{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:24:40Z","timestamp":1784899480609,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003693","name":"Korea Institute of Science and Technology","doi-asserted-by":"publisher","award":["2E31920-22-045"],"award-info":[{"award-number":["2E31920-22-045"]}],"id":[{"id":"10.13039\/501100003693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003693","name":"Korea Institute of Science and Technology","doi-asserted-by":"publisher","award":["GCU-2019-0826"],"award-info":[{"award-number":["GCU-2019-0826"]}],"id":[{"id":"10.13039\/501100003693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002631","name":"Gachon University","doi-asserted-by":"publisher","award":["2E31920-22-045"],"award-info":[{"award-number":["2E31920-22-045"]}],"id":[{"id":"10.13039\/501100002631","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002631","name":"Gachon University","doi-asserted-by":"publisher","award":["GCU-2019-0826"],"award-info":[{"award-number":["GCU-2019-0826"]}],"id":[{"id":"10.13039\/501100002631","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Kaempferol, a natural flavonoid abundantly found in plants, is known to have pharmacological properties, such as anti-inflammatory and anti-cancer effects. In this study, we investigated the antiviral effects of kaempferol against a varicella-zoster virus (VZV) clinical isolate in vitro. We found that kaempferol significantly inhibited VZV replication without exhibiting cytotoxicity. Kaempferol exerted its antiviral effect at a similar stage of the VZV life cycle as acyclovir, which inhibits VZV DNA replication. Taken together, our results suggest that kaempferol inhibits VZV infection by blocking the DNA replication stage in the viral life cycle.<\/jats:p>","DOI":"10.3390\/ph15121582","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:54:13Z","timestamp":1671425653000},"page":"1582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Kaempferol Interferes with Varicella-Zoster Virus Replication in Human Foreskin Fibroblasts"],"prefix":"10.3390","volume":"15","author":[{"given":"Subin","family":"Park","sequence":"first","affiliation":[{"name":"Department of Life Science, Gachon University, Seongnam-si 13120, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na-Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University, Seongnam-si 13120, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang Ju","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Electronic Engineering, Gachon University, Seongnam-si 13120, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4071-2331","authenticated-orcid":false,"given":"Hak Cheol","family":"Kwon","sequence":"additional","affiliation":[{"name":"Natural Product Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute, Gangneung 25451, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7900-5012","authenticated-orcid":false,"given":"Yoon-Jae","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gachon University, Seongnam-si 13120, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,19]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Varicella zoster virus infection","volume":"1","author":"Gershon","year":"2015","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Depledge, D.P., Sadaoka, T., and Ouwendijk, W.J. (2018). Molecular aspects of varicella-zoster virus latency. Viruses, 10.","DOI":"10.20944\/preprints201806.0036.v1"},{"key":"ref_3","first-page":"243","article-title":"Neurological disease produced by varicella zoster virus reactivation without rash","volume":"342","author":"Gilden","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S68","DOI":"10.1093\/infdis\/jiy403","article-title":"Immunobiology of varicella-zoster virus infection","volume":"218","author":"Laing","year":"2018","journal-title":"J. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1128\/CMR.00052-13","article-title":"Pathogenesis and current approaches to control of varicella-zoster virus infections","volume":"26","author":"Gershon","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kennedy, P.G., and Gershon, A.A. (2018). Clinical features of varicella-zoster virus infection. Viruses, 10.","DOI":"10.3390\/v10110609"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1097\/00006454-200210000-00009","article-title":"Hospitalizations for varicella in the United States, 1988 to 1999","volume":"21","author":"Galil","year":"2002","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1093\/cid\/ciy953","article-title":"The epidemiology of herpes zoster in the United States during the era of varicella and herpes zoster vaccines: Changing patterns among older adults","volume":"69","author":"Harpaz","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1016\/S1386-6532(10)70003-2","article-title":"The impact of varicella zoster virus: Chronic pain","volume":"48","author":"Opstelten","year":"2010","journal-title":"J. Clin. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Holmes, S.J., Reef, S.E., Hadler, S.C., Williams, W.W., and Wharton, M. (1996). Prevention of Varicella; Recommendations of the Advisory Committee on Immunization Practices, Centers for Disease Control and Prevention.","DOI":"10.1093\/infdis\/174.Supplement_3.S342"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-1-4684-5853-4_25","article-title":"Acyclovir: The past ten years","volume":"278","author":"Whitley","year":"1990","journal-title":"Immunobiol. Prophyl. Hum. Herpesvirus Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1097\/QCO.0000000000000211","article-title":"Herpes simplex virus and varicella zoster virus: Recent advances in therapy","volume":"28","author":"De","year":"2015","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.trsl.2011.07.002","article-title":"Acyclovir-induced nephrotoxicity: The role of the acyclovir aldehyde metabolite","volume":"158","author":"Gunness","year":"2011","journal-title":"Transl. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0166-3542(95)00066-6","article-title":"Valacyclovir: A review of its antiviral activity, pharmacokinetic properties, and clinical efficacy","volume":"28","author":"Beutner","year":"1995","journal-title":"Antivir. