{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T13:17:13Z","timestamp":1763644633808,"version":"build-2065373602"},"reference-count":94,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fiocruz"},{"name":"ICC\/Fiocruz PR"},{"name":"CNPq"},{"name":"CAPES"},{"name":"Funda\u00e7\u00e3o Arauc\u00e1ria"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/objectives: In Brazil, the co-circulation of arboviruses\u2014such as dengue, Zika, yellow fever, and Chikungunya viruses\u2014creates a complex epidemiological landscape, drawing attention from health authorities due to high morbidity and mortality rates. Also present in this context is the Mayaro virus (MAYV), a neglected arbovirus, which can also cause severe syndromes and has been expanding beyond its usual endemic areas in northern and central-western Brazil. Epidemiological surveillance measures remain limited, and there are no effective prophylactic strategies or antiviral treatments for this neglected arbovirus. In this study, we evaluated the antiviral activity of commercial and synthetic compounds against MAYV using an image high-throughput screening (iHTS) system. Methods: A total of 52 compounds from an FDA-approved commercial library (Tocriscreen) and 50 other compounds were tested. Results: Seven compounds showed anti-MAYV activity and were non-toxic for the following cell lines: Naringenin, LLA9A, chrysin, and its ester C6. Post-infection treatments with these selected compounds significantly decreased the percentage of infected cells and the release of infectious viral particles in the supernatant. Additionally, anti-MAYV activity of these four selected hits was confirmed using several human cell lines and two different MAYV genotypes. Conclusions: Our results indicate that the iHTS platform is effective for screening anti-MAYV drugs and that four promising compounds can efficiently inhibit MAYV replication in human cell lines. Although in vivo studies are still required to confirm the efficacy of the selected hits, our findings provide a starting point for developing a potential treatment for MAYV infections.<\/jats:p>","DOI":"10.3390\/ph18050717","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:59:29Z","timestamp":1747195169000},"page":"717","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9769-3699","authenticated-orcid":false,"given":"Ana Paula","family":"Andreolla","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Instituto Carlos Chagas, ICC\/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea Cristine","family":"Koishi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Instituto Carlos Chagas, ICC\/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7167-4711","authenticated-orcid":false,"given":"Alessandra Abel","family":"Borges","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisas em Virologia e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade (ICBS), Universidade Federal de Alagoas (UFAL), Av. Lourival Melo Mota, s\/n, Tabuleiro do Martins, Macei\u00f3 57072-900, Alagoas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Albuquerque de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviane Guedes de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Educa\u00e7\u00e3o, Agricultura e Ambiente, IEAA, Universidade Federal do Amazonas, Rua 29 de agosto, Centro, Humait\u00e1 69800\u2013000, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9669-0306","authenticated-orcid":false,"given":"Nerilson Marques","family":"Lima","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7039-4090","authenticated-orcid":false,"given":"Eloah Pereira","family":"\u00c1vila","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3018-3130","authenticated-orcid":false,"given":"Pedro P\u00f4ssa","family":"de Castro","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1004-5395","authenticated-orcid":false,"given":"Giovanni Wilson","family":"Amarante","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1112-0801","authenticated-orcid":false,"given":"Mauro Vieira","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, ICE, Universidade Federal de Juiz de Fora, Rua Jos\u00e9 Louren\u00e7o Kelmer s\/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1965-6152","authenticated-orcid":false,"given":"Juliano","family":"Bordignon","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Instituto Carlos Chagas, ICC\/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Nunes","family":"Duarte dos Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Instituto Carlos Chagas, ICC\/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Aguilar-Luis, M.A., del Valle-Mendoza, J., Sandoval, I., Silva-Caso, W., Mazulis, F., Carrillo-Ng, H., Tarazona-Castro, Y., Martins-Luna, J., Aquino-Ortega, R., and Pe\u00f1a-Tuesta, I. 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