{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:44:45Z","timestamp":1762267485656,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Reports"],"abstract":"<jats:p>Background\/Objectives: Autism Spectrum Disorder (ASD) is a neurodevelopmental condition characterized by impairments in social interaction and communication, as well as by repetitive behaviors, with a rising global prevalence. Concurrently, the use of Assisted Reproductive Technologies (ART) has increased among couples experiencing infertility. This study aimed to compare the frequency of ART-conceived children between those diagnosed with ASD and those with other neurodevelopmental disorders (nASD), and to examine differences in prenatal, perinatal and medical histories of ART- and spontaneously (non-ART)-conceived children within an ASD group. Methods: We retrospectively analyzed data from 507 children with a neurodevelopmental disorders (NDDs) diagnosis, classified into ASD (n = 234) and nASD (n = 273) groups. Subsequent analyses focused on the ASD group, further divided into an ART and non-ART group according to the conception mode. Results: ART-conceived children were more frequent in the ASD group than in the nASD group. Moreover, within ASD, ART was significantly associated with potential risk factors such as twin pregnancy, cesarean delivery, low birth weight and parental age. Logistic Binary Regression confirmed these results, suggesting that ART co-occurs with a cluster of perinatal and familial risk factors. Conclusions: Our results indicate that ART is not an independent causal exposure; however, given the retrospective design and the absence of a general population control group, causal inference cannot be drawn. The observed association with ASD appears to be mediated by perinatal and parental variables. These findings underscore the importance of improving obstetric management and care, and ensuring early developmental monitoring for ART-conceived children.<\/jats:p>","DOI":"10.3390\/pediatric17060118","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:02:09Z","timestamp":1762264929000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Association Between Medically Assisted Reproduction and Autism Spectrum Disorder: Clinical Correlations from a Retrospective Cohort"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9240-3418","authenticated-orcid":false,"given":"Federica","family":"Gigliotti","sequence":"first","affiliation":[{"name":"Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7381-9252","authenticated-orcid":false,"given":"Maria Eugenia","family":"Martelli","sequence":"additional","affiliation":[{"name":"Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Foglietta","sequence":"additional","affiliation":[{"name":"Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessia","family":"Balestrini","sequence":"additional","affiliation":[{"name":"Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0589-7614","authenticated-orcid":false,"given":"Carla","family":"Sogos","sequence":"additional","affiliation":[{"name":"Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S2215-0366(16)30376-5","article-title":"Neurodevelopmental disorders","volume":"4","author":"Thapar","year":"2017","journal-title":"Lancet Psychiatry"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1017\/S003329171400172X","article-title":"The epidemiology and global burden of autism spectrum disorders","volume":"45","author":"Baxter","year":"2015","journal-title":"Psychol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss7011a1","article-title":"Prevalence and Characteristics of Autism Spectrum Disorder Among Children Aged 8 Years\u2014Autism and Developmental Disabilities Monitoring Network, 11 Sites, United States, 2018","volume":"70","author":"Maenner","year":"2021","journal-title":"Surveill. Summ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1001\/jamapediatrics.2014.210","article-title":"Costs of autism spectrum disorders in the United Kingdom and the United States","volume":"168","author":"Buescher","year":"2014","journal-title":"JAMA Pediatr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13229-017-0121-4","article-title":"Environmental risk factors for autism: An evidence-based review of systematic reviews and meta-analyses","volume":"8","author":"Modabbernia","year":"2017","journal-title":"Mol. Autism"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/humupd\/dmv016","article-title":"Infertility around the globe: New thinking on gender, reproductive technologies and global movements in the 21st century","volume":"21","author":"Inhorn","year":"2015","journal-title":"Hum. Reprod. Updat."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1093\/humrep\/deab198","article-title":"International Committee for Monitoring Assisted Reproductive Technologies world report: Assisted reproductive technology, 2014","volume":"36","author":"Chambers","year":"2021","journal-title":"Hum. Reprod."},{"key":"ref_8","unstructured":"European IVF-Monitoring Consortium (EIM) for the European Society of Human Reproduction and Embryology (ESHRE), Wyns, C., De Geyter, C., Calhaz-Jorge, C., Kupka, M.S., Motrenko, T., Smeenk, J., Bergh, C., Tandler-Schneider, A., and Rugescu, I.A. (2021). ART in Europe, 2017: Results generated from European registries by ESHRE. Hum. Reprod. Open, 2021, hoab026."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/humupd\/dmz001","article-title":"The health of children conceived by ART: \u2018the chicken or the egg?\u2019","volume":"25","author":"Berntsen","year":"2019","journal-title":"Hum. Reprod. Updat."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1056\/NEJMoa1008095","article-title":"Reproductive Technologies and the Risk of Birth Defects","volume":"366","author":"Davies","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.fertnstert.2022.01.010","article-title":"The neurodevelopmental morbidity of children born after assisted reproductive technology: A Nordic register study from the Committee of Nordic Assisted Reproductive Technology and Safety group","volume":"117","author":"Rissanen","year":"2022","journal-title":"Fertil. Steril."