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1093\/cid\/cis879","article-title":"Persistence and antiviral resistance of varicella zoster virus in hematological patients","volume":"56","author":"Vermont","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Andrei, G., and Snoeck, R. (2011). Advances and perspectives in the management of varicella-zoster virus infections. Molecules, 26.","DOI":"10.3390\/molecules26041132"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Imran, M., Salehi, B., Sharifi-Rad, J., Aslam Gondal, T., Saeed, F., Imran, A., Shahbaz, M., Tsouh Fokou, P.V., Umair Arshad, M., and Khan, H. (2019). Kaempferol: A key emphasis to its anticancer potential. Molecules, 24.","DOI":"10.3390\/molecules24122277"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Alam, W., Khan, H., Shah, M.A., Cauli, O., and Saso, L. (2020). Kaempferol as a dietary anti-inflammatory agent: Current therapeutic standing. Molecules, 25.","DOI":"10.3390\/molecules25184073"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.foodchem.2007.11.053","article-title":"Flavonols (Kaempeferol, Quercetin, Myricetin) Contents of Selected Fruits, Vegetables and Medicinal Plants","volume":"108","author":"Sultana","year":"2008","journal-title":"Food. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.3389\/fmicb.2021.736780","article-title":"Flavonoid library screening reveals kaempferol as a potential antiviral agent against african swine fever virus","volume":"12","author":"Arabyan","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7141","DOI":"10.1016\/j.bmc.2008.06.049","article-title":"Structure\u2013activity relationship of flavonoids as influenza virus neuraminidase inhibitors and their in vitro anti-viral activities","volume":"16","author":"Liu","year":"2008","journal-title":"Bioorganic Med. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhang, T., Wu, Z., Du, J., Hu, Y., Liu, L., Yang, F., and Jin, Q. (2012). Anti-japanese-encephalitis-viral effects of kaempferol and daidzin and their rna-binding characteristics. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0030259"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1007\/BF02978215","article-title":"Antiherpetic activities of flavonoids against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro","volume":"28","author":"Lyu","year":"2005","journal-title":"Arch. Pharmacal Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13578-016-0086-6","article-title":"Type i interferon inhibits varicella-zoster virus replication by interfering with the dynamic interaction between mediator and ie62 within replication compartments","volume":"6","author":"Ku","year":"2016","journal-title":"Cell Biosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"12198","DOI":"10.1128\/JVI.01280-12","article-title":"Identification of a varicella-zoster virus replication inhibitor that blocks capsid assembly by interacting with the floor domain of the major capsid protein","volume":"86","author":"Inoue","year":"2012","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1021\/jf100332w","article-title":"Flavonoids and Phenolic Compounds from Rosmarinus Officinalis","volume":"58","author":"Bai","year":"2010","journal-title":"J. Agric. Food. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Dabeek, W.M., and Marra, M.V. (2019). Dietary Quercetin and Kaempferol: Bioavailability and Potential Cardiovascular-Related Bioactivity in Humans. Nutrients, 11, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31557798.","DOI":"10.3390\/nu11102288"},{"key":"ref_28","first-page":"463","article-title":"In vitro anti-hiv-1 activities of kaempferol and kaempferol-7-o-glucoside isolated from securigera securidaca","volume":"9","author":"Behbahani","year":"2014","journal-title":"Res. Pharm. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.3892\/ijo.2013.1909","article-title":"Kaempferol induces autophagy through ampk and akt signaling molecules and causes g2\/m arrest via downregulation of cdk1\/cyclin b in sk-hep-1 human hepatic cancer cells","volume":"42","author":"Huang","year":"2013","journal-title":"Int. J. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00505","DOI":"10.1128\/JVI.00505-19","article-title":"Progeny varicella-zoster virus capsids exit the nucleus but never undergo secondary envelopment during autophagic flux inhibition by bafilomycin a1","volume":"93","author":"Girsch","year":"2019","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.virol.2016.06.017","article-title":"Analysis of single nucleotide polymorphism among varicella-zoster virus and identification of vaccine-specific sites","volume":"496","author":"Jeon","year":"2016","journal-title":"Virology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep07237","article-title":"A dual character of flavonoids in influenza a virus replication and spread through modulating cell-autonomous immunity by mapk signaling pathways","volume":"4","author":"Dong","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12906-017-1941-7","article-title":"Inhibition of human cytomegalovirus immediate-early gene expression and replication by the ethyl acetate (etoac) fraction of elaeocarpus sylvestris in vitro","volume":"17","author":"Bae","year":"2017","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3149","DOI":"10.1128\/IAI.01237-10","article-title":"Suppressed Induction of Proinflammatory Cytokines by a Unique Metabolite Produced by Vibrio Cholerae o1 el tor Biotype in Cultured Host Cells","volume":"79","author":"Bari","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.antiviral.2017.06.018","article-title":"1, 2, 3, 4, 6-penta-o-galloyl-ss-d-glucose, a bioactive compound in elaeocarpus sylvestris extract, inhibits varicella-zoster virus replication","volume":"144","author":"Bae","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kim, N.E., Kim, D.K., and Song, Y.J. (2021). SARS-CoV-2 Nonstructural Proteins 1 and 13 Suppress Caspase-1 and the nlrp3 Inflammasome Activation. Microorganisms, 9, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33652815.","DOI":"10.3390\/microorganisms9030494"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/15\/12\/1582\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:43:46Z","timestamp":1760147026000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/15\/12\/1582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,19]]},"references-count":36,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ph15121582"],"URL":"https:\/\/doi.org\/10.3390\/ph15121582","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,19]]}}}