{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:09:37Z","timestamp":1784038177876,"version":"3.55.0"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T00:00:00Z","timestamp":1730592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Ministry of Health of the Czech Republic, AZV","award":["NU21-08-00510"],"award-info":[{"award-number":["NU21-08-00510"]}]},{"name":"The Ministry of Health of the Czech Republic, AZV","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]},{"name":"The Ministry of Health of the Czech Republic, AZV","award":["CZ.02.01.01\/00\/22_008\/0004596"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004596"]}]},{"name":"The Ministry of Education, Youth and Sports OPVVV PO1 project CEREBIT","award":["NU21-08-00510"],"award-info":[{"award-number":["NU21-08-00510"]}]},{"name":"The Ministry of Education, Youth and Sports OPVVV PO1 project CEREBIT","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]},{"name":"The Ministry of Education, Youth and Sports OPVVV PO1 project CEREBIT","award":["CZ.02.01.01\/00\/22_008\/0004596"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004596"]}]},{"name":"The Ministry of Education, Youth and Sports OP JAK","award":["NU21-08-00510"],"award-info":[{"award-number":["NU21-08-00510"]}]},{"name":"The Ministry of Education, Youth and Sports OP JAK","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]},{"name":"The Ministry of Education, Youth and Sports OP JAK","award":["CZ.02.01.01\/00\/22_008\/0004596"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004596"]}]},{"name":"Cooperation Program, research area IMMU (JT)","award":["NU21-08-00510"],"award-info":[{"award-number":["NU21-08-00510"]}]},{"name":"Cooperation Program, research area IMMU (JT)","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]},{"name":"Cooperation Program, research area IMMU (JT)","award":["CZ.02.01.01\/00\/22_008\/0004596"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004596"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for \u00b5CT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution \u00b5CT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by \u00b5CT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.<\/jats:p>","DOI":"10.3390\/ph17111479","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T03:57:34Z","timestamp":1730692654000},"page":"1479","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging"],"prefix":"10.3390","volume":"17","author":[{"given":"Jaroslav","family":"Tur\u00e1nek","sequence":"first","affiliation":[{"name":"Neurology Department, The International Clinical Research Center ICRC of St. Anne\u2019s University Hospital in Brno, Peka\u0159sk\u00e1 53, 656 91 Brno, Czech Republic"},{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hn\u011bvot\u00ednsk\u00e1 3, 775 15 Olomouc, Czech Republic"},{"name":"Institute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, Sokolsk\u00e1 581, 50005 Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavl\u00edna Tur\u00e1nek","family":"Kn\u00f6tigov\u00e1","sequence":"additional","affiliation":[{"name":"Neurology Department, The International Clinical Research Center ICRC of St. Anne\u2019s University Hospital in Brno, Peka\u0159sk\u00e1 53, 656 91 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Kulich","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, v.v.i., Hudcova 296\/70, 621 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0665-3873","authenticated-orcid":false,"given":"Radim","family":"Skoup\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Scientific Instruments, v.v.i., AS CR, Kr\u00e1lovopolsk\u00e1 147, 612 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Hrubanov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Scientific Instruments, v.v.i., AS CR, Kr\u00e1lovopolsk\u00e1 147, 612 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nad\u011b\u017eda","family":"Va\u0161kovicov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753\/5, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladislav","family":"Fekete","sequence":"additional","affiliation":[{"name":"Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 18200 Prague 8, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3871-3965","authenticated-orcid":false,"given":"Anton\u00edn","family":"Ka\u0148a","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technick\u00e1 5, 166 28 Praha 6, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Mikul\u00edk","sequence":"additional","affiliation":[{"name":"Neurology Department, The International Clinical Research Center ICRC of St. Anne\u2019s University Hospital in Brno, Peka\u0159sk\u00e1 53, 656 91 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8572-7246","authenticated-orcid":false,"given":"Milan","family":"Ra\u0161ka","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hn\u011bvot\u00ednsk\u00e1 3, 775 15 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6089","DOI":"10.1038\/s41467-020-19947-8","article-title":"Enhanced dispersion stability of gold nanoparticles by the physisorption of cyclic poly(ethylene glycol)","volume":"11","author":"Wang","year":"2020","journal-title":"Nat. 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