{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T03:36:01Z","timestamp":1773977761948,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009392","name":"Prince Sattam bin Abdulaziz University","doi-asserted-by":"publisher","award":["PSAU\/2024\/R\/1445"],"award-info":[{"award-number":["PSAU\/2024\/R\/1445"]}],"id":[{"id":"10.13039\/100009392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Bacterial resistance and virulence are challenges in treating bacterial infections, especially in Klebsiella pneumoniae. Plants of the Launaea Cass. genus are used traditionally to address a variety of diseases, including infections, but the potential bioactive compounds are unknown. Our goals were to verify the potential contribution of two major polyacetylene glycosides isolated from our previous study, (3S,6E,12E)-6,12-tetradecadiene-8,10-diyne-1-ol 3-O-\u03b2-D-glucopyranoside (1) and bidensyneoside A (syn. gymnasterkoreaside A) [(3R,8E)-3-hydroxy-8-decene-4,6-diyn-1-yl \u03b2-D-glucopyranoside] (2), to the anti-infective properties of Launaea capitata and to develop a dependable HPLC method for their quantification; Methods: On a panel of K. pneumoniae clinical isolates, the antibacterial action of 1, 2, and the methanol extract of the whole L. capitata plant were evaluated by broth microdilution assay, while their antibiofilm action was evaluated by the crystal violet assay. qRT-PCR investigated luxS, mrkA, wzm, and wbbm genes that encode biofilm formation and quorum sensing (QS). The antibacterial activity of 1 was revealed by employing mice infection. Chromatographic separation was conducted using isocratic elution on a Hypersil BDS C18 column using a photodiode array (PDA) detector; Results: Compound 1 showed antibacterial activity with MIC values of 16\u2013128 \u00b5g\/mL. It remarkably reduced strong and moderate biofilm-forming bacterial isolates from 84.21% to 42.1% compared with the extract (68.42%) and 2 (78.95%). Compound 1 also downregulated the QS genes, luxS, mrkA, wzm, and wbbm, and exhibited in vivo antibacterial action through the enhancement of the histological construction of the liver and spleen, decreased TNF-\u03b1 immunoreaction, bacterial burden, and the inflammatory mediators IL-1\u03b2 and IL-6. A successful HPLC-PDA approach was developed to separate the binary mixture of 1 and 2 in less than 10 min with high sensitivity, with detection limits down to 0.518 and 0.095 \u00b5g\/mL for 1 and 2, respectively; Conclusions: Compound 1 exhibited remarkable antibacterial and antibiofilm properties and may contribute to the anti-infectious traditional uses of L. capitata, meriting further clinical studies and serving as a reliable quality control biomarker for the plant.<\/jats:p>","DOI":"10.3390\/ph17091214","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:14:07Z","timestamp":1726460047000},"page":"1214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["HPLC-PDA Analysis of Polyacetylene Glucosides from Launaea capitata and Their Antibacterial and Antibiofilm Properties against Klebsiella pneumoniae"],"prefix":"10.3390","volume":"17","author":[{"given":"Tariq M.","family":"Aljarba","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0823-4015","authenticated-orcid":false,"given":"Fatma M.","family":"Abdel Bar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia"},{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmaa E.","family":"Sherif","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia"},{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8287-1026","authenticated-orcid":false,"given":"Engy","family":"Elekhnawy","sequence":"additional","affiliation":[{"name":"Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-5065","authenticated-orcid":false,"given":"Galal","family":"Magdy","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh 33511, Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura National University, Gamasa 7731168, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6517-9409","authenticated-orcid":false,"given":"Reham M.","family":"Samra","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,15]]},"reference":[{"key":"ref_1","first-page":"277","article-title":"The antibiotic resistance crisis: Part 1: Causes and threats","volume":"40","author":"Ventola","year":"2015","journal-title":"Pharm. Ther."