{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T08:39:09Z","timestamp":1784018349428,"version":"3.55.0"},"reference-count":20,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shandong Province Chinese Medicine Science and Technology Project Youth Project","award":["Q-2023097"],"award-info":[{"award-number":["Q-2023097"]}]},{"name":"Shandong Province Chinese Medicine Science and Technology Project Youth Project","award":["No. zyyzdxk-2023124"],"award-info":[{"award-number":["No. zyyzdxk-2023124"]}]},{"name":"Shandong Province Chinese Medicine Science and Technology Project Youth Project","award":["2023058"],"award-info":[{"award-number":["2023058"]}]},{"name":"Shandong Province Chinese Medicine Science and Technology Project Youth Project","award":["2023060"],"award-info":[{"award-number":["2023060"]}]},{"name":"Marine Traditional Chinese Medicine","award":["Q-2023097"],"award-info":[{"award-number":["Q-2023097"]}]},{"name":"Marine Traditional Chinese Medicine","award":["No. zyyzdxk-2023124"],"award-info":[{"award-number":["No. zyyzdxk-2023124"]}]},{"name":"Marine Traditional Chinese Medicine","award":["2023058"],"award-info":[{"award-number":["2023058"]}]},{"name":"Marine Traditional Chinese Medicine","award":["2023060"],"award-info":[{"award-number":["2023060"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["Q-2023097"],"award-info":[{"award-number":["Q-2023097"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["No. zyyzdxk-2023124"],"award-info":[{"award-number":["No. zyyzdxk-2023124"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["2023058"],"award-info":[{"award-number":["2023058"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["2023060"],"award-info":[{"award-number":["2023060"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["Q-2023097"],"award-info":[{"award-number":["Q-2023097"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["No. zyyzdxk-2023124"],"award-info":[{"award-number":["No. zyyzdxk-2023124"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["2023058"],"award-info":[{"award-number":["2023058"]}]},{"name":"Shandong University of Traditional Chinese Medicine","award":["2023060"],"award-info":[{"award-number":["2023060"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Chalcone (1,3-diaryl-2-propen-1-one) is an \u03b1, \u03b2-unsaturated ketone that serves as an active constituent or precursor of numerous natural substances, exhibiting a broad spectrum of pharmacological effects. In this study, the classical Claisen\u2013Schmidt condensation method was used to synthesize the chalcone derivative 2\u2032,4\u2032-dimethoxychalcone (DTC) and evaluate its pharmacological activity. By upregulating the expression of the epithelial cell marker E-cadherin and downregulating the expression of the mesenchymal cell marker vimentin, DTC was found to inhibit transforming growth factor-\u03b21 (TGF-\u03b21)-induced epithelial\u2013mesenchymal transition (EMT) process in A549 cells, maintaining the cells\u2019 epithelial-like morphology and reducing the ability of the cells to migrate. Additionally, DTC demonstrated the ability to decrease the expression levels of nitric oxide (NO), tumor necrosis factor (TNF-\u03b1), interleukin-6 (IL-6), and interleukin-1\u03b2 (IL-1\u03b2) in RAW264.7 cells, suggesting a possible anti-inflammatory effect. Furthermore, DTC was found to exhibit bacteriostatic activity against Staphylococcus aureus (S. aureus), Proteus vulgaris (P. vulgaris), methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans (C. albicans), indicating that this chemical may possess broad-spectrum antibacterial activity.<\/jats:p>","DOI":"10.3390\/ph17050653","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T06:53:49Z","timestamp":1715928829000},"page":"653","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Testing of Anti-EMT, Anti-Inflammatory and Antibacterial Activities of 2\u2032,4\u2032-Dimethoxychalcone"],"prefix":"10.3390","volume":"17","author":[{"given":"Peiling","family":"Zhao","sequence":"first","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengzhen","family":"Xu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Gong","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaihui","family":"Lu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Ruan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shandong University of traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixing","family":"Guan","sequence":"additional","affiliation":[{"name":"Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Shandong Provincial Key Laboratory of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jayachandran, J., Srinivasan, H., and Mani, K.P. (2021). Molecular mechanism involved in epithelial to mesenchymal transition. Arch. Biochem. Biophys., 710.","DOI":"10.1016\/j.abb.2021.108984"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chen, H.-Y., Chiang, Y.-F., Huang, J.-S., Huang, T.-C., Shih, Y.-H., Wang, K.-L., Ali, M., Hong, Y.-H., Shieh, T.-M., and Hsia, S.