{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T14:50:28Z","timestamp":1767451828714,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dun\u0103rea de Jos University of Gala\u021bi"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Reports"],"abstract":"<jats:p>Periodontal diseases, as an important part of oral pathology, present different characteristics when affecting children and adolescents or young adults. Studies have shown that adolescence and childhood are closely related to a high risk of periodontal disease, but the follow-up for periodontal health or damage at this age has been insufficiently appreciated until now. The aim of this study was to identify subgingival microorganisms using a real-time polymerase chain reaction (PCR) in a group of children and adolescents aged 7\u201317 years with and without cardiovascular disease. The group of 62 subjects with gingival inflammation and poor hygiene was divided into two groups according to general condition: 31 subjects with carduivascular disease (group A) and 31 subjects without cardiovascular disease (group C). Subjects were examined in the initial consultation, the state of hygiene and periodontal inflammation was assessed using the plaque index (PI) and gingival index (GI), and samples were taken from the gingival sulcus using sterile paper cones to determine nine subgingival microorganisms. Nine subgingival microorganisms were identified: Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tannerella forsythias (Tf), Prevotella intermedia (Pi), Peptostreptococcus (Micromonas) micros (Pm), Fusobacterium nucleatum (Fn), Eubacterium nodatum (En), and Capnocytophaga gingivalis (Cg). The patients were included in a specialist treatment program which aimed to relieve the inflammatory condition, remove local irritative factors, and train the patients to perform proper oral hygiene at home by using primary and secondary oral hygiene products. Subjects were reevaluated 3 months after treatment, when measurements for the PI and GI and microbiological determinations were repeated. The results showed a predominance of subjects aged 16\u201317 years (12.4%). Among the subjects with marked gingival inflammation, the male gender was predominant (58.06%). The PI values changed considerably after treatment, with lower values in patients presenting a general condition without cardiovascular disease (PI = 8.10%) compared with the patients with cardiovascular disease (PI = 13.77%). After treatment, the GI showed considerable changes in both groups. Red, orange, and purple complex microorganisms were found before treatment and decreased considerably after treatment in both groups. The highest values were found for Treponema denticola (140,000 (1.4 \u00d7 105)) in patients with cardiovascular disease and generalized gingival inflammation. Of the pathogenic microorganisms, the most common was Tannerella forsythia in 52 patients before treatment, and red microorganisms considerably appeared in only 10 patients after treatment. Capnocytophaga gingivalis remained constant both in the diseased state and after treatment and was consistent with periodontal health. Children with cardiovascular diseases had a higher prevalence of gingival manifestations. The composition of the subgingival microbial plaque was directly influenced by the degree of oral hygiene, but the response to specialized treatment was also influenced by the general health status. The results of this study support the conclusion that periodontal pathogens appear and multiply in the absence of proper hygiene in childhood after the eruption of permanent teeth, and their action leads to the initiation of periodontal diseases.<\/jats:p>","DOI":"10.3390\/pediatric16020041","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T11:11:28Z","timestamp":1718709088000},"page":"482-503","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Clinical Study and Microbiological Analysis of Periodontopathogenic Microflora Analyzed among Children and Adolescents with Cardiovascular Diseases Compared to Group with Good General Status"],"prefix":"10.3390","volume":"16","author":[{"given":"Oana","family":"Chipirliu","sequence":"first","affiliation":[{"name":"Research Centre in the Medical-Pharmaceutical Field, Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, 47 Domneasca Str., 800181 Galati, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian Viorel","family":"Cr\u0103ciun","sequence":"additional","affiliation":[{"name":"Research Centre in the Faculty of Automation, Computers, Electrical and Electronics Engineering, Dunarea de Jos University of Galati, 111 Domneasca Str., 800181 Galati, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madalina Nicoleta","family":"Matei","sequence":"additional","affiliation":[{"name":"Research Centre in the Medical-Pharmaceutical Field, Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, 47 Domneasca Str., 800181 Galati, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"ref_1","first-page":"441","article-title":"Microbiology of dental plaque biofilms and their role in oral health and caries","volume":"54","author":"Marsh","year":"2010","journal-title":"Dent. Clin."