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The results of the in vivo study indicate that derivatives of m-anisidine (4) and N-methyl tryptophan methyl ester (7) showed the most potent anti-inflammatory activity four hours after injection of carrageenan, with the percentage of inhibition of 54.01% and 54.12%, respectively. In vitro assays of COX-2 inhibition demonstrated that none of the tested compounds achieved 50% inhibition at concentrations lower than 100 \u00b5M. On the other hand, the aromatic amine derivatives (1\u20135) accomplished significant inhibition of 5-LOX, and the lowest IC50 value was observed for compound 4 (0.30 \u03bcM). High anti-edematous activity of compound 4 in the rat paw edema model, together with potent inhibition of 5-LOX, highlight this compound as a promising anti-inflammatory agent.<\/jats:p>","DOI":"10.3390\/ph16050666","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T09:54:53Z","timestamp":1682675693000},"page":"666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Synthesis and Investigation of Anti-Inflammatory Activity of New Thiourea Derivatives of Naproxen"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4560-2630","authenticated-orcid":false,"given":"Nikola","family":"Nedeljkovi\u0107","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, 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