},{"key":"ref_2","first-page":"3","article-title":"Tackling Threats and Future Problems of Multidrug-Resistant Bacteria","volume":"398","author":"Medina","year":"2016","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kot, B., Piechota, M., Szweda, P., Mitrus, J., Wicha, J., Gru\u017cewska, A., and Witeska, M. (2023). Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-31086-w"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hafiz, T.A., Alanazi, S., Alghamdi, S.S., Mubaraki, M.A., Aljabr, W., Madkhali, N., Alharbi, S.R., Binkhamis, K., and Alotaibi, F. (2023). Klebsiella pneumoniae bacteraemia epidemiology: Resistance profiles and clinical outcome of King Fahad Medical City isolates, Riyadh, Saudi Arabia. BMC Infect. Dis., 23.","DOI":"10.1186\/s12879-023-08563-8"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.14202\/vetworld.2022.2172-2179","article-title":"A review: Virulence factors of Klebsiella pneumonia as emerging infection on the food chain","volume":"15","author":"Riwu","year":"2022","journal-title":"Vet. World"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Liu, X., Yao, H., Zhao, X., and Ge, C. (2023). Biofilm Formation and Control of Foodborne Pathogenic Bacteria. Molecules, 28.","DOI":"10.3390\/molecules28062432"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ram\u00edrez-Larrota, J.S., and Eckhard, U. (2022). An introduction to bacterial biofilms and their proteases, and their roles in host infection and immune evasion. Biomolecules, 12.","DOI":"10.3390\/biom12020306"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Latka, A., and Drulis-Kawa, Z. (2020). Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-77198-5"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Jiang, Q., Chen, J., Yang, C., Yin, Y., and Yao, K. (2019). Quorum sensing: A prospective therapeutic target for bacterial diseases. BioMed Res. Int., 2019.","DOI":"10.1155\/2019\/2015978"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zhao, X., Yu, Z., and Ding, T. (2020). Quorum-sensing regulation of antimicrobial resistance in bacteria. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030425"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wang, L., Zhang, Y., Liu, Y., Xu, M., Yao, Z., Zhang, X., Sun, Y., Zhou, T., and Shen, M. (2022). Effects of chlorogenic acid on antimicrobial, antivirulence, and anti-quorum sensing of carbapenem-resistant Klebsiella pneumoniae. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.997310"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Li, Y., and Ni, M. (2023). Regulation of biofilm formation in Klebsiella pneumoniae. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1238482"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Uru\u00e9n, C., Chopo-Escuin, G., Tommassen, J., Mainar-Jaime, R.C., and Arenas, J. (2020). Biofilms as Promoters of Bacterial Antibiotic Resistance and Tolerance. Antibiotics, 10.","DOI":"10.3390\/antibiotics10010003"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sharma, S., Mohler, J., Mahajan, S.D., Schwartz, S.A., Bruggemann, L., and Aalinkeel, R. (2023). Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061614"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Oliveira, R., Castro, J., Silva, S., Oliveira, H., Saavedra, M.J., Azevedo, N.F., and Almeida, C. (2022). Exploring the antibiotic resistance profile of clinical Klebsiella pneumoniae isolates in Portugal. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111613"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mancuso, G., Midiri, A., Gerace, E., and Biondo, C. (2021). Bacterial antibiotic resistance: The most critical pathogens. Pathogens, 10.","DOI":"10.3390\/pathogens10101310"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e14985","DOI":"10.1016\/j.heliyon.2023.e14985","article-title":"Antimicrobial activity in Asterceae: The selected genera characterization and against multidrug resistance bacteria","volume":"9","author":"Gou","year":"2023","journal-title":"Heliyon"},{"key":"ref_18","first-page":"23","article-title":"Plant flora in the rangeland of western Saudi Arabia","volume":"44","author":"Daur","year":"2012","journal-title":"Pak. J. Bot."