{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:36:06Z","timestamp":1762900566786,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Ministry of Education, Research, Development and Youth of the Slovak Republic","award":["VEGA 1\/0428\/21","VEGA 1\/0042\/24"],"award-info":[{"award-number":["VEGA 1\/0428\/21","VEGA 1\/0042\/24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Adenosine is a multifunctional nucleoside with several roles across various levels in organisms. Beyond its intracellular involvement in cellular metabolism, extracellular adenosine potently influences both physiological and pathological processes. In relation to its blood level, adenosine impacts the cardiovascular system, such as heart beat rate and vasodilation. To exploit the adenosine levels in the blood, we employed the liquid chromatography method coupled with mass spectrometry (LC\u2013MS). Immediately after collection, a blood sample mixed with acetonitrile solution that is either enriched with 13C-labeled adenosine or a newly generated mixture is transferred into the tubes containing the defined amount of 13C-labeled adenosine. The 13C-enriched isotopic adenosine is used as an internal standard, allowing for more accurate quantification of adenosine. This novel protocol for LC\u2013MS-based estimation of adenosine delivers a rapid, highly sensitive, and reproducible means for quantitative estimation of total adenosine in blood. The method also allows for quantification of a few catabolites of adenosine, i.e., inosine, hypoxanthine, and xanthine. Our current setup did not allow for the detection or quantifying of uric acid, which is the final product of adenosine catabolism. This advancement provides an analytical tool that has the potential to enhance our understanding of adenosine\u2019s systemic impact and pave the way for further investigations into its intricate regulatory mechanisms.<\/jats:p>","DOI":"10.3390\/ph17030345","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T04:19:02Z","timestamp":1709785142000},"page":"345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Application of Liquid Chromatography Coupled to Mass Spectrometry for Direct Estimation of the Total Levels of Adenosine and Its Catabolites in Human Blood"],"prefix":"10.3390","volume":"17","author":[{"given":"Jakub","family":"\u0160ofranko","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7075-9480","authenticated-orcid":false,"given":"Peter","family":"Mitro","sequence":"additional","affiliation":[{"name":"Cardiology Clinic VUSCH, \u0160afarik University, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zora","family":"Laz\u00farov\u00e1","sequence":"additional","affiliation":[{"name":"Cardiology Clinic VUSCH, \u0160afarik University, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin Jozef","family":"P\u00e9\u010d","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Bolek","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"},{"name":"Department of Cardiology, Teaching Hospital in Tren\u010d\u00edn, 911 71 Tren\u010d\u00edn, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"P\u00e9\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00fa\u0161","family":"Doh\u00e1l","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, 832 32 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matej","family":"Samo\u0161","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"},{"name":"Department of Cardiology, Teaching Hospital in Tren\u010d\u00edn, 911 71 Tren\u010d\u00edn, Slovakia"},{"name":"Division of Invasive and Interventional Cardiology, Department of Acute and Interventional Cardiology, Mid-Slovakian Institute of Heart and Vessel Diseases (S\u00daSCCH, a.s.) in Bansk\u00e1 Bystrica, 974 01 Bansk\u00e1 Bystrica, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2952-0398","authenticated-orcid":false,"given":"Radovan","family":"Mur\u00edn","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.ccell.2019.10.007","article-title":"Adenosine Metabolism: Emerging Concepts for Cancer Therapy","volume":"36","author":"Boison","year":"2019","journal-title":"Cancer Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1146\/annurev.neuro.24.1.31","article-title":"The Role and Regulation of Adenosine in the Central Nervous System","volume":"24","author":"Dunwiddie","year":"2001","journal-title":"Annu. 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