{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T17:32:21Z","timestamp":1768584741658,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vietnam National Foundation for Science and Technology Development (NAFOSTED)","award":["04\/2022\/TN"],"award-info":[{"award-number":["04\/2022\/TN"]}]},{"name":"Vietnam National Foundation for Science and Technology Development (NAFOSTED)","award":["IP-2020-02-2431"],"award-info":[{"award-number":["IP-2020-02-2431"]}]},{"name":"FM-AA (Funghi Medicinali Anti-Aging) Programma di Sviluppo Rurale per l\u2019Umbria 2014\u20132020\u2013Misura 16\u2014Sottomisura 16.2; Croatian Science Foundation","award":["04\/2022\/TN"],"award-info":[{"award-number":["04\/2022\/TN"]}]},{"name":"FM-AA (Funghi Medicinali Anti-Aging) Programma di Sviluppo Rurale per l\u2019Umbria 2014\u20132020\u2013Misura 16\u2014Sottomisura 16.2; Croatian Science Foundation","award":["IP-2020-02-2431"],"award-info":[{"award-number":["IP-2020-02-2431"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: The expression of oncogene zinc-finger protein 217 (ZNF217) has been reported to play a central role in cancer development, resistance, and recurrence. Therefore, targeting ZNF217 has been proposed as a possible strategy to fight cancer, and there has been much research on compounds that can target ZNF217. The present work investigates the chemo-preventive properties of cucurbitacin D, a compound with a broad range of anticancer effects, in hematological cancer cells, specifically with regard to its ability to modulate ZNF217 expression. Methods: Different cucurbitacins were isolated from the Vietnamese plant Elaeocarpus hainanensis. The purified compounds were tested on nucleophosmin-mutated acute myeloid leukemia and other hematological cancer cell lines to assess their effects on the cell cycle, cell viability and apoptosis, and the expression of ZNF217. Results: Cucurbitacin D resulted in a reduction in the number of acute myeloid leukemia cells by inducing an increase in apoptosis and blocking cell cycle progression. It also led to a significant decrease in ZNF217 expression in the nucleophosmin-mutated acute myeloid leukemia cell line but not in the other hematologic cancer cell lines. The reduction in ZNF217 expression contributed significantly to the blocking of cell cycle progression but did not affect apoptosis. Conclusions: The obtained results suggest that cucurbitacin D is a promising molecule for targeting mutated nucleophosmin or its pathway in acute myeloid leukemia cells, although further studies are needed for in-depth investigations into its specific mechanisms.<\/jats:p>","DOI":"10.3390\/ph17121561","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T06:11:54Z","timestamp":1732169514000},"page":"1561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Modulatory Effect of Cucurbitacin D from Elaeocarpus hainanensis on ZNF217 Oncogene Expression in NPM-Mutated Acute Myeloid Leukemia"],"prefix":"10.3390","volume":"17","author":[{"given":"Sabrina","family":"Adorisio","sequence":"first","affiliation":[{"name":"Section of Pharmacology, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3078-9754","authenticated-orcid":false,"given":"Alessandra","family":"Fierabracci","sequence":"additional","affiliation":[{"name":"Bambino Ges\u00f9 Children\u2019s Hospital, IRCCS, 00165 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ba Thi","family":"Cham","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam"},{"name":"Institute of Chemistry, VAST, Hanoi 10072, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2112-6732","authenticated-orcid":false,"given":"Vu Dinh","family":"Hoang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Hanoi 10000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi","family":"Thuy Linh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam"},{"name":"Institute of Chemistry, VAST, Hanoi 10072, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Thi Hong","family":"Nhung","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Technology, Hanoi University of Industry, Hanoi 10000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9139-1729","authenticated-orcid":false,"given":"Maria Paola","family":"Martelli","sequence":"additional","affiliation":[{"name":"Hematology, Department of Medicine and Surgery, University of Perugia and \u2018Santa Maria della Misericordia\u2019 Perugia Hospital, 06123 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emira","family":"Ayroldi","sequence":"additional","affiliation":[{"name":"Section of Pharmacology, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-4243","authenticated-orcid":false,"given":"Simona","family":"Ronchetti","sequence":"additional","affiliation":[{"name":"Section of Pharmacology, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucrezia","family":"Rosati","sequence":"additional","affiliation":[{"name":"Section of Pharmacology, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3049-6644","authenticated-orcid":false,"given":"Silvia","family":"Di Giacomo","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology \u201cV. Erspamer\u201d, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy"},{"name":"Department of Food Safety, Nutrition and Veterinary Public Health, Italian National Institute of Health, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-6925","authenticated-orcid":false,"given":"Trinh Thi","family":"Thuy","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10072, Vietnam"},{"name":"Institute of Chemistry, VAST, Hanoi 10072, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1792-9468","authenticated-orcid":false,"given":"Domenico Vittorio","family":"Delfino","sequence":"additional","affiliation":[{"name":"Section of Pharmacology, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"},{"name":"Foligno Nursing School and Master in Physiotherapy in Musculoskeletal and Rheumatological Area, Department of Medicine and Surgery, University of Perugia, 06129 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gyamfi, J., Kim, J., and Choi, J. 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