{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:00:58Z","timestamp":1787029258759,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T00:00:00Z","timestamp":1604620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006261","name":"Taif University","doi-asserted-by":"publisher","award":["TURSP-2020\/39"],"award-info":[{"award-number":["TURSP-2020\/39"]}],"id":[{"id":"10.13039\/501100006261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Methicillin-resistant Staphylococcus aureus is a major human pathogen that poses a high risk to patients due to the development of biofilm. Biofilms, are complex biological systems difficult to treat by conventional antibiotic therapy, which contributes to &gt;80% of humans infections. In this report, we examined the antibacterial activity of Origanum majorana, Rosmarinus officinalis, and Thymus zygis medicinal plant essential oils against MRSA clinical isolates using disc diffusion and MIC methods. Moreover, biofilm inhibition and eradication activities of oils were evaluated by crystal violet. Gas chromatography\u2013mass spectrometry analysis revealed variations between oils in terms of component numbers in addition to their percentages. Antibacterial activity testing showed a strong effect of these oils against MRSA isolates, and T. zygis had the highest activity succeeded by O. majorana and R. officinalis. Investigated oils demonstrated high biofilm inhibition and eradication actions, with the percentage of inhibition ranging from 10.20 to 95.91%, and the percentage of eradication ranging from 12.65 to 98.01%. O. majorana oil had the highest biofilm inhibition and eradication activities. Accordingly, oils revealed powerful antibacterial and antibiofilm activities against MRSA isolates and could be a good alternative for antibiotics substitution.<\/jats:p>","DOI":"10.3390\/ph13110369","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T09:09:30Z","timestamp":1604653770000},"page":"369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Biofilm Inhibition and Eradication Properties of Medicinal Plant Essential Oils against Methicillin-Resistant Staphylococcus aureus Clinical Isolates"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5435-9268","authenticated-orcid":false,"given":"Fethi","family":"Ben Abdallah","sequence":"first","affiliation":[{"name":"Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"},{"name":"Unit\u00e9 de Recherche, Virologie &amp; Strat\u00e9gies Antivirales, UR17ES30, Institut Sup\u00e9rieur de Biotechnologie, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rihab","family":"Lagha","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"},{"name":"Unit\u00e9 de Recherche, Virologie &amp; Strat\u00e9gies Antivirales, UR17ES30, Institut Sup\u00e9rieur de Biotechnologie, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8297-935X","authenticated-orcid":false,"given":"Ahmed","family":"Gaber","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"},{"name":"Department of Genetics, Faculty of Agriculture, Cairo University, Giza 12613, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S350","DOI":"10.1086\/533591","article-title":"Pathogenesis of methicillin-resistant Staphylococcus aureus infection","volume":"46","author":"Gordon","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jhin.2012.12.013","article-title":"Tirty-day mortality in UK patients with community-onset and hospital-acquired methicillin susceptible Staphylococcus aureus bacteraemia","volume":"84","author":"Melzer","year":"2013","journal-title":"J. Hosp. 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