},{"key":"ref_19","first-page":"399","article-title":"Isolation of phytoconstituents and evaluation of anticancer and Antioxidant potential of Launaea mucronata (Forssk.) Muschl. subsp","volume":"30","author":"Elsharkawy","year":"2017","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_20","first-page":"1","article-title":"Revision of Launaea Cass. (Compositae, Lactuceae, Sonchinae)","volume":"17","author":"Kilian","year":"1997","journal-title":"Englera"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Al-Fatimi, M. (2021). Wild edible plants traditionally collected and used in southern Yemen. J. Ethnobiol. Ethnomed., 17.","DOI":"10.1186\/s13002-021-00475-8"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.4314\/tjpr.v19i10.17","article-title":"Anti-proliferative activity of leaves of Launaea capitata Asteraceae: Phytochemical, cytotoxicity and in silico studies","volume":"19","author":"Khalil","year":"2020","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_23","first-page":"67","article-title":"Phytochemical and biological studies on Launaea Cass. genus (Asteraceae) from Algerian Sahara","volume":"11","author":"Cheriti","year":"2012","journal-title":"Curr. Top. Phytochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"104548","DOI":"10.1016\/j.fitote.2020.104548","article-title":"Three new polyacetylene glycosides (PAGs) from the aerial part of Launaea capitata (Asteraceae) with anti-biofilm activity against Staphylococcus aureus","volume":"143","author":"Emad","year":"2020","journal-title":"Fitoterapia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.sajb.2024.07.043","article-title":"Unveiling the role of macranthoin G in the traditional anti-infective properties of Launaea nudicaulis","volume":"172","author":"Elekhnawy","year":"2024","journal-title":"S. Afr. J. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Abdel Bar, F.M., Sherif, A.E., and ElNaggar, M.H. (2023). Galactolipids from Launaea capitata (Spreng.) Dandy with In Vitro Anti-Inflammatory and Neuroprotective Activities. Separations, 10.","DOI":"10.3390\/separations10020083"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Abdel Bar, F.M., Mira, A., Foudah, A.I., Alossaimi, M.A., Alkanhal, S.F., Aldaej, A.M., and ElNaggar, M.H. (2023). In Vitro and In Silico Investigation of Polyacetylenes from Launaea capitata (Spreng.) Dandy as Potential COX-2, 5-LOX, and BchE Inhibitors. Molecules, 28.","DOI":"10.3390\/molecules28083526"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8830","DOI":"10.1021\/jf070735o","article-title":"Quantification of two polyacetylenes in Radix Ginseng and roots of related Panax species using a gas chromatography-mass spectrometric method","volume":"55","author":"Liu","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1590\/S0102-695X2012005000051","article-title":"Identification and quantification of sesquiterpenes and polyacetylenes in Atractylodes lancea from various geographical origins using GC-MS analysis","volume":"22","author":"Ouyang","year":"2012","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.jpba.2006.01.057","article-title":"Bioactive polyacetylenes in food plants of the Apiaceae family: Occurrence, bioactivity and analysis","volume":"41","author":"Christensen","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Waksmundzka-Hajnos, M., and Sherma, J. (2010). HPLC Analysis of Polyacetylenes. High Performance Liquid Chromatography in Phytochemical Analysis, CRC Press. [1st ed.].","DOI":"10.1201\/b10320"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/pca.2494","article-title":"HPLC\/UV Analysis of Polyacetylenes, Phenylpropanoid and Pyrrolidine Alkaloids in Medicinally Used Codonopsis Species","volume":"25","author":"He","year":"2014","journal-title":"Phytochem. Anal."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.chroma.2006.11.038","article-title":"High-performance liquid chromatography analysis of polyacetylenes and polyenes in Echinacea pallida by using a monolithic reversed-phase silica column","volume":"1149","author":"Pellati","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113288","DOI":"10.1016\/j.phytochem.2022.113288","article-title":"Polyacetylenes in herbal medicine: A comprehensive review of its occurrence, pharmacology, toxicology, and pharmacokinetics (2014\u20132021)","volume":"201","author":"Xie","year":"2022","journal-title":"Phytochemistry"},{"key":"ref_35","unstructured":"ICH (2005). Validation of analytical procedures: Text and methodology. Q2 (R1), International Conference on Harmonization."