase chain reaction as detection methods, and viral RNAs were recovered from the specimens to conduct genotyping and phylogenetic analysis based on the spike (S) protein gene. The results revealed that a total of 47 (47\/81, 58%) of the sick cats were positive for FCoV in the effusion samples, of which 39 were successfully sequenced and comprised of 21 type I strains, 9 type II strains, and 9 co-infections. The signalment analysis of these sick cats revealed that only the sex of cats showed a significant association (odds ratio = 2.74, 95% confidence interval = 1.06\u20137.07, p = 0.03) with the infection of FCoV, while age and breed showed no association. FCoV-positive cats demonstrated a significantly lower albumin to globulin ratio than negative individuals (p = 0.0004). The partial S gene-based phylogenetic analysis revealed that the type I strains demonstrated genetic diversity forming several clades, while the type II strains were more conserved. This study demonstrates the latest epidemiological status of FCoV infection in the northern part of Taiwan among sick cats and presents comparisons of Taiwan and other countries.<\/jats:p>","DOI":"10.3390\/pathogens9090698","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T09:30:07Z","timestamp":1598347807000},"page":"698","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Detection of Feline Coronavirus in Feline Effusions by Immunofluorescence Staining and Reverse Transcription Polymerase Chain Reaction"],"prefix":"10.3390","volume":"9","author":[{"given":"Yi-Chen","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, National Taiwan University, 10617 Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2448-9050","authenticated-orcid":false,"given":"I-Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, 10617 Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Tan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Taiwan University, 10617 Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0595-3420","authenticated-orcid":false,"given":"Hui-Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Taiwan University, 10617 Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1128\/JVI.02605-06","article-title":"Evolutionary insights into the ecology of coronaviruses","volume":"81","author":"Vijaykrishna","year":"2007","journal-title":"J. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4528","DOI":"10.1128\/JVI.77.8.4528-4538.2003","article-title":"Switching species tropism: An effective way to manipulate the feline coronavirus genome","volume":"77","author":"Haijema","year":"2003","journal-title":"J. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4508","DOI":"10.1128\/JVI.72.5.4508-4514.1998","article-title":"Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus","volume":"72","author":"Herrewegh","year":"1998","journal-title":"J. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/viro.1998.9045","article-title":"Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses","volume":"243","author":"Vennema","year":"1998","journal-title":"Virology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1186\/1743-422X-8-455","article-title":"Prevalence of Korean cats with natural feline coronavirus infections","volume":"8","author":"An","year":"2011","journal-title":"Virol. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1748-5827.1993.tb02591.x","article-title":"Using direct immunofluorescence to detect coronaviruses in peritoneal in peritoneal and pleural effusions","volume":"34","author":"Parodi","year":"1993","journal-title":"J. Small Anim. Pract."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"809480","DOI":"10.4061\/2010\/809480","article-title":"Diagnostic methods for feline coronavirus: A review","volume":"2010","author":"Sharif","year":"2010","journal-title":"Vet. Med. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1177\/1098612X12441875","article-title":"Risk factors for feline infectious peritonitis in Australian cats","volume":"14","author":"Worthing","year":"2012","journal-title":"J. Feline Med. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1177\/1098612X18758592","article-title":"Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests","volume":"20","author":"Tasker","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.tvjl.2013.08.023","article-title":"Diagnostic utility of a direct immunofluorescence test to detect feline coronavirus antigen in macrophages in effusive feline infectious peritonitis","volume":"198","author":"Litster","year":"2013","journal-title":"Vet. J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Sawicki, S., and Sawicki, D. (1998). A new model for coronavirus transcription. Coronaviruses and Arteriviruses, Springer.","DOI":"10.1007\/978-1-4615-5331-1_26"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1099\/vir.0.19129-0","article-title":"Persistence and transmission of natural type I feline coronavirus infection","volume":"84","author":"Addie","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1128\/jcm.33.3.684-689.1995","article-title":"Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR","volume":"33","author":"Herrewegh","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","first-page":"1209","article-title":"Feline coronavirus serotypes 1 and 2: Seroprevalence and association with disease in Switzerland","volume":"12","author":"Kummrow","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"413","DOI":"10.56808\/2985-1130.2419","article-title":"Molecular detection and genotype differentiation of feline coronavirus isolates from clinical specimens in Thailand","volume":"42","author":"Techangamsuwan","year":"2012","journal-title":"Thail. J. Vet. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/tbed.13081","article-title":"Circulation and genetic diversity of Feline coronavirus type I and II from clinically healthy and FIP-suspected cats in China","volume":"66","author":"Li","year":"2018","journal-title":"Transbound Emerg. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1292\/jvms.13-0094","article-title":"Detection of ascitic feline coronavirus RNA from cats with clinically suspected feline infectious peritonitis","volume":"75","author":"Soma","year":"2013","journal-title":"J. Vet. Med. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.vetmic.2003.07.010","article-title":"Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis","volume":"99","author":"Benetka","year":"2004","journal-title":"Vet. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-015-0372-2","article-title":"Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 gene","volume":"11","author":"Tanaka","year":"2015","journal-title":"BMC Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.prevetmed.2015.01.017","article-title":"A retrospective clinical and epidemiological study on feline coronavirus (FCoV) in cats in Istanbul, Turkey","volume":"119","author":"Tekelioglu","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.vetmic.2008.11.010","article-title":"Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: A 5-year study in Taiwan","volume":"136","author":"Lin","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1751-0813.2006.tb13114.x","article-title":"The relationship between the feline coronavirus antibody titre and the age, breed, gender and health status of Australian cats","volume":"84","author":"Bell","year":"2006","journal-title":"Aust. Vet. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.jfms.2009.08.005","article-title":"Prevalence of feline coronavirus in two cat populations in Malaysia","volume":"11","author":"Sharif","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.2460\/javma.2001.218.1111","article-title":"Epidemiology of feline infectious peritonitis among cats examined at veterinary medical teaching hospitals","volume":"218","author":"Rohrbach","year":"2001","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1111\/j.1751-0813.2005.tb13044.x","article-title":"Clinicopathological findings associated with feline infectious peritonitis in Sydney, Australia: 42 cases (1990\u20132002)","volume":"83","author":"Norris","year":"2005","journal-title":"Aust. Vet. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1177\/1098612X15586209","article-title":"Clinical and laboratory features of cats with feline infectious peritonitis\u2014A retrospective study of 231 confirmed cases (2000\u20132010)","volume":"18","author":"Riemer","year":"2015","journal-title":"J. Feline Med. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1292\/jvms.54.557","article-title":"The prevalence of types I and II feline coronavirus infections in cats","volume":"54","author":"Hohdatsu","year":"1992","journal-title":"J. Vet. Med. Sci."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/9\/698\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:06:26Z","timestamp":1760177186000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/9\/9\/698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,25]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["pathogens9090698"],"URL":"https:\/\/doi.org\/10.3390\/pathogens9090698","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,25]]}}}