{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T19:05:58Z","timestamp":1788030358871,"version":"build-2784847793"},"reference-count":61,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T00:00:00Z","timestamp":1703808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Austrian Science Fund (FWF)","award":["P-27814-B22"],"award-info":[{"award-number":["P-27814-B22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Pimenta dioica L. is one the most recognized species with diverse biological activities. In this study, in vitro activity and in vivo efficacy of essential oil from P. dioica (EO-Pd) was evaluated. The main compound was also included in the animal studies and its in silico prediction related to biological activities, molecular ligands, drug likeness, and ADME (absorption, distribution, metabolism, and excretion) properties are listed. The chemical composition analyzed by GC-MS retrieved 45 components, which the most abundant compound was the eugenol (80.1%). The EO-Pd was able to inhibit the growth of L. amazonensis (IC50 = 9.7 \u00b1 0.7 and 11.3 \u00b1 2.1 \u00b5g\/mL, promastigotes and amastigotes, respectively). The cytotoxicity assay showed a CC50 of 104.5 \u00b1 0.9 \u00b5g\/mL and a selectivity index of 9. In the model of cutaneous leishmaniasis in BALB\/c mice, the effect of EO-Pd and eugenol was observed after treatment at 30 mg\/kg by intralesional route with 5 administrations every 4 days. In the in silico predictions, some targets that justified the antileishmanial activity of eugenol and good drug like properties for this compound, were obtained. This study showed for first time the potential of EO-Pd to inhibit L. amazonensis, which could be linked to the activity of major compound eugenol.<\/jats:p>","DOI":"10.3390\/ph17010064","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T11:19:21Z","timestamp":1703848761000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Eugenol-Rich Essential Oil from Pimenta dioica: In Vitro and In Vivo Potentialities against Leishmania amazonensis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1958-809X","authenticated-orcid":false,"given":"Lianet","family":"Monzote","sequence":"first","affiliation":[{"name":"Parasitology Department, Center of Research, Diagnostic and Reference, Institute of Tropical Medicine \u201cPedro Kouri\u201d, Havana 17100, Cuba"},{"name":"Research Network Natural Products against Neglected Diseases (ResNetNPND), 48149 Munster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0708-6789","authenticated-orcid":false,"given":"Laura","family":"Mach\u00edn","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 13600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adiel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Parasitology Department, Center of Research, Diagnostic and Reference, Institute of Tropical Medicine \u201cPedro Kouri\u201d, Havana 17100, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Scull","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 13600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamilet I.","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 13600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-1074","authenticated-orcid":false,"given":"Prabodh","family":"Satyal","sequence":"additional","affiliation":[{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-0201","authenticated-orcid":false,"given":"Lars","family":"Gille","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Department of Biomedical Sciences, University of Veterinary Medicine, Veterin\u00e4rplatz 1, A-1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-0528","authenticated-orcid":false,"given":"William N.","family":"Setzer","sequence":"additional","affiliation":[{"name":"Research Network Natural Products against Neglected Diseases (ResNetNPND), 48149 Munster, Germany"},{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"},{"name":"Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"El-Shemy, H. (2018). Potential of Essential Oils, IntechOpen Limited.","DOI":"10.5772\/intechopen.69939"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ismail, M.M., Samir, R., Saber, F.R., Ahmed, S.R., and Farag, M.A. (2020). Pimenta Oil as A Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition. Antibiotics, 9.","DOI":"10.3390\/antibiotics9100679"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1080\/14786419.2012.668691","article-title":"Comparative chemical composition and in vitro antioxidant activities of essential oil isolated from the leaves of Cinnamomum tamala and Pimenta dioica","volume":"27","author":"Rani","year":"2013","journal-title":"Nat. Prod. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lorenzo-Leal, A.C., Palou, E., L\u00f3pez-Malo, A., and Bach, H. (2019). Antimicrobial, Cytotoxic, and Anti-Inflammatory Activities of Pimenta dioica and Rosmarinus officinalis Essential Oils. Biomed. Res. Int., 2019.","DOI":"10.1155\/2019\/1639726"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s00436-010-2069-6","article-title":"Acaricidal effect and chemical composition of essential oils extracted from Cuminum cyminum, Pimenta dioica and Ocimum basilicum against the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)","volume":"108","year":"2011","journal-title":"Parasitol. