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Therapeutic drug monitoring is crucial to ensuring the efficacy and safety of antifungal agents owing to their pharmacokinetic variability. In the present study, we developed and validated a quantitative method for the simultaneous detection of seven commonly used antifungal drugs (amphotericin B, isavuconazole, voriconazole, fluconazole, posaconazole, caspofungin, and micafungin) using liquid chromatography-tandem mass spectrometry. Methanol (containing 0.1% formic acid) was used for protein precipitation and only 50 \u03bcL of serum was required for the analysis. Chromatographic separation was conducted using a Waters Acquity UPLC C8 column, and one stable isotope-labeled agent and two analogs were used as internal standards. The calibration curves ranged from 0.1 to 50 \u03bcg\/mL for all agents, and the correlation coefficient (R2) for all calibration curves was above 0.9835. The intra-day precision (1.2\u201311.2%), inter-day precision (2.4\u201313.2%), and mean bias values (\u221210.9 to 13.6%) were within an acceptable range of \u00b115%. Successful implementation of the developed method in clinical practice would facilitate the effective monitoring of these antifungal agents.<\/jats:p>","DOI":"10.3390\/ph16111537","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:07:45Z","timestamp":1698667665000},"page":"1537","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Simultaneous Quantification of Seven Antifungal Agents in Human Serum Using Liquid Chromatography-Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"16","author":[{"given":"Wenjing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Zhongnan Hospital of Wuhan University, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlong","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Clinical Trial Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hankun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and Update of the Consensus Definitions of Invasive Fungal Disease from the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin. 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