{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:22:17Z","timestamp":1776424937126,"version":"3.51.2"},"reference-count":46,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AMLR (Romanian Association of Laboratory Medicine)","award":["9103\/23.07.2019"],"award-info":[{"award-number":["9103\/23.07.2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic Escherichia coli, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mure\u0219 County Hospital, between May 2016 and July 2019, 160 diarrheagenic E. coli strains were isolated from children under 2 years old with diarrheic disease. The strains were identified as Shiga toxin-producing E. coli (STEC)\/enteropathogenic Escherichia coli (EPEC) via agglutination with polyvalent sera. STEC strains were serotyped using monovalent sera for serogroup O157. Simplex PCR was performed on the strains to determine the presence of the hlyA gene, and, for the positive ones, the hemolytic activity was tested. Antibiotic susceptibility of the identified diarrheagenic E. coli strains was also investigated. STEC strains were the most frequently identified (49.1%), followed by EPEC (40.2%). The hlyA gene was identified in 12 cases, representing 18.2% of the STEC strains. Even if the extended-spectrum \u03b2-lactamase (ESBL)-producing strains represented only 10%, a relevant percentage of multidrug-resistant (MDR) strains (24%) was identified.<\/jats:p>","DOI":"10.3390\/pathogens9121022","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T11:59:00Z","timestamp":1607083140000},"page":"1022","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hemolysin-Producing Strains among Diarrheagenic Escherichia coli Isolated from Children under 2 Years Old with Diarrheal Disease"],"prefix":"10.3390","volume":"9","author":[{"given":"Anca","family":"Mare","sequence":"first","affiliation":[{"name":"Department of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5281-0601","authenticated-orcid":false,"given":"Adrian","family":"Man","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felicia","family":"Toma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2262-7456","authenticated-orcid":false,"given":"Cristina Nicoleta","family":"Ciurea","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u0103zvan Lucian","family":"Co\u0219eriu","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory, Mure\u0219 County Clinical Hospital, 540233 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camelia","family":"Vintil\u0103","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory, Mure\u0219 County Clinical Hospital, 540233 T\u00e2rgu Mure\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian Cornel","family":"Maier","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University \u201cDunarea de Jos\u201d Gala\u021bi, 800008 Gala\u021bi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1097\/MPG.0000000000000375","article-title":"European society for pediatric gastroenterology, hepatology, and nutrition\/European society for pediatric infectious diseases evidence-based guidelines for the management of acute gastroenteritis in children in Europe","volume":"59","author":"Guarino","year":"2014","journal-title":"J. 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