{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:08:49Z","timestamp":1792789729487,"version":"4.5.0"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002848","name":"FONDECYT","doi-asserted-by":"publisher","award":["1161092"],"award-info":[{"award-number":["1161092"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"FONDECYT","doi-asserted-by":"publisher","award":["1240129"],"award-info":[{"award-number":["1240129"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"FONDECYT","doi-asserted-by":"publisher","award":["11200057"],"award-info":[{"award-number":["11200057"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"FONDECYT","doi-asserted-by":"publisher","award":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"],"award-info":[{"award-number":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PRONII-CONACYT (National Research Incentive Program of the National Council for Science and Technology) in Paraguay","award":["1161092"],"award-info":[{"award-number":["1161092"]}]},{"name":"PRONII-CONACYT (National Research Incentive Program of the National Council for Science and Technology) in Paraguay","award":["1240129"],"award-info":[{"award-number":["1240129"]}]},{"name":"PRONII-CONACYT (National Research Incentive Program of the National Council for Science and Technology) in Paraguay","award":["11200057"],"award-info":[{"award-number":["11200057"]}]},{"name":"PRONII-CONACYT (National Research Incentive Program of the National Council for Science and Technology) in Paraguay","award":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"],"award-info":[{"award-number":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"]}]},{"name":"Education, Investigation, and Biotechnology applied to Health","award":["1161092"],"award-info":[{"award-number":["1161092"]}]},{"name":"Education, Investigation, and Biotechnology applied to Health","award":["1240129"],"award-info":[{"award-number":["1240129"]}]},{"name":"Education, Investigation, and Biotechnology applied to Health","award":["11200057"],"award-info":[{"award-number":["11200057"]}]},{"name":"Education, Investigation, and Biotechnology applied to Health","award":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"],"award-info":[{"award-number":["FOCEM\/MERCOSUR\/COF\/Number 03\/11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: In this study, the efficacy of benzo[h]chromene derivatives as antiprotozoal and antimycobacterial agents was explored. Methods: A total of twenty compounds, including benzo[h]chromene alkyl diesters and benzo[h]chromene-triazole derivatives, were synthesized and tested against Trypanosoma cruzi, Leishmania braziliensis, L. infantum, and strains of Mycobacterium abscessus and Mycobacterium intracellulare LIID-01. Notably, compounds 1a, 1b, 2a, and 3f exhibited superior activity against Trypanosoma cruzi, with IC50 values of 19.2, 37.3, 68.7, and 24.7 \u00b5M, respectively, outperforming the reference drug benznidazole (IC50: 54.7 \u00b5M). Results: Compounds 1b and 3f showed excellent selectivity indices against Leishmania braziliensis, with SI values of 19 and 18, respectively, suggesting they could be potential alternatives to the commonly used, but more selective, miltefosine (IC50: 64.0 \u00b5M, SI: 43.0). Additionally, compounds 1a, 1b, and 3f were most effective against Leishmania infantum, with IC50 values of 24.9, 30.5, and 46.6 \u00b5M, respectively. Compounds 3f and 3h were particularly potent against various Mycobacterium abscessus strains, highlighting their significance given the inherent resistance of these bacteria to standard antimicrobials. Conclusions: The sensitivity of Mycobacterium intracellulare LIID-01 to these compounds also underscored their potential in managing infections by the Mycobacterium avium\u2013intracellulare complex.<\/jats:p>","DOI":"10.3390\/ph17101375","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T09:14:48Z","timestamp":1729242888000},"page":"1375","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exploring Benzo[h]chromene Derivatives as Agents against Protozoal and Mycobacterial Infections"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0203-4891","authenticated-orcid":false,"given":"Mariano Walter","family":"Pertino","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica de Recursos Naturales, Universidad de Talca, Campus Lircay, Talca 3480094, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"F. de la Torre","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9228-5378","authenticated-orcid":false,"given":"Guillermo","family":"Schmeda-Hirschmann","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica de Recursos Naturales, Universidad de Talca, Campus Lircay, Talca 3480094, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-4316","authenticated-orcid":false,"given":"Celeste","family":"Vega G\u00f3mez","sequence":"additional","affiliation":[{"name":"Centro para el Desarrollo de la