{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T09:57:16Z","timestamp":1771495036851,"version":"3.50.1"},"reference-count":109,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T00:00:00Z","timestamp":1629936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Hancornia speciosa Gomes is a tree native to Brazil and has therapeutic potential for several diseases. Ethnopharmacological surveys have reported that the plant is used as a hypoglycemic agent and to lose weight. This study aimed to evaluate the effects of the aqueous extract from H. speciosa latex (LxHs) in a zebrafish model of diabetes. The extract was evaluated through high-performance thin-layer chromatography (HTPLC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR). We then tested treatments with LxHs (500, 1000, and 1500 mg\/kg) by assessing blood glucose levels in alloxan-induced diabetic animals, and metformin was used as a control. The toxicity was evaluated through histopathology of the pancreas and biochemical assessment of serum levels of AST, ALT, creatinine, and urea. The extract was also assessed for acute toxicity through several parameters in embryos and adult animals. Finally, we performed in silico analysis through the SEA server and docking using the software GOLD. The phytochemical study showed the compounds cornoside, dihydrocornoide, and 1-O-methyl-myoinositol (bornesitol). The treatment with all doses of LxHs significantly decreased alloxan-induced hyperglycemia without any significant histological or biochemical abnormalities. No significant frequency of teratogenesis was observed in the embryos exposed to the extract, and no significant behavioral changes or deaths were observed in adult animals. In silico, the results showed a potential interaction between inositol and enzymes involved in carbohydrates\u2019 metabolism. Overall, the results show a hypoglycemic activity of the extract in vivo, with no apparent toxicity. The computational studies suggest this could be at least partially due to the presence of bornesitol, since inositols can interact with carbohydrates\u2019 enzymes.<\/jats:p>","DOI":"10.3390\/ph14090856","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T09:27:57Z","timestamp":1629970077000},"page":"856","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Hypoglycemic Activity of Aqueous Extract of Latex from Hancornia speciosa Gomes: A Study in Zebrafish and In Silico"],"prefix":"10.3390","volume":"14","author":[{"given":"Rosana","family":"Tomazi","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Rede Bionorte (Ppg-Bionorte), Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Amap\u00e1 (IFAP), Rodovia BR-210, km 03, S\/n\u2014Brasil Novo, Macap\u00e1 68909-398, AP, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"},{"name":"Laborat\u00f3rio de Farmacognosia e Fitoqu\u00edmica, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0272-1595","authenticated-orcid":false,"given":"\u00c2ngela Costa","family":"Figueira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Rede Bionorte (Ppg-Bionorte), Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Amap\u00e1 (IFAP), Rodovia BR-210, km 03, S\/n\u2014Brasil Novo, Macap\u00e1 68909-398, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Maciel","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego Quaresma","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3553-3635","authenticated-orcid":false,"given":"Gisele Cust\u00f3dio","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2977-8863","authenticated-orcid":false,"given":"Wandson Braamcamp","family":"de Souza Pinheiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Industrial, Instituto de Qu\u00edmica, Universidade Federal do Par\u00e1 (UFPA), Rua. Augusto Corr\u00eaa, Guam\u00e1, 01, Bel\u00e9m 66075-110, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2687-3921","authenticated-orcid":false,"given":"Jos\u00e9 Rodrigues","family":"Pinheiro Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Industrial, Instituto de Qu\u00edmica, Universidade Federal do Par\u00e1 (UFPA), Rua. Augusto Corr\u00eaa, Guam\u00e1, 01, Bel\u00e9m 66075-110, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1119-4248","authenticated-orcid":false,"given":"Geilson Alcantara","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Industrial, Instituto de Qu\u00edmica, Universidade Federal do Par\u00e1 (UFPA), Rua. Augusto Corr\u00eaa, Guam\u00e1, 01, Bel\u00e9m 66075-110, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8973-4902","authenticated-orcid":false,"given":"Henrique Barros","family":"de Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Medicinal, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7851-7363","authenticated-orcid":false,"given":"Lorane Izabel","family":"da Silva Hage-Melim","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Medicinal, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9504-5081","authenticated-orcid":false,"given":"Arlindo C\u00e9sar Matias","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em F\u00e1rmacos, Departamento de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Amap\u00e1 (UNIFAP), Rod. 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Juscelino Kubitschek, km 02\u2014Jardim Marco Zero, Macap\u00e1 68903-419, AP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,26]]},"reference":[{"key":"ref_1","unstructured":"Filho, V.C., and Zanchett, C.C.C. (2020). Fitoterapia Avan\u00e7ada: Uma Abordagem Qu\u00edmica, Biol\u00f3gica e Nutricional, Artmed."},{"key":"ref_2","unstructured":"Pedrosa, V.M.D. (2018). Fisiologia, Qualidade e Potencial Funcional de Frutos de Diferentes Acessos de Mangabeira (Hancornia speciosa Gomes), Universidade Federal da Para\u00edba. Trabalho de Conclus\u00e3o de Curso (Gradua\u00e7\u00e3o)-Curso de Agronomia."},{"key":"ref_3","unstructured":"(2021, March 23). Brasil, Minist\u00e9rio do Meio Ambiente\u2013MMA Relat\u00f3rio T\u00e9cnico de Monitoramento do Desmatamento no Bioma Cerrado, 2001 a 2008: Dados Revisados. 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