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(CNPq)","award":["405940\/2021-1"],"award-info":[{"award-number":["405940\/2021-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: This study evaluates the effect of Mn(II) and Co(II) ions on the production of anti-Candida metabolites by the endophytic fungus Aspergillus sp., isolated from Dizygostemon riparius. The objective was to identify metal-induced secondary metabolites with antifungal potential against drug-resistant Candida species. Methods: Aspergillus sp. was cultivated in Czapek agar supplemented with MnCl\u2082 (400 \u00b5M) or CoCl\u2082 (200 \u00b5M). Metabolite profiles were analyzed using UHPLC-DAD and LC-ESI-HRMS, followed by structural elucidation via NMR. Antifungal and biofilm inhibition activities were tested against Candida albicans and Candida parapsilosis. Toxicity was assessed using Tenebrio molitor larvae. Results: Key metabolites, including pyrophen, penicillquei B, and fonsecinone B, demonstrated antifungal activity with MIC values of 4.37\u2013280.61 \u00b5g\/mL. Fonsecinone B exhibited superior biofilm inhibition, surpassing fluconazole in reducing biofilm biomass and viability. In vivo assays showed low toxicity, with survival rates above 80% at 2\u00d7 MIC\/kg. Conclusions: Mn(II) and Co(II) significantly modulated the production of antifungal metabolites in Aspergillus sp. Fonsecinone B emerged as a promising candidate for antifungal therapy due to its potent activity and low toxicity. These findings support further investigation into the therapeutic potential of metal-induced fungal metabolites for combating drug-resistant Candida infections.<\/jats:p>","DOI":"10.3390\/ph17121678","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T09:38:15Z","timestamp":1733996295000},"page":"1678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Mn(II) and Co(II) on Anti-Candida Metabolite Production by Aspergillus sp. an Endophyte Isolated from Dizygostemon riparius (Plantaginaceae)"],"prefix":"10.3390","volume":"17","author":[{"given":"Anne Karoline Maiorana","family":"Santos","sequence":"first","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Ara\u00fajo","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josivan Regis","family":"Farias","sequence":"additional","affiliation":[{"name":"Center for Biological and Health Sciences, Federal University of Maranh\u00e3o, S\u00e3o Lu\u00eds 65080-805, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e3o Vieira","family":"de Morais","sequence":"additional","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleydlenne Costa","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-9262","authenticated-orcid":false,"given":"Rosane Nassar Meireles","family":"Guerra","sequence":"additional","affiliation":[{"name":"Center for Biological and Health Sciences, Federal University of Maranh\u00e3o, S\u00e3o Lu\u00eds 65080-805, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Rodrigues-Filho","sequence":"additional","affiliation":[{"name":"LaBioMMi, Federal University of S\u00e3o Carlos, CP 676, S\u00e3o Carlos 13565-905, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5267-9500","authenticated-orcid":false,"given":"Alberto Jorge Oliveira","family":"Lopes","sequence":"additional","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2009-2817","authenticated-orcid":false,"given":"Ant\u00f4nio Jos\u00e9","family":"Cantanhede Filho","sequence":"additional","affiliation":[{"name":"Chemistry Postgraduate Program, Federal Institute of Science Education and Technology of Maranh\u00e3o, S\u00e3o Lu\u00eds 65030-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/s41579-018-0121-1","article-title":"Fungal Secondary Metabolism: Regulation, Function and Drug Discovery","volume":"17","author":"Keller","year":"2019","journal-title":"Nat. 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