},{"unstructured":"Newman, M.G., Takei, H., Klokkevold, P.R., and Carranza, F.A. (2018). Newman and Carranza\u2019s Clinical Periodontology: Newman and Carranza\u2019s Clinical Periodontology E-Book, Elsevier Health Sciences.","key":"ref_2"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1016\/j.jmb.2019.05.016","article-title":"The structure of dental plaque microbial communities in the transition from health to dental caries and periodontal disease","volume":"431","author":"Valm","year":"2019","journal-title":"J. Mol. Biol."},{"doi-asserted-by":"crossref","unstructured":"Inquimbert, C., Bourgeois, D., Bravo, M., Viennot, S., Tramini, P., Llodra, J.C., Molinari, N., Dussart, C., Giraudeau, N., and Carrouel, F. (2019). The oral bacterial microbiome of interdental surfaces in adolescents according to carious risk. Microorganisms, 7.","key":"ref_4","DOI":"10.3390\/microorganisms7090319"},{"unstructured":"Roman, A. Parodontologie vol. 1, No\u021biuni de baz\u0103, Ed Med Univ Iuliu Hatieganuu 2017.","key":"ref_5"},{"unstructured":"Olani\u00e9, F. (2024, April 15). Les Tests Biologiques en Parodontologie. Doctoral Dissertation. Available online: https:\/\/paroconseil.ca\/wp-content\/uploads\/2020\/11\/Les-tests-biologiques-en-parodontologie.pdf.","key":"ref_6"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1600-051X.1998.tb02419.x","article-title":"Microbial complexes in subgingival plaque","volume":"25","author":"Socransky","year":"1988","journal-title":"J. Clin. Periodontol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3897\/folmed.62.e47567","article-title":"Depth of Gingival Sulcus in Healthy Children with Erupting Permanent Teeth","volume":"62","author":"Mitova","year":"2020","journal-title":"Folia Medica"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"128","DOI":"10.37897\/RJS.2021.2.11","article-title":"Detection of bacterial species associated with periodontal disease in a group of patients","volume":"67","author":"Ghergic","year":"2021","journal-title":"Rom. J. Stomatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1034\/j.1600-0757.2002.280102.x","article-title":"Dental biofilms: Difficult therapeutic targets","volume":"28","author":"Socransky","year":"2002","journal-title":"Periodontol. 2000"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"26","DOI":"10.4103\/2277-4696.159114","article-title":"Gingival and periodontal diseases in children and adolescents","volume":"1","author":"Chauhan","year":"2012","journal-title":"J. Dent. Allied Sci."},{"key":"ref_12","first-page":"1","article-title":"Periodontal diseases in children and adolescents affected by systemic disorders\u2014A literature review","volume":"4","author":"Vagdouti","year":"2018","journal-title":"Int. J. Oral. Dent. Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1034\/j.1600-0757.2001.2260105.x","article-title":"Influences of systemic diseases on periodontitis in children and adolescents","volume":"26","author":"Meyle","year":"2001","journal-title":"Periodontol. 2000"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/prd.12015","article-title":"Aggressive forms of periodontitis secondary to systemic disorders","volume":"65","author":"Khocht","year":"2014","journal-title":"Periodontol. 2000"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S44","DOI":"10.1111\/jcpe.12939","article-title":"Plaque-induced gingivitis: Case definition and diagnostic considerations","volume":"45","author":"Trombelli","year":"2018","journal-title":"J. Clin. Periodontol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1308\/135576110790307690","article-title":"Dental health and management for children with congenital heart disease","volume":"os17","author":"FitzGerald","year":"2010","journal-title":"Prim. Dent. Care"},{"key":"ref_17","first-page":"456","article-title":"Gingival evaluation of the pediatric cardiac patient","volume":"35","author":"Nosrati","year":"2013","journal-title":"Pediatr. Dent."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2334\/josnusd.16-0856","article-title":"The oral microbiome and human health","volume":"59","author":"Takeshita","year":"2017","journal-title":"J. Oral Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S51","DOI":"10.1902\/jop.2013.134006","article-title":"Inflammatory mechanisms linking periodontal diseases to cardiovascular diseases","volume":"84","author":"Schenkein","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"328","DOI":"10.12968\/denu.2014.41.4.328","article-title":"Simplified basic periodontal examination (BPE) in children and adolescents: A guide for general dental practitioners","volume":"41","author":"Cole","year":"2014","journal-title":"Dent. Update"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"541","DOI":"10.12968\/denu.2012.39.8.541","article-title":"Periodontal diseases in children and adolescents: A clinician\u2019s perspective part 1","volume":"39","author":"Masamatti","year":"2012","journal-title":"Dent. Update"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"639","DOI":"10.12968\/denu.2012.39.9.639","article-title":"Periodontal diseases in children and adolescents: A clinician\u2019s perspective part 2","volume":"39","author":"Kumar","year":"2012","journal-title":"Dent. Update"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1080\/13102818.2019.1604156","article-title":"Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis","volume":"33","author":"Mitova","year":"2019","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s00784-014-1316-0","article-title":"Real-time PCR quantification of six periodontal pathogens in saliva samples from healthy young adults","volume":"19","author":"Zhou","year":"2015","journal-title":"Clin. Oral Investig."