{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:33:12Z","timestamp":1760146392132,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education","award":["INST189"],"award-info":[{"award-number":["INST189"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: This study investigated the ability of Enterococcus lactis (E. lactis) and Hasawi rice protein lysate (HPL) to suppress colitis induced by dextran sulfate sodium (DSS) in miceColitis is characterized by inflammation of the colon, and exploring potential therapeutic agents could lead to improved management strategies. Methods: Male mice were subjected to DSS treatment to induce colitis, followed by supplementation with E. lactis and\/or HPL. The study assessed various parameters, including disease activity index (DAI) scores, gut permeability measured using FITC-dextran, and superoxide dismutase (SOD) activity in excised colon tissues from both treated and untreated control groups. Results: E. lactis supplementation significantly alleviated DSS-induced colitis, as evidenced by improved DAI scores and enhanced gut permeability. Notably, E. lactis combined with HPL (0.1 mg\/108) exhibited superior tolerance to a 0.5% pancreatin solution compared to E. lactis alone. Both E. lactis and the combination treatment significantly increased SOD activity (5.6 \u00b1 0.23 SOD U\/mg protein for E. lactis and 6.7 \u00b1 0.23 SOD U\/mg protein for the combination) relative to the Azoxymethane (AOM)\/DSS group, suggesting a reduction in oxidative stress. Additionally, pro-inflammatory markers were significantly reduced in the group receiving both E. lactis and HPL compared to the E. lactis-only group. Levels of proteins associated with cell death, such as PCNA, PTEN, VEGF, COX-2, and STAT-3, were significantly decreased by 14.8% to 80% following E. lactis supplementation, with the combination treatment showing the most pronounced effects. Conclusions: These findings suggest E. lactis supplementation may be beneficial for colitis, with HPL potential to enhance its effectiveness.<\/jats:p>","DOI":"10.3390\/ph17111463","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T12:31:32Z","timestamp":1730377892000},"page":"1463","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4272-929X","authenticated-orcid":false,"given":"Ashraf","family":"Khalifa","sequence":"first","affiliation":[{"name":"Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia"},{"name":"Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayyadah Abdullah","family":"Alkuwayti","sequence":"additional","affiliation":[{"name":"Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basem M.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enas M.","family":"Ali","sequence":"additional","affiliation":[{"name":"Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairul Islam M.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia"},{"name":"Molecular Biology Division, Pondicherry Centre for Biological Sciences and Educational Trust, Pondicherry 605004, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shang, F., Jiang, X., Wang, H., Guo, S., Kang, S., Xu, B., Wang, X., Chen, S., Li, N., and Liu, B. (2024). Bifidobacteriumlongum suppresses colorectal cancer through the modulation of intestinal microbes and immune function. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1327464"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7407190","DOI":"10.1155\/2019\/7407190","article-title":"Mechanisms of Metastasis in Colorectal Cancer and Metastatic Organotropism: Hematogenous versus Peritoneal Spread","volume":"2019","author":"Pretzsch","year":"2019","journal-title":"J. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev-micro-102215-095513","article-title":"Gut Microbiota, Inflammation, and Colorectal Cancer","volume":"70","author":"Brennan","year":"2016","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_4","unstructured":"Rajendran, B., Manickam, V., Tamizhselvi, R., PrabhuDass, J.F., Iyer, S.K., Thirugnanasambantham, K., and Ibrahim, H.-I.M. (2022). Azabicyclononane Derivative Downregulates the P38 MAP-Kinase Pathway in Colon Cancer through Apoptosis. Chem. Biol. Lett., 9."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"skad016","DOI":"10.1093\/jas\/skad016","article-title":"Enterococcus faecium from chicken feces improves chicken immune response and alleviates Salmonella infections: A pilot study","volume":"101","author":"Khalifa","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3831","DOI":"10.2174\/138161212802083707","article-title":"Molecular link mechanisms between inflammation and cancer","volume":"18","author":"Carvalho","year":"2012","journal-title":"Curr. Pharm. Des."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1136\/gut.2005.070896","article-title":"5-Aminosalicylate use and colorectal cancer risk in inflammatory bowel disease: A large epidemiological study","volume":"54","author":"Card","year":"2005","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1177\/0018578718823736","article-title":"Role of anti-inflammatory drugs in the colorectal cancer","volume":"55","author":"Sada","year":"2020","journal-title":"Hosp. Pharm."