{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:27:21Z","timestamp":1790890041880,"version":"4.1.0"},"reference-count":76,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPEG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa no Estado de Goi\u00e1s)","award":["179\/2014-GR"],"award-info":[{"award-number":["179\/2014-GR"]}]},{"name":"Institutional Program for Support to Research of PUC Goi\u00e1s (PAP\/PUC Goi\u00e1s)","award":["179\/2014-GR"],"award-info":[{"award-number":["179\/2014-GR"]}]},{"name":"Program for Support of Scientific Publication","award":["179\/2014-GR"],"award-info":[{"award-number":["179\/2014-GR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background:\u00a0Eugenia involucrata DC., a Cerrado native plant, is recognized for its medicinal properties. However, its bioactive compounds remain inadequately explored. Objectives: This study investigated bioactive compounds from a standardized liquid extract from E. involucrata leaves that can act with antioxidant, cytogenotoxic, cytoprotective, and genoprotective effects. Methods: The phenolic compounds in the standardized liquid extract from E. involucrata leaves were screened by HPLC-DAD. The capture of the free radicals DPPH, ABTS+, and the metal reduction power FRAP determined the antioxidant potential. Cytotoxicity was evaluated in RAW 264.7 macrophages (MTT assay), and (anti)cytotoxic and (anti)genotoxic effects were assessed in human lymphocytes using the Trypan blue exclusion method and comet assay, respectively. Results: The extracts present key phenolic compounds, such as ellagic acid, myricitrin, and epicatechin gallate. The standardized extract demonstrated antioxidant capacity, evidenced by its ability to reduce iron and scavenge free radicals. The liquid extract from E. involucrata leaves exhibited cytotoxic effects on RAW 264.7 macrophages at higher concentrations, while demonstrating (anti)cytotoxic activity on human lymphocytes from all tested concentrations. The highest concentration tested of the standardized liquid extract from E. involucrata leaves (250 \u00b5g\/mL) showed genotoxicity against human lymphocytes compared to the negative control. In contrast, the lowest concentration (62.5 \u00b5g\/mL) exhibited an antigenotoxic effect on human lymphocytes, reducing the genotoxicity of doxorubicin by approximately 27%. Conclusions: The bioactive compounds in the standardized liquid extract from E. involucrata leaves exhibited antioxidant and antigenotoxic properties, suggesting potential value for nutraceutical and pharmaceutical applications, particularly those related to oxidative stress associated withaging and disease progression.<\/jats:p>","DOI":"10.3390\/ph18050764","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T09:58:22Z","timestamp":1747821502000},"page":"764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bioactive Compounds, Antioxidant, Cytotoxic, and Genotoxic Investigation of the Standardized Liquid Extract from Eugenia involucrata DC. Leaves"],"prefix":"10.3390","volume":"18","author":[{"given":"Monatha Nayara Guimar\u00e3es","family":"Te\u00f3filo","sequence":"first","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8356-7023","authenticated-orcid":false,"given":"Leonardo Gomes","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3439-0433","authenticated-orcid":false,"given":"Jamira Dias","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-5454","authenticated-orcid":false,"given":"Fernando Gomes","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anielly Monteiro de","family":"Melo","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4440-9636","authenticated-orcid":false,"given":"Grazzielle Guimar\u00e3es","family":"de Matos","sequence":"additional","affiliation":[{"name":"Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Maria Ascari","family":"Morgado","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Silva","family":"Fernandes","sequence":"additional","affiliation":[{"name":"School of Medical and Life Sciences, Pontifical Catholic University of Goi\u00e1s, Goi\u00e2nia 74605-010, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9365-7196","authenticated-orcid":false,"given":"Lucas Barbosa Ribeiro","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"School of Medical and Life Sciences, Pontifical Catholic University of Goi\u00e1s, Goi\u00e2nia 74605-010, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-8274","authenticated-orcid":false,"given":"Clayson Moura","family":"Gomes","sequence":"additional","affiliation":[{"name":"School of Medical and Life Sciences, Pontifical Catholic University of Goi\u00e1s, Goi\u00e2nia 74605-010, GO, Brazil"},{"name":"Faculty of Military Principles, Goi\u00e2nia 74120-020, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3444-6751","authenticated-orcid":false,"given":"Milton Adriano Pelli","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0737-2600","authenticated-orcid":false,"given":"Joelma Abadia Marciano de","family":"Paula","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa Fl\u00e1via Luiz Cardoso","family":"Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-3944","authenticated-orcid":false,"given":"Leonardo Luiz","family":"Borges","sequence":"additional","affiliation":[{"name":"Laboratory for Research, Development and Innovation of Biodiversity Products, State University of Goi\u00e1s, Central Campus, An\u00e1polis 75132-903, GO, Brazil"},{"name":"School of Medical and Life Sciences, Pontifical Catholic University of Goi\u00e1s, Goi\u00e2nia 74605-010, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jpba.2014.07.003","article-title":"Eugenia calycina Cambess Extracts and Their Fractions: Their Antimicrobial Activity and the Identification of Major Polar Compounds Using Electrospray Ionization FT-ICR Mass Spectrometry","volume":"99","author":"Ferreira","year":"2014","journal-title":"J. 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