},{"unstructured":"Asocia\u0163ia Societatea Rom\u00e2n\u0103 (2013). Ghid de Practic\u0103 \u00een Preven\u0163ia Oro-Dentar\u0103, Asocia\u0163ia Societatea Rom\u00e2n\u0103 de Ergonomie Dentar\u0103.","key":"ref_25"},{"doi-asserted-by":"crossref","unstructured":"Shimpi, N., Dart, R., Umukoro, P., and Acharya, A. (2019). Interdisciplinary care model: Cardiovascular diseases and oral health. Integration of Medical and Dental Care and Patient Data, Springer.","key":"ref_26","DOI":"10.1007\/978-3-319-98298-4_6"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e86","DOI":"10.4081\/cp.2012.e86","article-title":"Oral hygiene status of individuals with cardiovascular diseases and associated risk factors","volume":"2","author":"Shetty","year":"2012","journal-title":"Clin. Pract."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1097\/HJH.0000000000003022","article-title":"Association between oral health and cardiovascular outcomes in patients with hypertension: A nationwide cohort study","volume":"40","author":"Kim","year":"2022","journal-title":"J. Hypertens."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.micinf.2005.09.004","article-title":"Detection of bacterial DNA in atheromatous plaques by quantitative PCR","volume":"8","author":"Kozarov","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1128\/JCM.00569-09","article-title":"Porphyromonas gingivalis strain diversity","volume":"47","author":"Igboin","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1902\/jop.2004.75.8.1077","article-title":"Occurrence of Porphyromonas gingivalis and Tannerella forsythensis in periodontally diseased and healthy subjects","volume":"75","author":"Yang","year":"2004","journal-title":"J. Periodontol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.mib.2014.11.013","article-title":"Fusobacterium nucleatum: A commensal-turned pathogen","volume":"23","author":"Han","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.tcm.2023.03.003","article-title":"Oral health as a modifiable risk factor for cardiovascular diseases","volume":"34","author":"Altamura","year":"2023","journal-title":"Trends Cardiovasc. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1128\/jb.129.1.9-14.1977","article-title":"Chemical structure of the lipid A component of lipopolysaccharides from Fusobacterium nucleatum","volume":"129","author":"Hase","year":"1977","journal-title":"J. Bacteriol."},{"key":"ref_35","first-page":"371","article-title":"O-antigenic cross-reactivity in Fusobacterium nucleatum","volume":"87","author":"Hofstad","year":"1979","journal-title":"Acta Pathol. Microbiol. Scand. Sect. B Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.0902-0055.2004.00132.x","article-title":"Identification of a Fusobacterium nucleatum 65 kDa serine protease","volume":"19","author":"Bachrach","year":"2004","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1016\/j.archoralbio.2012.02.003","article-title":"Subgingival microbial profiles of generalized aggressive and chronic periodontal diseases","volume":"57","author":"Heller","year":"2012","journal-title":"Arch. Oral Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/jre.13173","article-title":"New putative periodontopathogens and periodontal health-associated species: A systematic review and meta-analysis","volume":"58","author":"Antezack","year":"2023","journal-title":"J. Periodontal Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"718441","DOI":"10.3389\/fdmed.2021.718441","article-title":"Exploring the interplay between oral diseases, microbiome, and chronic diseases driven by metabolic dysfunction in childhood","volume":"2","author":"Sabella","year":"2021","journal-title":"Front. Dent. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1007\/s13238-018-0548-1","article-title":"Oral microbiomes: More and more importance in oral cavity and whole body","volume":"9","author":"Gao","year":"2018","journal-title":"Protein Cell"}],"container-title":["Pediatric Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2036-7503\/16\/2\/41\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:00:30Z","timestamp":1760108430000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2036-7503\/16\/2\/41"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,18]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["pediatric16020041"],"URL":"https:\/\/doi.org\/10.3390\/pediatric16020041","relation":{},"ISSN":["2036-7503"],"issn-type":[{"type":"electronic","value":"2036-7503"}],"subject":[],"published":{"date-parts":[[2024,6,18]]}}}