{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T10:59:52Z","timestamp":1792493992498,"version":"4.5.0"},"reference-count":43,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T00:00:00Z","timestamp":1614816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["CTQ2017-88179-P; SAF2017-88107-R; MDM2017-0767; AEI\/FEDER UE"],"award-info":[{"award-number":["CTQ2017-88179-P; SAF2017-88107-R; MDM2017-0767; AEI\/FEDER UE"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","award":["2017SGR1074; 2017SGR1746"],"award-info":[{"award-number":["2017SGR1074; 2017SGR1746"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["DIN2019-010420"],"award-info":[{"award-number":["DIN2019-010420"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The interaction between drugs and transport proteins, such as albumins, is a key factor in drug bioavailability. One of the techniques commonly used for the evaluation of the drug-protein complex formation is fluorescence. This work studies the interaction of human serum albumin (HSA) with four non-steroidal anti-inflammatory drugs (NSAIDs)\u2014ibuprofen, flurbiprofen, naproxen, and diflunisal\u2014by monitoring the fluorescence quenching when the drug-albumin complex is formed. Two approaches\u2014the double logarithm Stern-Volmer equation and the STAR program\u2014are used to evaluate the binding parameters. The results are analyzed considering the binding properties, determined by using other complementary techniques and the available structural information of albumin complexes with NSAID-related compounds. Finally, this combined analysis has been synergistically used to interpret the binding of flurbiprofen to HSA.<\/jats:p>","DOI":"10.3390\/ph14030214","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T00:39:07Z","timestamp":1614904747000},"page":"214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8976-467X","authenticated-orcid":false,"given":"Susana","family":"Am\u00e9zqueta","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Mart\u00ed i Franqu\u00e8s 1-11, 08028 Barcelona, Spain"},{"name":"Institute of Biomedicine (IBUB), University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-399X","authenticated-orcid":false,"given":"Jos\u00e9 Lu\u00eds","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Mart\u00ed i Franqu\u00e8s 1-11, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Bolioli","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Mart\u00ed i Franqu\u00e8s 1-11, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0751-2247","authenticated-orcid":false,"given":"Llu\u00eds","family":"Campos-Vicens","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Food Science and Gastronomy, Faculty of Pharmacy and Food Science, University of Barcelona, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Pharmacelera, Torre R, 4a planta, Despatx A05, Parc Cient\u00edfic de Barcelona, Baldiri Reixac 8, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-3567","authenticated-orcid":false,"given":"Francisco Javier","family":"Luque","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine (IBUB), University of Barcelona, 08028 Barcelona, Spain"},{"name":"Department of Nutrition, Food Science and Gastronomy, Faculty of Pharmacy and Food Science, University of Barcelona, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut of Theoretical and Computational Chemistry (IQTCUB), University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7811-986X","authenticated-orcid":false,"given":"Clara","family":"R\u00e0fols","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Mart\u00ed i Franqu\u00e8s 1-11, 08028 Barcelona, Spain"},{"name":"Institute of Biomedicine (IBUB), University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,4]]},"reference":[{"key":"ref_1","unstructured":"FDA (2021, January 15). 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