-M. (2021). Isoliquiritigenin Reverses Epithelial-Mesenchymal Transition Through Modulation of the TGF-\u03b2\/Smad Signaling Pathway in Endometrial Cancer. Cancers, 13.","DOI":"10.3390\/cancers13061236"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"114207","DOI":"10.1016\/j.physbeh.2023.114207","article-title":"Butein ameliorates chronic stress induced atherosclerosis via targeting anti-inflammatory, anti-fibrotic and BDNF pathways","volume":"267","author":"Rehman","year":"2023","journal-title":"Physiol. Behav."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e2100749","DOI":"10.1002\/advs.202100749","article-title":"Plant Natural Flavonoids Against Multidrug Resistant Pathogens","volume":"8","author":"Song","year":"2021","journal-title":"Adv. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ouyang, Y., Li, J., Chen, X., Fu, X., Sun, S., and Wu, Q. (2021). Chalcone Derivatives: Role in Anticancer Therapy. Biomolecules, 11.","DOI":"10.3390\/biom11060894"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"881","DOI":"10.2174\/1573406415666190724145158","article-title":"Methoxychalcones: Effect of Methoxyl Group on the Antifungal, Antibacterial and Antiproliferative Activities","volume":"16","author":"Marques","year":"2020","journal-title":"Med. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.31557\/APJCP.2021.22.8.2409","article-title":"Molecular Docking Compounds of Cinnamaldehyde Derivatives as Anticancer Agents","volume":"22","author":"Warsito","year":"2021","journal-title":"Asian Pac. J. Cancer Prev. APJCP"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"378","DOI":"10.3389\/fphar.2020.00378","article-title":"Isoliquiritigenin Derivative Regulates miR-374a\/BAX Axis to Suppress Triple-Negative Breast Cancer Tumorigenesis and Development","volume":"11","author":"Peng","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7762","DOI":"10.1021\/acs.chemrev.7b00020","article-title":"Chalcone: A Privileged Structure in Medicinal Chemistry","volume":"117","author":"Zhuang","year":"2017","journal-title":"Chem. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1007\/s12272-015-0595-6","article-title":"Discovery of 2\u2032,4\u2032-dimethoxychalcone as a Hsp90 inhibitor and its effect on iressa-resistant non-small cell lung cancer (NSCLC)","volume":"38","author":"Seo","year":"2015","journal-title":"Arch. Pharmacal Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"906927","DOI":"10.3389\/fphar.2022.906927","article-title":"Anti-Inflammatory Effects and Mechanisms of Dandelion in RAW264.7 Macrophages and Zebrafish Larvae","volume":"13","author":"Li","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41580-020-0237-9","article-title":"Guidelines and definitions for research on epithelial-mesenchymal transition","volume":"21","author":"Yang","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1016\/j.bbadis.2015.06.008","article-title":"Crosstalk between calpain activation and TGF-\u03b21 augments collagen-I synthesis in pulmonary fibrosis","volume":"1852","author":"Li","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00441-021-03574-w","article-title":"Epithelial-mesenchymal transition process during embryo implantation","volume":"388","author":"Oghbaei","year":"2022","journal-title":"Cell Tissue Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Marconi, G.D., Fonticoli, L., Rajan, T.S., Pierdomenico, S.D., Trubiani, O., Pizzicannella, J., and Diomede, F. (2021). Epithelial-Mesenchymal Transition (EMT): The Type-2 EMT in Wound Healing, Tissue Regeneration and Organ Fibrosis. Cells, 10.","DOI":"10.3390\/cells10071587"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e108647","DOI":"10.15252\/embj.2021108647","article-title":"Dynamic EMT: A multi-tool for tumor progression","volume":"40","author":"Brabletz","year":"2021","journal-title":"EMBO J."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cao, Y., Chen, J., Ren, G., Zhang, Y., Tan, X., and Yang, L. (2019). Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a\/Autophagy Signaling Pathway. Nutrients, 11.","DOI":"10.3390\/nu11112794"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/s41429-020-0280-y","article-title":"Ferrocenyl chalcone derivatives as possible antimicrobial agents","volume":"73","author":"Henry","year":"2020","journal-title":"J. Antibiot."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Koudokpon, H., Armstrong, N., Dougnon, T.V., Fah, L., Hounsa, E., Bankol\u00e9, H.S., Loko, F., Chabri\u00e8re, E., and Rolain, J.M. (2018). Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria. BioMed Res. Int., 2018.","DOI":"10.1155\/2018\/1453173"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s12275-024-00103-6","article-title":"Furan-based Chalcone Annihilates the Multi-Drug-Resistant Pseudomonas aeruginosa and Protects Zebra Fish Against its Infection","volume":"62","author":"Nayak","year":"2024","journal-title":"J. Microbiol."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/5\/653\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:44:00Z","timestamp":1760107440000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/5\/653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,17]]},"references-count":20,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["ph17050653"],"URL":"https:\/\/doi.org\/10.3390\/ph17050653","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,17]]}}}