{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:27:48Z","timestamp":1785774468055,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T00:00:00Z","timestamp":1641945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008369","name":"Archimedes Foundation","doi-asserted-by":"publisher","award":["Development of additional analytical capabilities for Estonian Center of Analytical Chemistry, AKKI"],"award-info":[{"award-number":["Development of additional analytical capabilities for Estonian Center of Analytical Chemistry, AKKI"]}],"id":[{"id":"10.13039\/100008369","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008369","name":"Archimedes Foundation","doi-asserted-by":"publisher","award":["TK-134"],"award-info":[{"award-number":["TK-134"]}],"id":[{"id":"10.13039\/100008369","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002301","name":"Estonian Research Council","doi-asserted-by":"publisher","award":["PSG11"],"award-info":[{"award-number":["PSG11"]}],"id":[{"id":"10.13039\/501100002301","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Lyme disease (LD) is a tick-borne bacterial disease that is caused by Borrelia burgdorferi. Although acute LD is treated with antibiotics, it can develop into relapsing chronic form caused by latent forms of B. burgdorferi. This leads to the search for phytochemicals against resistant LD. Therefore, this study aimed to evaluate the activity of Dipsacus fullonum L. leaves extract (DE) and its fractions against stationary phase B. burgdorferi in vitro. DE showed high activity against stationary phase B. burgdorferi (residual viability 19.8 \u00b1 4.7%); however, it exhibited a noticeable cytotoxicity on NIH cells (viability 20.2 \u00b1 5.2%). The iridoid-glycoside fraction showed a remarkable anti-Borrelia effect and reduced cytotoxicity. The iridoid-glycoside fraction was, therefore, further purified and showed to contain two main bioactives\u2014sylvestrosides III and IV, that showed a considerable anti-Borrelia activity being the least toxic to murine fibroblast NIH\/3T3 cells. Moreover, the concentration of sylvestrosides was about 15% of DE, endorsing the feasibility of purification of the compounds from D. fullonum L. leaves.<\/jats:p>","DOI":"10.3390\/ph15010087","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T23:17:07Z","timestamp":1642029427000},"page":"87","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Extraction and Fractionation of Bioactives from Dipsacus fullonum L. Leaves and Evaluation of Their Anti-Borrelia Activity"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3489-4797","authenticated-orcid":false,"given":"Piret","family":"Saar-Reismaa","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Bragina","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"},{"name":"National Institute for Health Development, 11619 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kuhtinskaja","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3278-7947","authenticated-orcid":false,"given":"Indrek","family":"Reile","sequence":"additional","affiliation":[{"name":"National Institute of Chemical Physics and Biophysics, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2276-2925","authenticated-orcid":false,"given":"Pille-Riin","family":"Laanet","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kulp","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-1906","authenticated-orcid":false,"given":"Merike","family":"Vaher","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, 12618 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1016\/j.pain.2014.04.024","article-title":"Acute and chronic pain associated with Lyme borreliosis: Clinical characteristics and pathophysiologic mechanisms","volume":"155","author":"Zimering","year":"2014","journal-title":"Pain"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"Stanek","year":"2012","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.3389\/fneur.2018.01048","article-title":"The emerging role of microbial biofilm in lyme neuroborreliosis","volume":"9","author":"Cavallo","year":"2018","journal-title":"Front. 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