Investigaci\u00f3n Cient\u00edfica (CEDIC), Manduvir\u00e1 635, Asunci\u00f3n CP 1255, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8123-4260","authenticated-orcid":false,"given":"Miriam","family":"Rol\u00f3n","sequence":"additional","affiliation":[{"name":"Centro para el Desarrollo de la Investigaci\u00f3n Cient\u00edfica (CEDIC), Manduvir\u00e1 635, Asunci\u00f3n CP 1255, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6906-7495","authenticated-orcid":false,"given":"Cathia","family":"Coronel","sequence":"additional","affiliation":[{"name":"Centro para el Desarrollo de la Investigaci\u00f3n Cient\u00edfica (CEDIC), Manduvir\u00e1 635, Asunci\u00f3n CP 1255, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6207-1870","authenticated-orcid":false,"given":"Antonieta","family":"Rojas de Arias","sequence":"additional","affiliation":[{"name":"Centro para el Desarrollo de la Investigaci\u00f3n Cient\u00edfica (CEDIC), Manduvir\u00e1 635, Asunci\u00f3n CP 1255, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5128-0338","authenticated-orcid":false,"given":"Carmen A.","family":"Molina-Torres","sequence":"additional","affiliation":[{"name":"Servicios de Dermatolog\u00eda, Hospital Universitario \u201cJos\u00e9 E. Gonz\u00e1lez\u201d, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 64460, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucio","family":"Vera-Cabrera","sequence":"additional","affiliation":[{"name":"Servicios de Dermatolog\u00eda, Hospital Universitario \u201cJos\u00e9 E. Gonz\u00e1lez\u201d, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 64460, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-7972","authenticated-orcid":false,"given":"Ezequiel","family":"Viveros-Valdez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0140-6736(09)61422-7","article-title":"Socioeconomic aspects of neglected tropical diseases","volume":"375","author":"Conteh","year":"2010","journal-title":"Lancet"},{"key":"ref_2","unstructured":"Pan American Health Organization (2007). Health in the Americas 2007. Regional, Scientific and Technical Publication 622, Pan American Health Organization."},{"key":"ref_3","unstructured":"World Health Organization (2010). First WHO Report on Neglected Tropical Diseases. Working to Overcome the Global Impact Neglected Tropical Diseases, World Health Organization."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0065-308X(05)61004-4","article-title":"Control of Chagas disease","volume":"61","author":"Yamagata","year":"2006","journal-title":"Adv. Parasitol."},{"key":"ref_5","unstructured":"World Health Organization (2014). Global Health Estimates 2014 Summary Tables: DALY by Cause, Age and Sex, by WHO Region, 2000\u20132012, World Health Organization. Available online: http:\/\/www.who.int\/news-room\/facts-in-pictures\/detail\/chagas-disease."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1093\/cid\/cix064","article-title":"Prevalence of Chagas disease in the Latin American-born population of Los Angeles","volume":"64","author":"Meymandi","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1021\/acsmedchemlett.0c00649","article-title":"Novel linker variants of Antileishmanial\/Antitubercular 7-substituted 2-nitroimidazooxazines offer enhanced solubility","volume":"12","author":"Thompson","year":"2021","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1016\/j.ejmech.2007.11.007","article-title":"Activity of a hydroxybibenzyl bryophyte constituent against Leishmania spp. and Trypanosoma cruzi: In silico, in vitro and in vivo activity studies","volume":"43","author":"Roldos","year":"2008","journal-title":"Eur. J. Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1007\/s00436-005-1300-3","article-title":"A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing beta-galactosidase","volume":"95","author":"Vega","year":"2005","journal-title":"Parasitol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"105906","DOI":"10.1016\/j.ijantimicag.2020.105906","article-title":"Searching for drugs for Chagas disease, leishmaniasis and schistosomiasis: A review","volume":"55","author":"Santos","year":"2020","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1517\/17460441.2013.845554","article-title":"Design or screening of drugs for the treatment of Chagas disease: What shows the most promise?","volume":"8","author":"Lepesheva","year":"2013","journal-title":"Expert Opin. Drug Discov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.ejmech.2018.06.040","article-title":"Current advances in drug discovery for Chagas disease","volume":"155","author":"Scarim","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5046","DOI":"10.1016\/j.bmc.2011.06.023","article-title":"Trimethoxy-chalcone derivatives inhibit growth of Leishmania braziliensis: Synthesis, biological evaluation, molecular modeling and structure-activity relationship (SAR)","volume":"19","author":"Lamim","year":"2011","journal-title":"Bioorg. Med. Chem."