{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T20:33:08Z","timestamp":1782592388570,"version":"3.54.5"},"reference-count":104,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Science and Higher Education","award":["Grant No. NN 405 358737"],"award-info":[{"award-number":["Grant No. NN 405 358737"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The study was carried out on alcohol-preferring male Wistar rats. The following drugs were repeatedly (28\u00d7) administered: acamprosate (500 mg\/kg, p.o.), naltrexone (0.1 mg\/kg, i.p), and Pueraria lobata (kudzu) root extract (KU) (500 mg\/kg, p.o.) and its isoflavones: daidzin (40 mg\/kg, p.o.) and puerarin (150 mg\/kg, p.o.). Their effects on a voluntary alcohol intake were assessed. KU and alcohol were also given for 9 days in an experiment on alcohol tolerance development. Finally, total and active ghrelin levels in peripheral blood serum were measured by ELISA method. Acamprosate, naltrexone, daidzin, and puerarin, reducing the alcohol intake, caused an increase in both forms of ghrelin levels. On the contrary, though KU inhibited the alcohol intake and alcohol tolerance development, it reduced ghrelin levels in alcohol-preferring rats. The changes of ghrelin concentration could play a role as an indicator of the currently used drugs. The other effect on the KU-induced shift in ghrelin levels in the presence of alcohol requires further detailed study.<\/jats:p>","DOI":"10.3390\/ph15010025","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T01:06:54Z","timestamp":1640567214000},"page":"25","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Differential Influence of Pueraria lobata Root Extract and Its Main Isoflavones on Ghrelin Levels in Alcohol-Treated Rats"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3295-2789","authenticated-orcid":false,"given":"Micha\u0142","family":"Szulc","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2389-0689","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Kujawski","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-3078","authenticated-orcid":false,"given":"Justyna","family":"Baraniak","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0128-6680","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Kania-Dobrowolska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Kami\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-9914","authenticated-orcid":false,"given":"Agnieszka","family":"Gryszczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Czora-Poczwardowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9612-3819","authenticated-orcid":false,"given":"Hanna","family":"Winiarska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-6523","authenticated-orcid":false,"given":"Przemys\u0142aw \u0141.","family":"Miko\u0142ajczak","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pozna\u0144 University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland"},{"name":"Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"ref_1","first-page":"155","article-title":"Chronic diseases and conditions related to alcohol use","volume":"35","author":"Shield","year":"2013","journal-title":"Alcohol Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1177\/1039856220943024","article-title":"Alcohol consumption and alcohol-related problems during the COVID-19 pandemic: A narrative review","volume":"28","author":"Ramalho","year":"2020","journal-title":"Australas. 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