{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T09:14:14Z","timestamp":1778231654104,"version":"3.51.4"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T00:00:00Z","timestamp":1671235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Institute","award":["P30CA033572"],"award-info":[{"award-number":["P30CA033572"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The world-wide high incidence of non-alcoholic fatty liver disease (NAFLD) is of concern for its progression to insulin resistance, steatohepatitis and cardiovascular disease (CVD). The increased uptake of fatty acids in critical organs plays a major role in NAFLD progression. Male Ceacam1\u2212\/\u2212 mice that develop NAFLD, insulin resistance and CVD on normal chow are a potential model for studying the dysregulation of fatty acid uptake. [18F]fluoro-4-thia-oleate ([18F]FTO) was chosen as a fatty acid reporter because of its higher uptake and retention in the heart in an animal model of CVD. Male wild-type (WT) or Ceacam1\u2212\/\u2212 mice fasted 4\u20136 h were administered [18F]FTO i.v., and dynamic PET scans were conducted in an MR\/PET small animal imaging system along with terminal tissue biodistributions. Quantitative heart image analysis revealed significantly higher uptake at 35 min in Ceacam1\u2212\/\u2212 (6.0 \u00b1 1.0% ID\/cc) vs. WT (3.9 \u00b1 0.6% ID\/cc) mice (p = 0.006). Ex vivo heart uptake\/retention (% ID\/organ) was 2.82 \u00b1 0.45 for Ceacam1\u2212\/\u2212 mice vs. 1.66 \u00b1 0.45 for WT mice (p &lt; 0.01). Higher kidney and pancreas uptake\/retention in Ceacam1\u2212\/\u2212 was also evident, and the excretion of [18F]FTO into the duodenum was observed for both WT and Ceacam1\u2212\/\u2212 mice starting at 10 min. This study suggests that the administration of [18F]FTO as a marker of fatty acid uptake and retention may be an important tool in analyzing the effect of NAFLD on lipid dysregulation in the heart.<\/jats:p>","DOI":"10.3390\/ph15121577","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:54:13Z","timestamp":1671425653000},"page":"1577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Heart Uptake of [18F]Fluoro-4-Thia-Oleate in a Non-Alcoholic Fatty Liver Disease Mouse Model"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2398-7904","authenticated-orcid":false,"given":"Junfeng","family":"Li","sequence":"first","affiliation":[{"name":"Arthur Riggs Institute of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3911-5262","authenticated-orcid":false,"given":"Weidong","family":"Hu","sequence":"additional","affiliation":[{"name":"Arthur Riggs Institute of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangling","family":"Peng","sequence":"additional","affiliation":[{"name":"Arthur Riggs Institute of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patty","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, City of Hope National Medical Center, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fouad","family":"Kandeel","sequence":"additional","affiliation":[{"name":"Arthur Riggs Institute of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tove","family":"Olafsen","sequence":"additional","affiliation":[{"name":"Small Animal Imaging Core, Shared Resources, City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7763-770X","authenticated-orcid":false,"given":"John E.","family":"Shively","sequence":"additional","affiliation":[{"name":"Arthur Riggs Institute of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1007\/s00392-020-01709-7","article-title":"NAFLD and cardiovascular diseases: A clinical review","volume":"110","author":"Kasper","year":"2021","journal-title":"Clin. 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