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Narayanankutty, A., Kuttithodi, A.M., Alfarhan, A., Rajagopal, R., and Barcelo, D. (2021). Chemical Composition, Insecticidal and Mosquito Larvicidal Activities of Allspice (Pimenta dioica) Essential Oil. Molecules, 26.","DOI":"10.3390\/molecules26216698"},{"key":"ref_7","first-page":"275","article-title":"Nematicidal Activity of Plant Essential Oils and Components from Ajowan (Trachyspermum ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) Essential Oils Against Pine Wood Nematode (Bursaphelenchus Xylophilus)","volume":"39","author":"Park","year":"2007","journal-title":"J. Nematol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.2174\/138945012804545641","article-title":"Medicinal properties of the Jamaican pepper plant Pimenta dioica and Allspice","volume":"13","author":"Zhang","year":"2012","journal-title":"Curr. Drug Targets."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1099\/jmm.0.064709-0","article-title":"Apoptosis-like death in Leishmania donovani promastigotes induced by eugenol-rich oil of Syzygium aromaticum","volume":"63","author":"Islamuddin","year":"2014","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Le, T.B., Beaufay, C., Nghiem, D.T., Mingeot-Leclercq, M.P., and Quetin-Leclercq, J. (2017). In Vitro Anti-Leishmanial Activity of Essential Oils Extracted from Vietnamese Plants. Molecules, 22.","DOI":"10.3390\/molecules22071071"},{"key":"ref_11","first-page":"61","article-title":"Field-Deployable Treatments for Leishmaniasis: Intrinsic Challenges, Recent Developments and Next Steps","volume":"20","author":"Markle","year":"2023","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"579891","DOI":"10.3389\/fchem.2020.579891","article-title":"Use of Natural Products in Leishmaniasis Chemotherapy: An Overview","volume":"8","author":"Gervazoni","year":"2020","journal-title":"Front. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cohen, A., and Azas, N. (2021). Challenges and Tools for In Vitro Leishmania Exploratory Screening in the Drug Development Process: An Updated Review. Pathogens, 10.","DOI":"10.3390\/pathogens10121608"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6587150","DOI":"10.1155\/2019\/6587150","article-title":"Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses","volume":"2019","author":"Luna","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1017\/S0031182020001304","article-title":"Effect of essential oils on Leishmania amazonensis: A systematic review","volume":"147","author":"Oyama","year":"2020","journal-title":"Parasitology."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"303","DOI":"10.15517\/rbt.v63i1.14580","article-title":"Leaf and fruit essential oil compositions of Pimenta guatemalensis (Myrtaceae) from Costa Rica","volume":"63","author":"Chaverri","year":"2015","journal-title":"Rev. Biol. Trop."},{"key":"ref_17","unstructured":"Morton, J.F. (1981). Atlas of Medicinal Plants of Middle America: Bahamas to Yucatan, Charles C Thomas Pub Ltd."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0378-8741(03)00156-9","article-title":"Screening of antimutagenicity via antioxidant activity in Cuban medicinal plants","volume":"87","author":"Ramos","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/j.sjbs.2018.05.002","article-title":"Antiglycation, comparative antioxidant potential, phenolic content and yield variation of essential oils from 19 exotic and endemic medicinal plants","volume":"26","author":"Mahomoodally","year":"2019","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_20","first-page":"292","article-title":"Chemical composition and antifungal properties of commercial essential oils against the maize phytopathogenic fungus Fusarium verticillioides","volume":"53","author":"Brito","year":"2021","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1111\/nph.12526","article-title":"Explaining intraspecific diversity in plant secondary metabolites in an ecological context","volume":"201","author":"Moore","year":"2014","journal-title":"New Phytol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1023\/A:1013386921596","article-title":"Regulation of essential oil production in plants","volume":"34","author":"Sangwan","year":"2001","journal-title":"Plant Growth Regul."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6250","DOI":"10.1016\/j.bmc.2014.08.020","article-title":"Thymol and eugenol derivatives as potential antileishmanial agents","volume":"22","author":"Bevilaqua","year":"2014","journal-title":"Bioorg. Med. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s40409-018-0164-9","article-title":"Neolignans isolated from twigs of Nectandra leucantha Ness & Mart (Lauraceae) displayed in vitro antileishmanial activity","volume":"24","author":"Grecco","year":"2018","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hughes, K., Le, T.B., Van Der Smissen, P., Tyteca, D., Mingeot-Leclercq, M.P., and Quetin-Leclercq, J. (2023). The Antileishmanial Activity of Eugenol Associated with Lipid Storage Reduction Rather Than Membrane Properties Alterations. Molecules, 28.","DOI":"10.3390\/molecules28093871"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ijpddr.2019.06.003","article-title":"Route map for the discovery and pre-clinical development of new drugs and treatments for cutaneous leishmaniasis","volume":"11","author":"Caridha","year":"2019","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"ref_27","unstructured":"Pan American Health Organization (2018). Leishmaniasis in the Americas: Treatment Recommendations, PAHO."