{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:15:54Z","timestamp":1783415754312,"version":"3.54.6"},"reference-count":20,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T00:00:00Z","timestamp":1484784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA 3309\/1-3"],"award-info":[{"award-number":["HA 3309\/1-3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Interdisciplinary Centre for Clinical Research at the University Hospital of the University of Erlangen-Nuremberg","award":["IZKF E2, E17"],"award-info":[{"award-number":["IZKF E2, E17"]}]},{"name":"Johannes und Frieda Marohn-Stiftung"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The tetrameric protein kinase CK2 was identified playing a role at neuromuscular junctions by studying CK2\u03b2-deficient muscle fibers in mice, and in cultured immortalized C2C12 muscle cells after individual knockdown of CK2\u03b1 and CK2\u03b2 subunits. In muscle cells, CK2 activity appeared to be at least required for regular aggregation of nicotinic acetylcholine receptors, which serves as a hallmark for the presence of a postsynaptic apparatus. Here, we set out to determine whether any other feature accompanies CK2\u03b2-deficient muscle fibers. Hind limb muscles gastrocnemius, plantaris, and soleus of adult wildtype and CK2\u03b2-deficient mice were dissected, cross-sectioned, and stained histochemically by Gomori trichrome and for nicotinamide adenine dinucleotide (NADH) dehydrogenase and succinate dehydrogenase (SDH) enzymatic activities. A reduction of oxidative enzymatic activity was determined for CK2\u03b2-deficient muscle fibers in comparison with wildtype controls. Importantly, the CK2\u03b2-deficient fibers, muscle fibers that typically exhibit high NADH dehydrogenase and SDH activities, like slow-type fibers, showed a marked reduction in these activities. Altogether, our data indicate additional impairments in the absence of CK2\u03b2 in skeletal muscle fibers, pointing to an eventual mitochondrial myopathy.<\/jats:p>","DOI":"10.3390\/ph10010013","type":"journal-article","created":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T10:55:40Z","timestamp":1484823340000},"page":"13","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ablation of Protein Kinase CK2\u03b2 in Skeletal Muscle Fibers Interferes with Their Oxidative Capacity"],"prefix":"10.3390","volume":"10","author":[{"given":"Nane","family":"Eiber","sequence":"first","affiliation":[{"name":"Institute of Biochemistry, Friedrich-Alexander University of Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Simeone","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Friedrich-Alexander University of Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6564-5649","authenticated-orcid":false,"given":"Said","family":"Hashemolhosseini","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Friedrich-Alexander University of Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1139\/o04-116","article-title":"Order or chaos? An evaluation of the regulation of protein kinase ck2","volume":"82","author":"Olsten","year":"2004","journal-title":"Biochem. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1096\/fj.02-0473rev","article-title":"One-thousand-and-one substrates of protein kinase ck2?","volume":"17","author":"Meggio","year":"2003","journal-title":"FASEB J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/mrd.10346","article-title":"Spermatogenesis of mice lacking ck2\u03b1\u2032: Failure of germ cell survival and characteristic modifications of the spermatid nucleus","volume":"66","author":"Escalier","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/12729","article-title":"Globozoospermia in mice lacking the casein kinase II \u03b1\u2032 catalytic subunit","volume":"23","author":"Xu","year":"1999","journal-title":"Nat. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1128\/MCB.23.3.908-915.2003","article-title":"Disruption of the regulatory beta subunit of protein kinase ck2 in mice leads to a cell-autonomous defect and early embryonic lethality","volume":"23","author":"Buchou","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"24018","DOI":"10.1074\/jbc.M212260200","article-title":"Ck2 phosphorylation of the armadillo repeat region of beta-catenin potentiates wnt signaling","volume":"278","author":"Song","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1093\/brain\/awf095","article-title":"A newly identified chromosomal microdeletion and an n-box mutation of the achr epsilon gene cause a congenital myasthenic syndrome","volume":"125","author":"Abicht","year":"2002","journal-title":"Brain"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/S0896-6273(02)00783-3","article-title":"Regulation of achr clustering by dishevelled interacting with musk and pak1","volume":"35","author":"Luo","year":"2002","journal-title":"Neuron"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1038\/nn1128","article-title":"Regulation of acetylcholine receptor clustering by the tumor suppressor APC","volume":"6","author":"Wang","year":"2003","journal-title":"Nat. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.tins.2004.06.015","article-title":"Wnt signaling in axon guidance","volume":"27","author":"Zou","year":"2004","journal-title":"Trends Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1242\/dev.139907","article-title":"Wnt\/beta-catenin signaling via axin2 is required for myogenesis and, together with yap\/taz and tead1, active in iia\/iix muscle fibers","volume":"143","author":"Huraskin","year":"2016","journal-title":"Development"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0014-5793(82)81332-X","article-title":"Multisite phosphorylation of glycogen synthase from rabbit skeletal muscle. Phosphorylation of site 5 by glycogen synthase kinase-5 (casein kinase-ii) is a prerequisite for phosphorylation of sites 3 by glycogen synthase kinase-3","volume":"150","author":"Picton","year":"1982","journal-title":"FEBS Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1101\/gad.375206","article-title":"Casein kinase 2-dependent serine phosphorylation of musk regulates acetylcholine receptor aggregation at the neuromuscular junction","volume":"20","author":"Cheusova","year":"2006","journal-title":"Genes Dev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"22370","DOI":"10.1074\/jbc.M115.647610","article-title":"Protein kinase ck2 interacts at the neuromuscular synapse with rapsyn, rac1, 14-3-3gamma, and dok-7 proteins and phosphorylates the latter two","volume":"290","author":"Herrmann","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s11010-008-9819-0","article-title":"Examination of transcript amounts and activity of protein kinase ck2 in muscle lysates of different types of human muscle pathologies","volume":"316","author":"Heuss","year":"2008","journal-title":"Mol. Cell Biochem."},{"key":"ref_16","unstructured":"Thompson, S.W. (1966). Selected Histochemical and Histopathological Methods, C.C. Thomas."},{"key":"ref_17","unstructured":"Sheehan, D.C., and Hrapchak, B.B. (1987). Theory and Practice of Histotechnology, Battelle Press. [2nd ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/jnc.13710","article-title":"LAP proteins are localized at the post-synaptic membrane of neuromuscular junctions and appear to modulate synaptic morphology and transmission","volume":"139","author":"Kravic","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6620","DOI":"10.1523\/JNEUROSCI.5778-09.2010","article-title":"Identification of erbin interlinking musk and erbb2 and its impact on acetylcholine receptor aggregation at the neuromuscular junction","volume":"30","author":"Simeone","year":"2010","journal-title":"J. Neurosci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1002\/mrd.22489","article-title":"The imagej ecosystem: An open platform for biomedical image analysis","volume":"82","author":"Schindelin","year":"2015","journal-title":"Mol. Reprod. Dev."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/10\/1\/13\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:26:33Z","timestamp":1760207193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/10\/1\/13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,19]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,3]]}},"alternative-id":["ph10010013"],"URL":"https:\/\/doi.org\/10.3390\/ph10010013","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,19]]}}}