{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:50:41Z","timestamp":1790905841843,"version":"4.1.0"},"reference-count":85,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T00:00:00Z","timestamp":1706313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities","award":["FZSM-2022-0017"],"award-info":[{"award-number":["FZSM-2022-0017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Plant extracts are in the focus of the pharmaceutical industry as potential antimicrobials for oral care due to their high antimicrobial activity coupled with low production costs and safety for eukaryotic cells. Here, we show that the extract from Hop (Humulus lupulus L.) exhibits antimicrobial activity against Staphylococcus aureus and Streptococci in both planktonic and biofilm-embedded forms. An extract was prepared by acetone extraction from hop infructescences, followed by purification and solubilization of the remaining fraction in ethanol. The effect of the extract on S. aureus (MSSA and MRSA) was comparable with the reference antibiotics (amikacin, ciprofloxacin, and ceftriaxone) and did not depend on the bacterial resistance to methicillin. The extract also demonstrated synergy with amikacin on six S. aureus clinical isolates, on four of six isolates with ciprofloxacin, and on three of six isolates with ceftriaxone. On various Streptococci, while demonstrating lower antimicrobial activity, an extract exhibited a considerable synergistic effect in combination with two of three of these antibiotics, decreasing their MIC up to 512-fold. Moreover, the extract was able to penetrate S. aureus and S. mutans biofilms, leading to almost complete bacterial death within them. The thin-layer chromatography and LC-MS of the extract revealed the presence of prenylated flavonoids (2\u2032,4\u2032,6\u2032,4-tetrahydroxy-3\u2032-geranylchalcone) and acylphloroglucides (cohumulone, colupulone, humulone, and lupulone), apparently responsible for the observed antimicrobial activity and ability to increase the efficiency of antibiotics. Taken together, these data suggest an extract from H. lupulus as a promising antimicrobial agent for use both as a solely antiseptic and to potentiate conventional antimicrobials.<\/jats:p>","DOI":"10.3390\/ph17020162","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T04:57:07Z","timestamp":1706504227000},"page":"162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The Antimicrobial Potential of the Hop (Humulus lupulus L.) Extract against Staphylococcus aureus and Oral Streptococci"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-5554","authenticated-orcid":false,"given":"Alyona","family":"Khaliullina","sequence":"first","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyona","family":"Kolesnikova","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leysan","family":"Khairullina","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Morgatskaya","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilyara","family":"Shakirova","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Patov","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, FRC \u201cKomi Scientific Centre\u201d, Ural Branch of the Russian Academy of Sciences, 167000 Syktyvkar, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Nekrasova","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, FRC \u201cKomi Scientific Centre\u201d, Ural Branch of the Russian Academy of Sciences, 167000 Syktyvkar, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0356-5651","authenticated-orcid":false,"given":"Mikhail","family":"Bogachev","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Research Centre, St. Petersburg Electrotechnical University, 5 Professor Popov Street, 197022 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Kurkin","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Samara State Medical University, 443079 Samara, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9881-0559","authenticated-orcid":false,"given":"Elena","family":"Trizna","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7195-1557","authenticated-orcid":false,"given":"Airat","family":"Kayumov","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Li, X., Liu, Y., Yang, X., Li, C., and Song, Z. (2022). The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.895537"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41579-018-0089-x","article-title":"The oral microbiota: Dynamic communities and host interactions","volume":"16","author":"Lamont","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13075-019-1808-z","article-title":"Association between severity of periodontitis and clinical activity in rheumatoid arthritis patients: A case-control study","volume":"21","author":"Dadlani","year":"2019","journal-title":"Arthritis Res. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1600-0757.1994.tb00019.x","article-title":"The bacteria of periodontal diseases","volume":"5","author":"Moore","year":"1994","journal-title":"Periodontology 2000"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1128\/AEM.72.4.2837-2848.2006","article-title":"Molecular characterization of subject-specific oral microflora during initial colonization of enamel","volume":"72","author":"Diaz","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1600-0757.2006.00187.x","article-title":"Bacterial interactions and successions during plaque development","volume":"42","author":"Kolenbrander","year":"2006","journal-title":"Periodontology 2000"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"38309","DOI":"10.1038\/srep38309","article-title":"Deep sequencing of the 16S ribosomal RNA of the neonatal oral microbiome: A comparison of breast-fed and formula-fed infants","volume":"6","author":"Sweeney","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1177\/0022034519877386","article-title":"Precision Reengineering of the Oral Microbiome for Caries Management","volume":"30","author":"Baker","year":"2019","journal-title":"Adv. Dent. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1177\/0022034518790941","article-title":"Association between oral candida and bacteriome in children with severe ECC","volume":"97","author":"Xiao","year":"2018","journal-title":"J. Dent. Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Shen, Y., Yu, F., Qiu, L., Gao, M., Xu, P., Zhang, L., Liao, X., Wang, M., Hu, X., and Sun, Y. (2023). Ecological influence by colonization of fluoride-resistant Streptococcus mutans in oral biofilm. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.1106392"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, J.S., Chu, C.-H., and Yu, O.Y. (2022). Oral microbiome and dental caries development. Dent. J., 10.","DOI":"10.3390\/dj10100184"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1590\/S0103-64402006000300009","article-title":"Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans","volume":"17","author":"Vasconcelos","year":"2006","journal-title":"Braz. Dent. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1128\/AEM.02681-15","article-title":"Sucrose- and Fructose-Specific Effects on the Transcriptome of Streptococcus mutans, as Determined by RNA Sequencing","volume":"82","author":"Zeng","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Guo, L., Hu, W., He, X., Lux, R., McLean, J., and Shi, W. (2013). Investigating acid production by Streptococcus mutans with a surface-displayed pH-sensitive green fluorescent protein. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057182"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"32841","DOI":"10.1038\/srep32841","article-title":"Simultaneous spatiotemporal mapping of in situ pH and bacterial activity within an intact 3D microcolony structure","volume":"6","author":"Hwang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1177\/0022034517736496","article-title":"The Caries Microbiome: Implications for Reversing Dysbiosis","volume":"29","author":"Tanner","year":"2018","journal-title":"Adv. Dent. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1128\/IAI.00361-10","article-title":"Structural dissection and in vivo effectiveness of a peptide inhibitor of Porphyromonas gingivalis adherence to Streptococcus gordonii","volume":"79","author":"Daep","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1128\/IAI.72.3.1341-1348.2004","article-title":"Glyceraldehyde-3-phosphate dehydrogenase of Streptococcus oralis functions as a coadhesin for Porphyromonas gingivalis major fimbriae","volume":"72","author":"Maeda","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1111\/jcpe.12682","article-title":"Role of microbial biofilms in the maintenance of oral health and in the development of dental caries and periodontal diseases. Consensus report of group 1 of the Joint EFP\/ORCA workshop on the boundaries between caries and periodontal disease","volume":"44","author":"Sanz","year":"2017","journal-title":"J. Clin. Periodontol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/ismej.2012.174","article-title":"The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation","volume":"7","author":"Abusleme","year":"2013","journal-title":"ISME J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hong, B.Y., Furtado Araujo, M.V., Strausbaugh, L.D., Terzi, E., Ioannidou, E., and Diaz, P.I. (2015). Microbiome profiles in periodontitis in relation to host and disease characteristics. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0127077"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.foodcont.2010.02.003","article-title":"Antimicrobial herb and spice compounds in food","volume":"21","author":"Tajkarimi","year":"2010","journal-title":"Food Control"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"469","DOI":"10.5567\/pharmacologia.2013.469.480","article-title":"Perspective on plant products as antimicrobials agents: A review","volume":"4","author":"Pandey","year":"2013","journal-title":"Pharmacologia"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0140-6736(01)05701-4","article-title":"Effects of pretreatment with clopidogrel and aspirin followed by long-term therapy in patients undergoing percutaneous coronary intervention: The PCI-CURE study","volume":"358","author":"Mehta","year":"2001","journal-title":"Lancet"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hurtukov\u00e1, K., Fajstavrov\u00e1, K., Rimpelov\u00e1, S., Vokat\u00e1, B., Fajstavr, D., Kas\u00e1lkov\u00e1, N.S., Siegel, J., \u0160vor\u010d\u00edk, V., and Slepi\u010dka, P. (2021). Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles. Materials, 14.","DOI":"10.3390\/ma14144051"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"\u0160kubn\u00edk, J., Pavl\u00ed\u010dkov\u00e1, V., Ruml, T., and Rimpelov\u00e1, S. (2021). Current Perspectives on Taxanes: Focus on Their Bioactivity, Delivery and Combination Therapy. Plants, 10.","DOI":"10.3390\/plants10030569"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1038\/nbt1206-1504","article-title":"Prospects for plant-derived antibacterials","volume":"24","author":"Lewis","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"ref_28","unstructured":"Ody, P. (2017). The Complete Medicinal Herbal: A Practical Guide to the Healing Properties of Herbs, Simon and Schuster."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.ajps.2019.03.002","article-title":"A review on anti-bacterials to combat resistance: From ancient era of plants and metals to present and future perspectives of green nano technological combinations","volume":"15","author":"Ruddaraju","year":"2020","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.phymed.2008.12.018","article-title":"Synergy research: Approaching a new generation of phytopharmaceuticals","volume":"16","author":"Wagner","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_31","unstructured":"Joshi, R.K. (2017). Recent Advances in Drug Delivery Technology, IGI Global."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.jep.2011.06.032","article-title":"Evaluation of an ethnopharmacologically selected Bhutanese medicinal plants for their major classes of phytochemicals and biological activities","volume":"137","author":"Wangchuk","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.indcrop.2019.02.051","article-title":"Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review","volume":"132","author":"Knezevic","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1039\/D0QO01210F","article-title":"A unique high-diversity natural product collection as a reservoir of new therapeutic leads","volume":"8","author":"Ghirga","year":"2021","journal-title":"Org. Chem. Front."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Liu, L., Yu, J., Shen, X., Cao, X., Zhan, Q., Guo, Y., and Yu, F. (2020). Resveratrol enhances the antimicrobial effect of polymyxin B on Klebsiella pneumoniae and Escherichia coli isolates with polymyxin B resistance. BMC Microbiol., 20.","DOI":"10.1186\/s12866-020-01995-1"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.foodcont.2018.07.011","article-title":"Antibacterial activity of gallic acid against Shigella flexneri and its effect on biofilm formation by repressing mdoH gene expression","volume":"94","author":"Kang","year":"2018","journal-title":"Food Control"},{"key":"ref_37","first-page":"ZC01","article-title":"Anti-microbial efficacy of soursop leaf extract (Annona muricata) on oral pathogens: An in-vitro study","volume":"10","author":"Pai","year":"2016","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_38","first-page":"27","article-title":"Activity of bioactive garlic compounds on the oral microbiome: A literature review","volume":"68","author":"Hoglund","year":"2020","journal-title":"Gen. Dent."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"112620","DOI":"10.1016\/j.lwt.2021.112620","article-title":"Plant-based proteins and their multifaceted industrial applications","volume":"154","author":"Kumar","year":"2022","journal-title":"LWT"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Mitra, S., Anand, U., Sanyal, R., Jha, N.K., Behl, T., Mundhra, A., Ghosh, A., Kumar, M., Pro\u0107k\u00f3w, J., and Dey, A. (2022). Neoechinulins: Molecular, cellular, and functional attributes as promising therapeutics against cancer and other human diseases. Biomed. Pharmacother., 145.","DOI":"10.1016\/j.biopha.2021.112378"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sasi, M., Kumar, S., Kumar, M., Thapa, S., Prajapati, U., Tak, Y., Changan, S., Saurabh, V., Kumari, S., and Kumar, A. (2021). Garlic (Allium sativum L.) bioactives and its role in alleviating oral pathologies. Antioxidants, 10.","DOI":"10.3390\/antiox10111847"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Phitaktim, S., Chomnawang, M., Sirichaiwetchakoon, K., Dunkhunthod, B., Hobbs, G., and Eumkeb, G. (2016). Synergism and the mechanism of action of the combination of \u03b1-mangostin isolated from Garcinia mangostana L. and oxacillin against an oxacillin-resistant Staphylococcus saprophyticus. BMC Microbiol., 16.","DOI":"10.1186\/s12866-016-0814-4"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Farooqui, A., Khan, A., Borghetto, I., Kazmi, S.U., Rubino, S., and Paglietti, B. (2015). Synergistic antimicrobial activity of Camellia sinensis and Juglans regia against multidrug-resistant bacteria. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0118431"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/j.phytochem.2005.06.008","article-title":"Multidrug resistance reversal agent from Jatropha elliptica","volume":"66","author":"Marquez","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1039\/c2np20035j","article-title":"Plants as sources of new antimicrobials and resistance-modifying agents","volume":"29","author":"Abreu","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Mahmoud, R.Y., Trizna, E.Y., Sulaiman, R.K., Pavelyev, R.S., Gilfanov, I.R., Lisovskaya, S.A., Ostolopovskaya, O.V., Frolova, L.L., Kutchin, A.V., and Guseva, G.B. (2022). Increasing the Efficacy of Treatment of Staphylococcus aureus\u2013Candida albicans Mixed Infections with Myrtenol. Antibiotics, 11.","DOI":"10.3390\/antibiotics11121743"},{"key":"ref_47","first-page":"131","article-title":"Hops\u2014A millennium review","volume":"58","author":"Moir","year":"2000","journal-title":"J. Am. Soc. Brew. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijfoodmicro.2015.02.030","article-title":"Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria","volume":"202","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_49","first-page":"399","article-title":"Antifungal activities of hop bitter resins and related compounds","volume":"49","author":"Mizobuchi","year":"1985","journal-title":"Agric. Biol. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.indcrop.2019.04.001","article-title":"Phenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) Seeds","volume":"134","author":"Pinela","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1016\/j.ejca.2005.04.012","article-title":"Beer constituents as potential cancer chemopreventive agents","volume":"41","year":"2005","journal-title":"Eur. J. Cancer"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.freeradbiomed.2017.02.018","article-title":"Xanthohumol isolated from Humulus lupulus prevents thrombosis without increased bleeding risk by inhibiting platelet activation and mtDNA release","volume":"108","author":"Xin","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.phymed.2008.12.021","article-title":"In vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components (Humulus lupulus) addressing acne vulgaris","volume":"16","author":"Yamaguchi","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/ng.2478","article-title":"Emergence and global spread of epidemic healthcare-associated Clostridium difficile","volume":"45","author":"He","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.anifeedsci.2015.06.006","article-title":"Hops \u03b2-acids and zinc bacitracin affect the performance and intestinal microbiota of broilers challenged with Eimeria acervulina and Eimeria tenella","volume":"207","author":"Bortoluzzi","year":"2015","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1663\/0013-0001(2003)057[0118:IOSMAO]2.0.CO;2","article-title":"Inhibition of Streptococcus mutans and Other Oral streptococci by hop (Humulus lupulus L.) constituents","volume":"57","author":"Bhattacharya","year":"2003","journal-title":"Econ. Bot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"24","DOI":"10.22159\/ajpcr.2017.v10i2.15334","article-title":"Effect of anti-biofilm potential of different medicinal plants: Review","volume":"10","author":"Mani","year":"2017","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"103497","DOI":"10.1016\/j.jdent.2020.103497","article-title":"Current uses of chlorhexidine for management of oral disease: A narrative review","volume":"103","author":"Brookes","year":"2020","journal-title":"J. Dent."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"8326","DOI":"10.1038\/s41598-019-44822-y","article-title":"Development of antiseptic adaptation and cross-adapatation in selected oral pathogens in vitro","volume":"9","author":"Verspecht","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1007\/s00134-018-5171-3","article-title":"Effects of chlorhexidine gluconate oral care on hospital mortality: A hospital-wide, observational cohort study","volume":"44","author":"Deschepper","year":"2018","journal-title":"Intensive Care Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"302","DOI":"10.17221\/261\/2014-CJFS","article-title":"Inhibitory effects of fresh hops on Helicobacter pylori strains","volume":"33","author":"Strohalm","year":"2015","journal-title":"Czech J. Food Sci."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Stompor, M., and \u017barowska, B. (2016). Antimicrobial Activity of Xanthohumol and Its Selected Structural Analogues. Molecules, 21.","DOI":"10.3390\/molecules21050608"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Di Lodovico, S., Menghini, L., Ferrante, C., Recchia, E., Castro-Amorim, J., Gameiro, P., Cellini, L., and Bessa, L.J. (2020). Hop Extract: An Efficacious Antimicrobial and Anti-biofilm Agent against Multidrug-Resistant Staphylococci Strains and. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01852"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1172\/JCI102177","article-title":"Factors Influencing the Antibiotic Activity of Lupulon","volume":"28","author":"Chin","year":"1949","journal-title":"J. Clin. Investig."