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8965","DOI":"10.1007\/s00253-020-10856-w","article-title":"Recent advances and new strategies on leishmaniasis treatment","volume":"104","author":"Roatt","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_29","first-page":"1799","article-title":"Chemistry and leishmanicidal activity of the essential oil from Artemisia absinthium from Cuba","volume":"9","author":"Monzote","year":"2014","journal-title":"Nat. Prod. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Monzote, L., Scherbakov, A.M., Scull, R., Satyal, P., Cos, P., Shchekotikhin, A.E., Gille, L., and Setzer, W.N. (2020). Essential Oil from Melaleuca leucadendra: Antimicrobial, Antikinetoplastid, Antiproliferative and Cytotoxic Assessment. Molecules, 25.","DOI":"10.3390\/molecules25235514"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1002\/ptr.5869","article-title":"Chemical Characterization, Antileishmanial Activity, and Cytotoxicity Effects of the Essential Oil from Leaves of Pluchea carolinensis (Jacq.) G. Don. (Asteraceae)","volume":"31","author":"Scull","year":"2017","journal-title":"Phytother. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"108269","DOI":"10.1016\/j.exppara.2022.108269","article-title":"Eugenol derivatives with 1,2,3-triazole moieties: Oral treatment of cutaneous leishmaniasis and a quantitative structure-activity relationship model for their leishmanicidal activity","volume":"238","author":"Teixeira","year":"2022","journal-title":"Exp. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nrd4683","article-title":"Hit and lead criteria in drug discovery for infectious diseases of the developing world","volume":"14","author":"Katsuno","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cheuka, P.M., Mayoka, G., Mutai, P., and Chibale, K. (2016). The Role of Natural Products in Drug Discovery and Development against Neglected Tropical Diseases. Molecules, 22.","DOI":"10.3390\/molecules22010058"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/j.amjcard.2008.02.007","article-title":"Natural Products as a Robust Source of New Drugs and Drug Leads: Past Successes and Present Day Issues","volume":"101","author":"Rishton","year":"2008","journal-title":"Am. J. Cardiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1080\/1040841X.2017.1295225","article-title":"Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint","volume":"43","author":"Marchese","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_37","first-page":"131","article-title":"Allylpolyalkoxybenzene Inhibitors of Galactonolactone Oxidase from Trypanosoma cruzi","volume":"88","author":"Chudin","year":"2023","journal-title":"Biochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"42717","DOI":"10.1038\/srep42717","article-title":"SwissADME: A free web tool to evaluate the pharmacokinetics, drug-likeness, and medicinal chemistry friendliness of small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"W357","DOI":"10.1093\/nar\/gkz382","article-title":"SwissTargetPrediction: Updated data and new features for efficient prediction of protein targets of small molecules","volume":"47","author":"Daina","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"207","DOI":"10.4103\/japtr.japtr_376_22","article-title":"Dipeptidyl peptidase IV inhibition of phytocompounds from Artocarpus champeden (Lour.) Stokes: In silico molecular docking study and ADME-Tox prediction approach","volume":"13","author":"Supandi","year":"2022","journal-title":"J. Adv. Pharm. Technol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1007\/s11030-022-10491-9","article-title":"Natural flavonoids effectively block the CD81 receptor of hepatocytes and inhibit HCV infection: A computational drug development approach","volume":"27","author":"Dey","year":"2023","journal-title":"Mol. Divers"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Foudah, A.I., Alqarni, M.H., Alam, A., Salkini, M.A., Ross, S.A., and Yusufoglu, H.S. (2022). Phytochemical Screening, In Vitro and In Silico Studies of Volatile Compounds from Petroselinum crispum (Mill) Leaves Grown in Saudi Arabia. Molecules, 27.","DOI":"10.3390\/molecules27030934"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Pontes, K.A.O., Silva, L.S., Santos, E.C., Pinheiro, A.S., Teixeira, D.E., Peruchetti, D.B., Silva-Aguiar, R.P., Wendt, C.H.C., Miranda, K.R., and Coelho-de-Souza, A.N. (2021). Eugenol disrupts Plasmodium falciparum intracellular development during the erythrocytic cycle and protects against cerebral malaria. Biochim. Biophys. Acta Gen. Subj., 1865.","DOI":"10.1016\/j.bbagen.2020.129813"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1177\/15353702231151976","article-title":"Eugenol alleviates neuronal damage via inhibiting inflammatory process against pilocarpine-induced status epilepticus","volume":"248","author":"Zhu","year":"2023","journal-title":"Exp. Biol. Med."