},{"key":"ref_14","unstructured":"WHO (2012). Research and Development to Meet Health Needs in Developing Countries; Report of the Consultative Expert Working Group on Research and Development: Strengthening Global Financing and Coordination; Financing and Coordination, World Health Organization."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.mcna.2022.08.001","article-title":"Testing and Treating Mycobacterium tuberculosis Infection","volume":"106","author":"Menzies","year":"2022","journal-title":"Med. Clin. N. Am."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Iskandar, K., Murugaiyan, J., Hammoudi Halat, D., Hage, S.E., Chibabhai, V., Adukkadukkam, S., Roques, C., Molinier, L., Salameh, P., and Van Dongen, M. (2022). Antibiotic discovery and resistance: The chase and the race. Antibiotics, 11.","DOI":"10.3390\/antibiotics11020182"},{"key":"ref_17","unstructured":"World Health Organization (2024, October 10). Available online: https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/329368\/9789241565714-eng.pdf?ua=1."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/s41579-020-0331-1","article-title":"Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus","volume":"18","author":"Johansen","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1021\/ja01298a033","article-title":"The constitution of lapachol and its derivatives. Part V. The structure of paternd\u2019s \u201cIsolapachone\u201d","volume":"58","author":"Hooker","year":"1936","journal-title":"J. Am. Chem. Soc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19591","DOI":"10.1039\/C8NJ03699C","article-title":"An efficient cyclization of lapachol to new benzo[h]chromene hybrid compounds: A stepwise vs. one-pot esterification-click (CuAAC) study","volume":"42","author":"Ali","year":"2018","journal-title":"New J. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1016\/j.ejmech.2007.10.015","article-title":"Naphthoquinoidal [1,2,3]-triazole, a new structural moiety active against Trypanosoma cruzi","volume":"43","author":"Pinto","year":"2008","journal-title":"Eur. J. Med. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.ejmech.2012.03.039","article-title":"On the search for potential anti-Trypanosoma cruzi drugs: Synthesis and biological evaluation of 2-hydroxy-3-methylamino and 1,2,3-triazolic naphthoquinoidal compounds obtained by click chemistry reactions","volume":"52","author":"Diogo","year":"2012","journal-title":"Eur. J. Med. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.1016\/j.bmc.2010.03.029","article-title":"The evaluation of quinonoid compounds against Trypanosoma cruzi: Synthesis of imidazolic anthraquinones, nor-\u03b2-lapachone derivatives and \u03b2-lapachone-based 1,2,3-triazoles","volume":"18","author":"Pinto","year":"2010","journal-title":"Bioorg. Med. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6337","DOI":"10.1016\/j.bmc.2013.08.055","article-title":"Synthesis and anti-Trypanosoma cruzi activity of naphthoquinone-containing triazoles: Electrochemical studies on the effects of the quinoidal moiety","volume":"21","author":"Diogo","year":"2013","journal-title":"Bioorg. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.ejmech.2013.02.038","article-title":"Potent naphthoquinones against antimony-sensitive and -resistant Leishmania parasites: Synthesis of novel \u03b1- and nor-\u03b1-lapachone based 1,2,3-triazoles by copper-catalyzed azide-alkyne cycloaddition","volume":"63","author":"Pinto","year":"2013","journal-title":"Eur. J. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pertino, M.W., de la Torre, A.F., Schmeda-Hirschmann, G., Vega, C., Rolon, M., Coronel, C., Rojas de Arias, A., Leal Lopez, K., Carranza-Rosales, P., and Viveros Valdez, E. (2020). Synthesis, trypanocidal and anti-leishmania activity of new triazole-lapachol and nor-lapachol hybrids. Bioorg. Chem., 103.","DOI":"10.1016\/j.bioorg.2020.104122"},{"key":"ref_27","first-page":"1","article-title":"Impaired development of a miltefosine-resistant Leishmania infantum strain in the sand fly vectors Phlebotomus perniciosus and Lutzomyia longipalpis","volume":"11","author":"Suau","year":"2019","journal-title":"IJP Drugs Drug Resist."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/cid\/cit102","article-title":"Increasing failure of miltefosine in the treatment of Kalaazar in Nepal and the potential role of parasite drug resistance, reinfection, or noncompliance","volume":"56","author":"Rijal","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","first-page":"255","article-title":"Comprehensive coverage on anti-mycobacterial endeavour reported during 2022","volume":"5","author":"Dhameliya","year":"2023","journal-title":"Eur. J. Med. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1039\/b212154a","article-title":"Antimycobacterial natural products","volume":"20","author":"Copp","year":"2003","journal-title":"Nat. Prod. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/j.intimp.2010.08.023","article-title":"The hydroxy-naphthoquinone lapachol arrests mycobacterial growth and immunomodulates host macrophages","volume":"10","author":"Oliveira","year":"2010","journal-title":"Int. Immunopharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1089\/mdr.2020.0164","article-title":"Antituberculosis activities of lapachol and \u03b2-lapachone in combination with other drugs in acidic pH","volume":"27","author":"Ieque","year":"2021","journal-title":"Microb. Drug Resist."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/ddr.21468","article-title":"Microbial esterases and ester prodrugs: An unlikely marriage for combating antibiotic resistance","volume":"80","author":"Larsen","year":"2019","journal-title":"Drug Dev. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.eimc.2009.06.010","article-title":"F\u00e1rmacos con actividad frente a Mycobacterium tuberculosis [Active drugs against Mycobacterium tuberculosis]","volume":"27","author":"Coll","year":"2009","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1016\/j.drudis.2018.04.001","article-title":"NTM drug discovery: Status, gaps and the way forward","volume":"23","author":"Wu","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_36","unstructured":"Akram, S.M., and Attia, F.N. (2024, October 10). Mycobacterium avium Complex, StatPearls [Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK431110\/#."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Lin, S., Hua, W., Wang, S., Zhang, Y., Chen, X., Liu, H., Shao, L., Chen, J., and Zhang, W. (2022). In vitro assessment of 17 antimicrobial agents against clinical Mycobacterium avium complex isolates. BMC Microbiol., 22.","DOI":"10.1186\/s12866-022-02582-2"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"104794","DOI":"10.1016\/j.idnow.2023.104794","article-title":"Treatment of Resistant Gram-negative bacilli in children","volume":"53","author":"Cohen","year":"2023","journal-title":"Infect. Dis. Now"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zhou, G., Wang, Q., Wang, Y., Wen, X., Peng, H., Peng, R., Shi, Q., Xie, X., and Li, L. (2023). Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria. Microorganisms, 11.","DOI":"10.3390\/microorganisms11071690"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s00044-023-03163-2","article-title":"Design, synthesis and antimicrobial activities of 1,2,3-triazole hybrids with amine-ester functionality","volume":"33","author":"Sangwan","year":"2024","journal-title":"Med. Chem. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/nrd4609","article-title":"An analysis of the attrition of drug candidates from four major pharmaceutical companies","volume":"14","author":"Waring","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1021\/tx200211v","article-title":"Improving drug candidates by design: A focus on physicochemical properties as a means of improving compound disposition and safety","volume":"24","author":"Meanwell","year":"2011","journal-title":"Chem. Res. Toxicol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3714","DOI":"10.1021\/jm000942e","article-title":"Fast calculation of molecular polar surface area as a sum of fragment-based contributions and its application to the prediction of drug transport properties","volume":"43","author":"Ertl","year":"2000","journal-title":"J. Med. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Daina, A., Michielin, O., and Zoete, V. (2017). SwissADME: A free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules. Sci. Rep., 7.","DOI":"10.1038\/srep42717"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1023\/A:1015810312465","article-title":"Predicting skin permeability","volume":"9","author":"Potts","year":"1992","journal-title":"Pharm. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S1383-5769(99)00020-3","article-title":"A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue","volume":"48","author":"Mikus","year":"2000","journal-title":"Parasitol. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ijantimicag.2006.02.025","article-title":"Selective activity of polyene macrolides produced by genetically modified Streptomyces on Trypanosoma cruzi","volume":"28","author":"Seco","year":"2006","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1128\/jcm.31.2.175-178.1993","article-title":"Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis","volume":"31","author":"Telenti","year":"1993","journal-title":"J. Clin. Microbiol."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/10\/1375\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:14:09Z","timestamp":1760112849000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/10\/1375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,16]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["ph17101375"],"URL":"https:\/\/doi.org\/10.3390\/ph17101375","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,16]]}}}