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jchromb.2018.07.018","article-title":"Xanthohumol C, a minor bioactive hop compound: Production, purification strategies and antimicrobial test","volume":"1095","author":"Roehrer","year":"2018","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1365-2672.1992.tb01843.x","article-title":"Factors affecting antibacterial activity of hop compounds and their derivatives","volume":"72","author":"Simpson","year":"1992","journal-title":"J. Appl. Bacteriol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nrmicro2381","article-title":"Oral multispecies biofilm development and the key role of cell-cell distance","volume":"8","author":"Kolenbrander","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.resmic.2017.12.005","article-title":"Antibiofilm activity of bioactive hop compounds humulone, lupulone and xanthohumol toward susceptible and resistant staphylococci","volume":"169","author":"Bogdanova","year":"2018","journal-title":"Res. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Mironova, A.V., Karimova, A.V., Bogachev, M.I., Kayumov, A.R., and Trizna, E.Y. (2023). Alterations in Antibiotic Susceptibility of Staphylococcus aureus and Klebsiella pneumoniae in Dual Species Biofilms. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24108475"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"16284","DOI":"10.1038\/s41598-019-52726-0","article-title":"Optimal environmental and culture conditions allow the in vitro coexistence of Pseudomonas aeruginosa and Staphylococcus aureus in stable biofilms","volume":"9","author":"Cendra","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Booth, S.C., and Rice, S.A. (2020). Influence of interspecies interactions on the spatial organization of dual species bacterial communities. Biofilm, 2.","DOI":"10.1016\/j.bioflm.2020.100035"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0031-9422(02)00446-6","article-title":"Bacterial resistance modifying agents from Lycopus europaeus","volume":"62","author":"Gibbons","year":"2003","journal-title":"Phytochemistry"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.phymed.2007.10.008","article-title":"Positive antibacterial co-action between hop (Humulus lupulus) constituents and selected antibiotics","volume":"15","author":"Natarajan","year":"2008","journal-title":"Phytomedicine"},{"key":"ref_74","unstructured":"Gagos, M. (2017). Process for Preparation of Xanthohumol. (US9556097B2), Patent."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.jasms.2003.09.014","article-title":"Direct characterization of bitter acids in a crude hop extract by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry","volume":"15","author":"Zhang","year":"2004","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"9386","DOI":"10.1021\/jf201801q","article-title":"Characterization of phenolic components in polar extracts of Eucalyptus globulus Labill. bark by high-performance liquid chromatography\u2013mass spectrometry","volume":"59","author":"Santos","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1590\/S0102-695X2010005000051","article-title":"Bitter acids from hydroethanolic extracts of Humulus lupulus L., Cannabaceae, used as anxiolytic","volume":"20","author":"Negri","year":"2010","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_78","first-page":"289","article-title":"Humulus lupulus\u2013a story that begs to be told","volume":"120","author":"Almaguer","year":"2014","journal-title":"A review. J. Inst. Brew."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/ja.2014.143","article-title":"Inhibition of biofilm formation in Bacillus subtilis by new halogenated furanones","volume":"68","author":"Kayumov","year":"2015","journal-title":"J. Antibiot."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"102","DOI":"10.32607\/20758251-2015-7-2-102-107","article-title":"Thio Derivatives of 2(5H)-Furanone As Inhibitors against Bacillus subtilis Biofilms","volume":"7","author":"Trizna","year":"2015","journal-title":"Acta Naturae"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1469-0691.2011.03703.x","article-title":"EUCAST expert rules in antimicrobial susceptibility testing","volume":"19","author":"Leclercq","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Sharafutdinov, I.S., Trizna, E.Y., Baidamshina, D.R., Ryzhikova, M.N., Sibgatullina, R.R., Khabibrakhmanova, A.M., Latypova, L.Z., Kurbangalieva, A.R., Rozhina, E.V., and Klinger-Strobel, M. (2017). Antimicrobial Effects of Sulfonyl Derivative of 2(5H)-Furanone against Planktonic and Biofilm Associated Methicillin-Resistant and -Susceptible Staphylococcus aureus. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02246"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Sulaiman, R., Trizna, E., Kolesnikova, A., Khabibrakhmanova, A., Kurbangalieva, A., Bogachev, M., and Kayumov, A. (2022). Antimicrobial and Biofilm-Preventing Activity of l-Borneol Possessing 2 (5 H)-Furanone Derivative F131 against S. aureus\u2014C. albicans Mixed Cultures. Pathogens, 12.","DOI":"10.3390\/pathogens12010026"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jac\/dkg301","article-title":"Synergy, antagonism, and what the chequerboard puts between them","volume":"52","author":"Odds","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Bogachev, M.I., Volkov, V.Y., Markelov, O.A., Trizna, E.Y., Baydamshina, D.R., Melnikov, V., Murtazina, R.R., Zelenikhin, P.V., Sharafutdinov, I.S., and Kayumov, A.R. (2018). Fast and simple tool for the quantification of biofilm-embedded cells sub-populations from fluorescent microscopic images. 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