{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:08:01Z","timestamp":1781608081109,"version":"3.54.5"},"reference-count":68,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T00:00:00Z","timestamp":1608940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Structural and Investment Funds Operational Program Research, Development and Education","award":["CZ.02.2.69\/0.0\/0.0\/16_027\/0008351"],"award-info":[{"award-number":["CZ.02.2.69\/0.0\/0.0\/16_027\/0008351"]}]},{"DOI":"10.13039\/100014809","name":"Technology Agency of the Czech Republic","doi-asserted-by":"publisher","award":["TJ02000372"],"award-info":[{"award-number":["TJ02000372"]}],"id":[{"id":"10.13039\/100014809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Selaginella P. Beauv. is a group of vascular plants in the family Selaginellaceae Willk., found worldwide and numbering more than 700 species, with some used as foods and medicines. The aim of this paper was to compare methanolic (MeOH) and dichloromethane (DCM) extracts of eight Selaginella species on the basis of their composition and biological activities. Six of these Selaginella species are underinvestigated. Using ultra-high performance liquid chromatography\u2013high-resolution mass spectrometry (UHPLC\u2013HRMS) analysis, we identified a total of 193 compounds among the tested Selaginella species, with flavonoids predominating. MeOH extracts recovered more constituents that were detected, including selaginellins, the occurrence of which is only typical for this plant genus. Of all the tested species, Selaginellaapoda contained the highest number of identified selaginellins. The majority of the compounds were identified in S. apoda, the fewest compounds in Selaginellacupressina. All the tested species demonstrated antioxidant activity using oxygen radical absorption capacity (ORAC) assay, which showed that MeOH extracts had higher antioxidant capacity, with the half maximal effective concentration (EC50) ranging from 12 \u00b1 1 (Selaginellamyosuroides) to 124 \u00b1 2 (Selaginellacupressina) mg\/L. The antioxidant capacity was presumed to be correlated with the content of flavonoids, (neo)lignans, and selaginellins. Inhibition of acetylcholinesterase (AChE) was mostly discerned in DCM extracts and was only exhibited in S. myosuroides, S. cupressina, Selaginellabiformis, and S. apoda extracts with the half maximal inhibitory concentration (IC50) in the range of 19 \u00b1 3 to 62 \u00b1 1 mg\/L. Substantial cytotoxicity against cancer cell lines was demonstrated by the MeOH extract of S. apoda, where the ratio of the IC50 HEK (human embryonic kidney) to IC50 HepG2 (hepatocellular carcinoma) was 7.9 \u00b1 0.2. MeOH extracts inhibited the production of nitrate oxide and cytokines in a dose-dependent manner. Notably, S. biformis halved the production of NO, tumor necrosis factor (TNF)-\u03b1, and interleukin (IL)-6 at the following concentrations: 105 \u00b1 9, 11 \u00b1 1, and 10 \u00b1 1 mg\/L, respectively. Our data confirmed that extracts from Selaginella species exhibited cytotoxicity against cancer cell lines and AChE inhibition. The activity observed in S. apoda was the most promising and is worth further exploration.<\/jats:p>","DOI":"10.3390\/ph14010016","type":"journal-article","created":{"date-parts":[[2020,12,27]],"date-time":"2020-12-27T20:04:58Z","timestamp":1609099498000},"page":"16","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species"],"prefix":"10.3390","volume":"14","author":[{"given":"B\u00e1ra","family":"K\u0159\u00ed\u017ekovsk\u00e1","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7187-937X","authenticated-orcid":false,"given":"Rohitesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7896-7057","authenticated-orcid":false,"given":"Kate\u0159ina","family":"\u0158eho\u0159ov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"S\u00fdkora","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Dobiasov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisa","family":"Ku\u010derov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4413-5460","authenticated-orcid":false,"given":"Maria Carmen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6940-8627","authenticated-orcid":false,"given":"Virgilio","family":"Linis","sequence":"additional","affiliation":[{"name":"Department of Biology, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8224-9034","authenticated-orcid":false,"given":"Glenn","family":"Oyong","sequence":"additional","affiliation":[{"name":"Molecular Science Unit Laboratory, Center for Natural Sciences and Environmental Research, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-4366","authenticated-orcid":false,"given":"Tom\u00e1\u0161","family":"Ruml","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Lipov","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0857-153X","authenticated-orcid":false,"given":"Jitka","family":"Viktorov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 5, 166 28 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2013.00119","article-title":"Chemodiversity in selaginella: A reference system for parallel and convergent metabolic evolution in terrestrial plants","volume":"4","author":"Weng","year":"2013","journal-title":"Front. 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