{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T01:34:51Z","timestamp":1772760891158,"version":"3.50.1"},"reference-count":74,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,7]],"date-time":"2021-03-07T00:00:00Z","timestamp":1615075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010897","name":"Newton Fund","doi-asserted-by":"publisher","award":["261876735"],"award-info":[{"award-number":["261876735"]}],"id":[{"id":"10.13039\/100010897","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High Value Chemicals from Plants (BBSRC)","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"name":"Higher Committee for Education Development in Iraq (HCED) award","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/P011241\/1"],"award-info":[{"award-number":["MR\/P011241\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000313","name":"British Society for Antimicrobial Chemotherapy","doi-asserted-by":"publisher","award":["BSAC-2018-0004"],"award-info":[{"award-number":["BSAC-2018-0004"]}],"id":[{"id":"10.13039\/501100000313","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The use of plant-derived natural products for the treatment of tropical parasitic diseases often has ethnopharmacological origins. As such, plants grown in temperate regions remain largely untested for novel anti-parasitic activities. We describe here a screen of the PhytoQuest Phytopure library, a novel source comprising over 600 purified compounds from temperate zone plants, against in vitro culture systems for Plasmodium falciparum, Leishmania mexicana, Trypanosoma evansi and T. brucei. Initial screen revealed 6, 65, 15 and 18 compounds, respectively, that decreased each parasite\u2019s growth by at least 50% at 1\u20132 \u00b5M concentration. These initial hits were validated in concentration-response assays against the parasite and the human HepG2 cell line, identifying hits with EC50 &lt; 1 \u03bcM and a selectivity index of &gt;10. Two sesquiterpene glycosides were identified against P. falciparum, four sterols against L. mexicana, and five compounds of various scaffolds against T. brucei and T. evansi. An L. mexicana resistant line was generated for the sterol 700022, which was found to have cross-resistance to the anti-leishmanial drug miltefosine as well as to the other leishmanicidal sterols. This study highlights the potential of a temperate plant secondary metabolites as a novel source of natural products against tropical parasitic diseases.<\/jats:p>","DOI":"10.3390\/ph14030227","type":"journal-article","created":{"date-parts":[[2021,3,7]],"date-time":"2021-03-07T21:52:15Z","timestamp":1615153935000},"page":"227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Temperate Zone Plant Natural Products\u2014A Novel Resource for Activity against Tropical Parasitic Diseases"],"prefix":"10.3390","volume":"14","author":[{"given":"Hamza","family":"Hameed","sequence":"first","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7452-4518","authenticated-orcid":false,"given":"Elizabeth F. B.","family":"King","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Doleckova","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Department of Biology, Faculty of Life Sciences, University of Hradec Kr\u00e1lov\u00e9, 500 03 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Bartholomew","sequence":"additional","affiliation":[{"name":"PhytoQuest Limited, Aberystwyth SY23 3EB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackie","family":"Hollinshead","sequence":"additional","affiliation":[{"name":"PhytoQuest Limited, Aberystwyth SY23 3EB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haddijatou","family":"Mbye","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"},{"name":"MRC Unit The Gambia at LSHTM, Atlantic Boulevard, Fajara, Banjul PO Box 273, The Gambia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1432-1705","authenticated-orcid":false,"given":"Imran","family":"Ullah","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Walker","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Keele University, Staffordshire ST5 5BG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4295-6364","authenticated-orcid":false,"given":"Maria","family":"Van Veelen","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somaia Saif","family":"Abou-Akkada","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Alexandria University, Alexandria, Abis 10, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Nash","sequence":"additional","affiliation":[{"name":"PhytoQuest Limited, Aberystwyth SY23 3EB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D.","family":"Horrocks","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1537-4390","authenticated-orcid":false,"given":"Helen P.","family":"Price","sequence":"additional","affiliation":[{"name":"Centre for Applied Entomology and Parasitology, Keele University, Staffordshire ST5 5BG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,7]]},"reference":[{"key":"ref_1","unstructured":"(2020, December 02). 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