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Kaewarsar, E., Chaiyasut, C., Lailerd, N., Makhamrueang, N., Peerajan, S., and Sirilun, S. (2023). Optimization of Mixed Inulin, Fructooligosaccharides, and Galactooligosaccharides as Prebiotics for Stimulation of Probiotics Growth and Function. Foods, 12.","DOI":"10.3390\/foods12081591"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Khalifa, A., Ibrahim, H.-I.M., Sheikh, A., and Khalil, H.E. (2023). Attenuation of Immunogenicity in MOG-Induced Oligodendrocytes by the Probiotic Bacterium Lactococcus sp. PO3. Medicina, 59.","DOI":"10.3390\/medicina59101731"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Alsaud, N., Almajed, A., Lwusaybie, A., Alsubaie, A., Alobaidan, H., Alessa, J., Almousa, A., Ibrahim, H.I.M., and Khalifa, A. (2023). The Halotolerant Probiotic Bacterium Enterococcus lactis ASF-2 from Al-Asfar Lake, Saudi Arabia, Reduces Inflammation in Carrageenan-Induced Paw Edema. Microorganisms, 11.","DOI":"10.3390\/microorganisms11102415"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Khalifa, A., Sheikh, A., and Ibrahim, H.I.M. (2022). Bacillus amyloliquefaciens Enriched Camel Milk Attenuated Colitis Symptoms in Mice Model. Nutrients, 14.","DOI":"10.3390\/nu14091967"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ibrahim, H.I.M., Sheikh, A., Khalil, H.E., and Khalifa, A. (2023). Bacillus amyloliquifaciens-Supplemented Camel Milk Suppresses Neuroinflammation of Autoimmune Encephalomyelitis in a Mouse Model by Regulating Inflammatory Markers. Nutrients, 15.","DOI":"10.3390\/nu15030550"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"112018","DOI":"10.1016\/j.plantsci.2024.112018","article-title":"Multitrait engineering of Hassawi red rice for sustainable cultivation","volume":"341","author":"Sedeek","year":"2024","journal-title":"Plant Sci."},{"key":"ref_15","first-page":"54","article-title":"Nutrient Components and Antioxidant Activity in Hassawi Rice and Basmati Rice Varieties","volume":"22","author":"Alqurashi","year":"2021","journal-title":"Sci. J. King Faisal Univ. Basic Appl. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s10681-014-1134-0","article-title":"New quantitative trait loci for enhancing adaptation to salinity in rice from Hasawi, a Saudi landrace into three African cultivars at the reproductive stage","volume":"200","author":"Bimpong","year":"2014","journal-title":"Euphytica"},{"key":"ref_17","first-page":"1","article-title":"Comparison of chemical composition, antioxidant, and bioactive compounds contents in different Hassawi and basmati rice genotypes","volume":"13","author":"Hadid","year":"2012","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Elkholy, S., Maher, S., El-Hamid, N., El-Sayed, H., Hassan, W., Abdelmaogood, A., Hussein, S., Jaremko, M., Alshawwa, S., and Alharbi, H. (2023). The immunomodulatory effects of probiotics and azithromycin in dextran sodium sulfate-induced ulcerative colitis in rats via TLR4-NF-\u03baB and p38-MAPK pathway. Biomed. Pharmacother., 165.","DOI":"10.1016\/j.biopha.2023.115005"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Raheem, A., Liang, L., Zhang, G., and Cui, S. (2021). Modulatory Effects of Probiotics During Pathogenic Infections with Emphasis on Immune Regulation. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.616713"},{"key":"ref_20","unstructured":"FAO\/WHO (2002). Report of a Joint FAO\/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. Guidelines for the Evaluation of Probiotics in Food, Food and Agriculture Organization\/World Health Organization. Available online: https:\/\/www.mhlw.go.jp\/file\/05-Shingikai-11121000-Iyakushokuhinkyoku-Soumuka\/0000197343.pdf."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nrmicro1840","article-title":"Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria","volume":"6","author":"Wells","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Saji, N., Francis, N., Blanchard, C.L., Schwarz, L.J., and Santhakumar, A.B. (2019). Rice bran phenolic compounds regulate genes associated with antioxidant and anti-Inflammatory activity in human umbilical vein endothelial cells with induced oxidative stress. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194715"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3771","DOI":"10.1080\/10408398.2016.1164120","article-title":"Rice bran nutraceutics: A comprehensive review","volume":"57","author":"Sohail","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"S100","DOI":"10.3177\/jnsv.65.S100","article-title":"Dietary tricin suppresses inflammation-related colon carcinogenesis in mice","volume":"65","author":"Tanaka","year":"2019","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Agista, A.Z., Rusbana, T.B., Islam, J., Ohsaki, Y., Sultana, H., Hirakawa, R., Watanabe, K., Nochi, T., and Budijanto, S. (2021). Fermented rice bran supplementation prevents the development of intestinal fibrosis due to DSS-induced inflammation in mice. Nutrients, 13.","DOI":"10.3390\/nu13061869"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Xing, L., Fu, L., Cao, S., Yin, Y., Wei, L., and Zhang, W. (2022). The anti-inflammatory effect of bovine bone-gelatin-derived peptides in LPS-induced RAW264.7 macrophages cells and dextran sulfate sodium-induced C57BL\/6 mice. Nutrients, 14.","DOI":"10.3390\/nu14071479"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Commane, D.M., Hughes, R., Shortt, C., and