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1136\/archdischild-2015-309581","article-title":"Early and long-term outcome of infants born extremely preterm","volume":"102","author":"Johnson","year":"2017","journal-title":"Arch. Dis. Child."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liu, L., Gao, J., He, X., Cai, Y., Wang, L., and Fan, X. (2017). Association between assisted reproductive technology and the risk of autism spectrum disorders in the offspring: A meta-analysis. Sci. Rep., 7.","DOI":"10.1038\/srep46207"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s11689-020-09347-w","article-title":"Risk of neurodevelopmental disorders in children born from different ART treatments: A systematic review and meta-analysis","volume":"12","author":"Djuwantono","year":"2020","journal-title":"J. Neurodev. Disord."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.1007\/s00431-021-04187-9","article-title":"Association of assisted reproductive technology with autism spectrum disorder in the offspring: An updated systematic review and meta-analysis","volume":"180","author":"Andreadou","year":"2021","journal-title":"Eur. J. Pediatr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"963","DOI":"10.2105\/AJPH.2014.302383","article-title":"Association Between Assisted Repro-ductive Technology Conception and Autism in California, 1997\u20132007","volume":"105","author":"Fountain","year":"2015","journal-title":"Am. J. Public Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1093\/aje\/kwae049","article-title":"The role of multiple birth and birth complications in the association between assisted reproductive technology conception and autism diagnosis","volume":"193","author":"Winter","year":"2024","journal-title":"Am. J. Epidemiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1093\/ije\/dyt262","article-title":"Parental age and the risk of autism spectrum disorders: Findings from a Swedish population-based cohort","volume":"43","author":"Idring","year":"2014","journal-title":"Int. J. Epidemiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1001\/jamapsychiatry.2013.4525","article-title":"Paternal Age at Childbearing and Offspring Psychiatric and Academic Morbidity","volume":"71","author":"Rickert","year":"2014","journal-title":"JAMA Psychiatry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1038\/ng.3597","article-title":"Parent-of-origin-specific signatures of de novo mutations","volume":"48","author":"Goldmann","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"160271","DOI":"10.1038\/npjgenmed.2016.27","article-title":"Genome-wide characteristics of de novo mutations in autism","volume":"1","author":"Yuen","year":"2016","journal-title":"NPJ Genom. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/s41588-019-0344-8","article-title":"Identification of common genetic risk variants for autism spectrum disorder","volume":"51","author":"Grove","year":"2019","journal-title":"Nat. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1007\/s10826-018-1026-3","article-title":"Broader autism phenotype in parents of children with autism: A systematic review of percentage estimates","volume":"27","author":"Rubenstein","year":"2018","journal-title":"J. Child. Fam. Stud."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1093\/hmg\/ddp319","article-title":"DNA methylation and gene expression differences in children conceived in vitro or in vivo","volume":"18","author":"Katari","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3922","DOI":"10.1038\/s41467-019-11929-9","article-title":"Assisted reproductive technologies are associated with limited epigenetic variation at birth that largely resolves by adulthood","volume":"10","author":"Novakovic","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"ESHRE Guideline Group on the Number of Embryos to Transfer, Alteri, A., Arroyo, G., Baccino, G., Craciunas, L., De Geyter, C., Ebner, T., Koleva, M., Kordic, K., and Mcheik, S. (2024). ESHRE guideline: Number of embryos to transfer during IVF\/ICSI. Hum. Reprod., 39, 647\u2013657.","DOI":"10.1093\/humrep\/deae010"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/j.1365-3016.2009.01062.x","article-title":"Self-selection and bias in a large prospective pregnancy cohort in Norway","volume":"23","author":"Nilsen","year":"2009","journal-title":"Paediatr. Perinat. Epidemiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1093\/ije\/dyw029","article-title":"Cohort profile update: The Norwegian Mother and Child Cohort Study (MoBa)","volume":"45","author":"Magnus","year":"2016","journal-title":"Int. J. Epidemiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1111\/ppe.12821","article-title":"Lost to follow-up in the Norwegian mother, father and child cohort study","volume":"36","author":"Vejrup","year":"2021","journal-title":"Paediatr. Perinat. Epidemiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1053\/j.semperi.2013.06.004","article-title":"Benefits and risks of ultrasound in pregnancy","volume":"37","author":"Abramowicz","year":"2013","journal-title":"Semin. Perinatol."}],"container-title":["Pediatric Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2036-7503\/17\/6\/118\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:38:40Z","timestamp":1762267120000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2036-7503\/17\/6\/118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"references-count":30,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["pediatric17060118"],"URL":"https:\/\/doi.org\/10.3390\/pediatric17060118","relation":{},"ISSN":["2036-7503"],"issn-type":[{"value":"2036-7503","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]}}}