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Martin, R.M., and Bachman, M.A. (2018). Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00004"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5309350","DOI":"10.1155\/2022\/5309350","article-title":"Prevalence of multidrug-resistant Klebsiella pneumoniae clinical isolates in Nepal","volume":"2022","author":"Odari","year":"2022","journal-title":"J. Trop. Med."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Awoke, T., Teka, B., Seman, A., Sebre, S., Yeshitela, B., Aseffa, A., Mihret, A., and Abebe, T. (2021). High prevalence of multidrug-resistant Klebsiella pneumoniae in a tertiary care hospital in Ethiopia. Antibiotics, 10.","DOI":"10.3390\/antibiotics10081007"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Okafor, J.U., and Nwodo, U.U. (2023). Molecular Characterization of Antibiotic Resistance Determinants in Klebsiella pneumoniae Isolates Recovered from Hospital Effluents in the Eastern Cape Province, South Africa. Antibiotics, 12.","DOI":"10.3390\/antibiotics12071139"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Samtiya, M., Aluko, R.E., Dhewa, T., and Moreno-Rojas, J.M. (2021). Potential health benefits of plant food-derived bioactive components: An overview. Foods, 10.","DOI":"10.3390\/foods10040839"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in vitro evaluating antimicrobial activity: A review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J. Pharm. Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jep.2015.10.032","article-title":"Three new anti-HBV active constituents from the traditional Chinese herb of Yin-Chen (Artemisia scoparia)","volume":"176","author":"Geng","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1021\/acs.jnatprod.7b00648","article-title":"Phenylpropenoids from Bupleurum fruticosum as anti-human rhinovirus species A selective capsid binders","volume":"80","author":"Fois","year":"2017","journal-title":"J. Nat. Prod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"7395","DOI":"10.3390\/molecules15107395","article-title":"Antibacterial activity of phytochemicals isolated from Atractylodes japonica against methicillin-resistant Staphylococcus aureus","volume":"15","author":"Jeong","year":"2010","journal-title":"Molecules"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1248\/cpb.37.193","article-title":"A New Polyacetylene Compound from Atractylodes rhizome","volume":"37","author":"Kano","year":"1989","journal-title":"Chem. Pharm. Bull."},{"key":"ref_46","first-page":"201","article-title":"Inhibitory effects of the rhizome extract of Atractylodes japonica on the proliferation of human tumor cell lines","volume":"36","author":"Lee","year":"2005","journal-title":"Korean J. Pharmacog."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1248\/cpb.22.1943","article-title":"Letter: Diacetyl-atractylodiol, a new acetylenic compound from Atractylodes japonica Koidzumi","volume":"22","author":"Yosioka","year":"1974","journal-title":"Chem. Pharm. Bull."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"El-Zamkan, M.A., and Mohamed, H.M. (2021). Antimicrobial resistance, virulence genes and biofilm formation in Enterococcus species isolated from milk of sheep and goat with subclinical mastitis. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0259584"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Alherz, F.A., Negm, W.A., Elekhnawy, E., El-Masry, T.A., Haggag, E.M., Alqahtani, M.J., and Hussein, I.A. (2022). Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates. J. Fungi, 8.","DOI":"10.3390\/jof8101005"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Guerra, M.E.S., Destro, G., Vieira, B., Lima, A.S., Ferraz, L.F.C., Hakansson, A.P., Darrieux, M., and Converso, T.R. (2022). Klebsiella pneumoniae biofilms and their role in disease pathogenesis. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.877995"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.15190\/d.2019.13","article-title":"Communication is the key: Biofilms, quorum sensing, formation and prevention","volume":"7","author":"Preda","year":"2019","journal-title":"Discoveries"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7204","DOI":"10.18632\/oncotarget.23208","article-title":"Inflammatory responses and inflammation-associated diseases in organs","volume":"9","author":"Chen","year":"2018","journal-title":"Oncotarget"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1100\/2011\/212680","article-title":"Role of the inflammasome, IL-1\u03b2, and IL-18 in bacterial infections","volume":"11","author":"Sahoo","year":"2011","journal-title":"Sci. World J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e5127","DOI":"10.1097\/MD.0000000000005127","article-title":"Serum interleukin-6 in the diagnosis of bacterial infection in cirrhotic patients: A meta-analysis","volume":"95","author":"Wu","year":"2016","journal-title":"Medicine"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1476-9255-7-42","article-title":"The inflammatory cytokine tumor necrosis factor modulates the expression of Salmonella typhimurium effector proteins","volume":"7","author":"Ma","year":"2010","journal-title":"J. Inflamm."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Kany, S., Vollrath, J.T., and Relja, B. (2019). Cytokines in inflammatory disease. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20236008"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.lfs.2017.07.030","article-title":"SIRT3 inhibits Ang II-induced transdifferentiation of cardiac fibroblasts through \u03b2-catenin\/PPAR-\u03b3 signaling","volume":"186","author":"Guo","year":"2017","journal-title":"Life Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1021\/np500605v","article-title":"Polyyne hybrid compounds from Notopterygium incisum with peroxisome proliferator-activated receptor gamma agonistic effects","volume":"77","author":"Liu","year":"2014","journal-title":"J. Nat. Prod."},{"key":"ref_59","first-page":"489","article-title":"A new polyacetylene and a new isonicotinic acid glucoside from Bidens parviflora Willd","volume":"12","author":"Li","year":"2020","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1080\/10520295.2020.1742932","article-title":"Panaxynol from Saposhnikovia diviaricata exhibits a hepatoprotective effect against lipopolysaccharide+ D-Gal N induced acute liver injury by inhibiting Nf-\u03baB\/I\u03baB-\u03b1 and activating Nrf2\/HO-1 signaling pathways","volume":"95","author":"Sun","year":"2020","journal-title":"Biotech. Histochem."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Osman, E.A., El-Amin, N., Adrees, E.A., Al-Hassan, L., and Mukhtar, M. (2020). Comparing conventional, biochemical and genotypic methods for accurate identification of Klebsiella pneumoniae in Sudan. Access Microbiol., 2.","DOI":"10.1099\/acmi.0.000096"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Alandiyjany, M.N., Abdelaziz, A.S., Abdelfattah-Hassan, A., Hegazy, W.A., Hassan, A.A., Elazab, S.T., Mohamed, E.A., El-Shetry, E.S., Saleh, A.A., and ElSawy, N.A. (2022). Novel in vivo assessment of antimicrobial efficacy of ciprofloxacin loaded mesoporous silica nanoparticles against Salmonella typhimurium infection. Pharmaceuticals, 15.","DOI":"10.3390\/ph15030357"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Attallah, N.G., Al-Fakhrany, O.M., Elekhnawy, E., Hussein, I.A., Shaldam, M.A., Altwaijry, N., Alqahtani, M.J., and Negm, W.A. (2022). Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches. Antibiotics, 11.","DOI":"10.3390\/antibiotics11080993"},{"key":"ref_64","first-page":"67","article-title":"Quorum sensing inhibition, relevance to periodontics","volume":"7","author":"Yada","year":"2015","journal-title":"J. Int. Oral Health"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/9\/1214\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:56:51Z","timestamp":1760111811000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/9\/1214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,15]]},"references-count":64,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ph17091214"],"URL":"https:\/\/doi.org\/10.3390\/ph17091214","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,15]]}}}