Rowland, I. (2005). The Potential Mechanisms Involved in the Anti-Carcinogenic Action of Probiotics, Elsevier BV.","DOI":"10.1016\/j.mrfmmm.2005.02.027"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Delgado, N.C., Torres-Maravilla, E., Mayorga-Reyes, L., Mart\u00edn, R., Langella, P., P\u00e9rez-Past\u00e9n-Borja, R., S\u00e1nchez-Pardo, M.E., and Berm\u00fadez-Humar\u00e1n, L.G. (2021). Antioxidant and Anti-Inflammatory Properties of Probiotic Candidate Strains Isolated during Fermentation of Agave (Agave angustifolia Haw). Microorganisms, 9.","DOI":"10.3390\/microorganisms9051063"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00394-013-0506-x","article-title":"Lactobacillus GG-fermented milk prevents DSS-induced colitis and regulates intestinal epithelial homeostasis through activation of epidermal growth factor receptor","volume":"53","author":"Yoda","year":"2014","journal-title":"Eur. J. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.18632\/aging.102927","article-title":"Effects of the probiotic formulation SLAB51 in in vitro and in vivo Parkinson\u2019s disease models","volume":"12","author":"Castelli","year":"2020","journal-title":"Aging"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tsao, S.P., Nurrahma, B.A., Kumar, R., Wu, C.H., Yeh, T.H., Chiu, C.C., Lee, Y.P., Liao, Y.C., Huang, C.H., and Yeh, Y.T. (2021). Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson\u2019s Disease Rats. Antioxidants, 10.","DOI":"10.3390\/antiox10111823"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Alkushi, A.G., Abdelfattah-Hassan, A., Eldoumani, H., Elazab, S.T., Mohamed, S.A.M., Metwally, A.S., SEl-Shetry, E., Saleh, A.A., ElSawy, N.A., and Ibrahim, D. (2022). Probiotics-loaded nanoparticles attenuated colon inflammation, oxidative stress, and apoptosis in colitis. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-08915-5"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/s41577-019-0268-7","article-title":"Host-microbiota interactions in inflammatory bowel disease","volume":"20","author":"Caruso","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1126\/science.aaa9420","article-title":"Mucosal Immunology. Individual intestinal symbionts induce a distinct population of ROR\u03b3 regulatory T cells","volume":"349","author":"Sefik","year":"2015","journal-title":"Science"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1002\/ibd.20543","article-title":"Effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B and signal transducers and activators of transcription in TNBS-induced colitis","volume":"14","author":"Kretzmann","year":"2008","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1002\/ibd.21420","article-title":"Role of different inflammatory and tumor biomarkers in the development of ulcerative colitis-associated carcinogenesis","volume":"17","author":"Talero","year":"2011","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kim, K.-T., Kim, J.W., Kim, S.I., Kim, S., Nguyen, T.H., and Kang, C.H. (2021). Antioxidant and anti-inflammatory effect and probiotic properties of lactic acid bacteria isolated from canine and feline feces. Microorganisms, 9.","DOI":"10.3390\/microorganisms9091971"},{"key":"ref_38","first-page":"325","article-title":"Preparation of enzymatic skim milk hydrolysates with low phenylalanine content","volume":"25","author":"Soares","year":"2006","journal-title":"Acta Farm. Bonaer."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1399-302X.2007.00402.x","article-title":"Characterization of oral lactobacilli as potential probiotics for oral health","volume":"23","author":"Marcotte","year":"2008","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Yasmin, I., Saeed, M., Khan, W.A., Khaliq, A., Chughtai, M.F.J., Iqbal, R., Tehseen, S., Naz, S., Liaqat, A., and Mehmood, T. (2020). In vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030354"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1007\/s12602-019-09610-0","article-title":"In Vitro Evaluation of Probiotic Potential and Safety Assessment of Lactobacillus mucosae Strains Isolated from Donkey\u2019s Lactation","volume":"12","author":"Rastogi","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.intimp.2017.09.015","article-title":"Enhancing miR-132 expression by aryl hydrocarbon receptor attenuates tumorigenesis associated with chronic colitis","volume":"52","author":"Alzahrani","year":"2017","journal-title":"Int. Immunopharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Kowalczyk, M., Or\u0142owski, M., Siermontowski, P., Mucha, D., Zinkiewicz, K., Kurpiewski, W., Zieli\u0144ski, E., Kowalczyk, I., and Pedrycz, A. (2018). Occurrence of colorectal aberrant crypt foci depending on age and dietary patterns of patients. BMC Cancer, 18.","DOI":"10.1186\/s12885-018-4100-8"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/11\/1463\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:25:42Z","timestamp":1760113542000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/11\/1463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,31]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["ph17111463"],"URL":"https:\/\/doi.org\/10.3390\/ph17111463","relation":{},"ISSN":["1424-8247"],"issn-type":[{"type":"electronic","value":"1424-8247"}],"subject":[],"published":{"date-parts":[[2024,10,31]]}}}