{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:26:40Z","timestamp":1767155200113,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Initiative for Global Research Network on Infectious Diseases","award":["No Grant Number"],"award-info":[{"award-number":["No Grant Number"]}]},{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","award":["No Grant Number"],"award-info":[{"award-number":["No Grant Number"]}],"id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["No Grant Number"],"award-info":[{"award-number":["No Grant Number"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne mcr genes, is an emerging public health concern. The complete genome sequence (4.55\u2009Mb) of a clinical isolate of Aeromonas veronii biovar veronii obtained from a patient with septicemia was determined using short-read and long-read platforms. This isolate (C198) was found to harbor a novel mcr-3 gene, designated mcr-3.41. Isolate C198 revealed adjacent mcr-3.41 and mcr-3-like genes. It contained one chromosome and two plasmids, both of which encoded a RepB replication protein. Other antimicrobial resistance genes, including blacphA3, blaOXA-12, tetA, rsmA, and adeF, were also present. Isolate C198 was resistant to amoxicillin\u2013clavulanate, ampicillin\u2013sulbactam and tetracycline, and showed intermediate resistance to trimethoprim\u2013sulfamethoxazole. The isolate was susceptible to piperacillin\u2013tazobactam, carbapenem, third-generation cephalosporins, fluoroquinolones, chloramphenicol, and aminoglycosides. Putative virulence genes in the C198 genome encoded type II, III, and VI secretion systems; type IV Aeromonas pili; and type I fimbria, flagella, hemagglutinin, aerolysin, and hemolysins. Multilocus sequence typing revealed a novel sequence type (ST), ST720 for C198. Phylogenetic analysis of the single nucleotide polymorphisms in C198 demonstrated that the strain was closely related to A. veronii 17ISAe. The present study provides insights into the genomic characteristics of human A. veronii isolates.<\/jats:p>","DOI":"10.3390\/pathogens9121031","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T09:17:58Z","timestamp":1607505478000},"page":"1031","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Genomic Analysis of Aeromonas veronii C198, a Novel Mcr-3.41-Harboring Isolate from a Patient with Septicemia in Thailand"],"prefix":"10.3390","volume":"9","author":[{"given":"Rujirat","family":"Hatrongjit","sequence":"first","affiliation":[{"name":"Department of General Sciences, Faculty of Science and Engineering, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-3656","authenticated-orcid":false,"given":"Anusak","family":"Kerdsin","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thidathip","family":"Wongsurawat","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA"},{"name":"Division of Bioinformatics and Data Management for Research, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-401X","authenticated-orcid":false,"given":"Piroon","family":"Jenjaroenpun","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA"},{"name":"Division of Bioinformatics and Data Management for Research, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5010-4682","authenticated-orcid":false,"given":"Peechanika","family":"Chopjitt","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parichart","family":"Boueroy","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukihiro","family":"Akeda","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"},{"name":"Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeyuki","family":"Hamada","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Bravo, A., and Figueras, M.J. 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