},{"key":"ref_45","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing. [4th ed.]."},{"key":"ref_46","unstructured":"Satyal, P. (2015). Development of GC-MS Database of Essential Oil Components by the Analysis of Natural Essential Oils and Synthetic Compounds and Discovery of Biologically Active Novel Chemotypes in Essential Oils. [Ph.D. Thesis, University of Alabama in Huntsville]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e2022125","DOI":"10.1590\/1981-6723.12522","article-title":"Allspice (Pimenta Dioica Lindl) leaves essential oil as a potential antioxidant and antimicrobial source for use in mechanically deboned poultry meat","volume":"26","author":"Andrade","year":"2023","journal-title":"Braz. J. Food Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"168","DOI":"10.62773\/jcocs.v3i3.183","article-title":"Essential oil composition of allspice [Pimenta dioica (L.) Merill.] leaf from the Western Ghats, India","volume":"3","author":"Ashokkumar","year":"2022","journal-title":"J. Curr. Opin. Crop. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"207","DOI":"10.37360\/blacpma.22.21.2.12","article-title":"Chemical composition and larvicidal activity of the essential oil of Pimenta dioica leaves","volume":"21","author":"Gomes","year":"2022","journal-title":"Bol. Latinoam. Caribe. Plant Med. Aromat."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1080\/10412905.2005.9698985","article-title":"Comparative Analysis of Supercritical CO2 Extract and Oil of Pimenta dioica Leaves","volume":"17","author":"Porcedda","year":"2005","journal-title":"J. Essent. Oil Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"242","DOI":"10.37885\/220308251","article-title":"Comparative chemical evaluation and antibacterial activity of Pimenta dioica essential oil obtained by different extraction methods","volume":"3","author":"Marques","year":"2022","journal-title":"Open Sci. Res. III"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"M\u00e9rida-Reyes, M.S., Mu\u00f1oz-Wug, M.A., Oliva-Hern\u00e1ndez, B.E., Gait\u00e1n-Fern\u00e1ndez, I.C., Simas, D.L.R., Ribeiro da Silva, A.J., and P\u00e9rez-Sabino, J.F. (2020). Composition and Antibacterial Activity of the Essential Oil from Pimenta dioica (L.) Merr. from Guatemala. Medicines, 7.","DOI":"10.3390\/medicines7100059"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1007\/s13197-020-04756-0","article-title":"Mechanism of antioxidant and antifungal properties of Pimenta dioica (L.) leaf essential oil on Aspergillus flavus","volume":"58","author":"Sarathambal","year":"2021","journal-title":"J. Food Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5247","DOI":"10.1039\/D1FO00408E","article-title":"Pimenta dioica and Pimenta racemosa: GC-based metabolomics for the assessment of seasonal and organ variation in their volatile components, in silico and in vitro cytotoxic activity estimation","volume":"12","author":"Youssef","year":"2021","journal-title":"Food Funct."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1080\/10412905.1996.9700628","article-title":"Chemical Composition of the Leaf Oil of Pimenta dioica L. from Cuba","volume":"8","author":"Pino","year":"1996","journal-title":"J. Essent. Oil Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/AAC.43.5.1234","article-title":"Selective effect of 2\u2032,6\u2032-dihydroxy-4\u2032-methoxychalcone isolated from Piper aduncum on Leishmania amazonensis","volume":"43","author":"Caio","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/AAC.45.5.1349-1354.2001","article-title":"Antileishmanial activity of an indole alkaloid from Peschiera australis","volume":"45","author":"Delorenzi","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0022-1759(93)90088-O","article-title":"An improve MTT assay","volume":"157","author":"Sladowski","year":"1993","journal-title":"J. Immunol. Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.phymed.2014.03.002","article-title":"Antileishmanial activity of essential oil from Chenopodium ambrosioides and its main components against experimental cutaneous leishmaniasis in BALB\/c mice","volume":"21","author":"Monzote","year":"2014","journal-title":"Phytomedicine"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1128\/AAC.39.9.2167","article-title":"Culture microtitration: A sensitive method for quantifying Leishmania infantum in tissues of infected mice","volume":"39","author":"Buffet","year":"1995","journal-title":"Antimicrob Agents Chemother."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1080\/03610918.2014.931971","article-title":"Overview of Friedman\u2019s Test and Post-hoc Analysis","volume":"44","author":"Pereira","year":"2015","journal-title":"Commun. Stat. Simul. Comput."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/1\/64\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:44:23Z","timestamp":1760132663000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/1\/64"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,29]]},"references-count":61,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["ph17010064"],"URL":"https:\/\/doi.org\/10.3390\/ph17010064","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,29]]}}}