{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T13:02:46Z","timestamp":1769605366645,"version":"3.49.0"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T00:00:00Z","timestamp":1684368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INSERM and R\u00e9gion Pays de la Loire (PROVASC project)","award":["n\u00b0 00909.01"],"award-info":[{"award-number":["n\u00b0 00909.01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Vascular aging is linked to reduce NO bioavailability, endothelial dysfunction, oxidative stress, and inflammation. We previously showed that a 4-week treatment of middle-aged Wistar rats (MAWRs, 46 weeks old) with Moringa oleifera seed powder (MOI, 750 mg\/kg\/day) improved vascular function. Here, we investigated the involvement of SIRT1 in MOI-induced vascular improvement. MAWRs were treated with a standard or MOI-containing diet. Young rats (YWR, 16 weeks old) were the controls and received a standard diet. The hearts and aortas were harvested to evaluate SIRT1 and FOXO1 expression via Western blot and\/or immunostaining, SIRT1 activity via a fluorometric assay, and oxidative stress using the DHE fluorescent probe. In the hearts and aortas, SIRT1 expression, reduced in MAWRs compared to YWRs, was enhanced in MOI MAWRs. In the hearts, SIRT1 activity did not differ between YWRs and MAWRs, whereas it was increased in MOI MAWRs compared with them. In the aortas, SIRT1 activity decreased in MAWRs, and it was similar in the MOI MAWRs and YWRs. FOXO1 expression increased in the nuclei of MAWR aortas compared to YWR and was reversed in MOI MAWRs. Interestingly, MOI treatment normalized oxidative stress enhanced in MAWRs, in both the heart and aorta. These results demonstrate the protective role of MOI against cardiovascular dysfunction due to aging via enhanced SIRT1 function and subsequently reduced oxidative stress.<\/jats:p>","DOI":"10.3390\/ph16050761","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T07:35:50Z","timestamp":1684395350000},"page":"761","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6964-2739","authenticated-orcid":false,"given":"Valeria","family":"Conti","sequence":"first","affiliation":[{"name":"Department of Medicine Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, S. Allende, 84081 Baronissi, SA, Italy"},{"name":"Clinical Pharmacology Unit, San Giovanni di Dio e Ruggi d\u2019Aragona University Hospital, San Leonardo 1, 84131 Salerno, SA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0367-3564","authenticated-orcid":false,"given":"Joseph Iharinjaka","family":"Randriamboavonjy","sequence":"additional","affiliation":[{"name":"INSERM, Universit\u00e9 de Nantes, CHU Nantes, CNRS, L\u2019Institut du Thorax, CEDEX 01, F-44000 Nantes, France"},{"name":"Laboratoire d\u2019\u00c9valuation Pharmaco Clinique (LEPC), Institut Malgache de Recherches Appliqu\u00e9es (IMRA) Fondation Albert et Suzanne Rakoto-Ratsimamanga (FASRR), Avarabohitra Itaosy, Antananarivo 102, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herintsoa","family":"Rafatro","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019\u00c9valuation Pharmaco Clinique (LEPC), Institut Malgache de Recherches Appliqu\u00e9es (IMRA) Fondation Albert et Suzanne Rakoto-Ratsimamanga (FASRR), Avarabohitra Itaosy, Antananarivo 102, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4521-0453","authenticated-orcid":false,"given":"Valentina","family":"Manzo","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Unit, San Giovanni di Dio e Ruggi d\u2019Aragona University Hospital, San Leonardo 1, 84131 Salerno, SA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0042-2161","authenticated-orcid":false,"given":"Jessica","family":"Dal Col","sequence":"additional","affiliation":[{"name":"Department of Medicine Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, S. Allende, 84081 Baronissi, SA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8235-9118","authenticated-orcid":false,"given":"Amelia","family":"Filippelli","sequence":"additional","affiliation":[{"name":"Department of Medicine Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, S. Allende, 84081 Baronissi, SA, Italy"},{"name":"Clinical Pharmacology Unit, San Giovanni di Dio e Ruggi d\u2019Aragona University Hospital, San Leonardo 1, 84131 Salerno, SA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3441-889X","authenticated-orcid":false,"given":"Graziamaria","family":"Corbi","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, NA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2153-4357","authenticated-orcid":false,"given":"Angela","family":"Tesse","sequence":"additional","affiliation":[{"name":"INSERM, Universit\u00e9 de Nantes, CHU Nantes, CNRS, L\u2019Institut du Thorax, CEDEX 01, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rizki, G., Iwata, T.N., Li, J., Riedel, C.G., Picard, C.L., Jan, M., Murphy, C.T., and Lee, S.S. 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