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However, concerns about their potential toxicity have also emerged, particularly regarding their impact on human and animal health. This study investigates the acute toxicological effects of AgNPs synthesized using a green route with an aqueous extract of a native Cerrado plant (AgNPs-Cb) in mice. Methods: The AgNPs-Cb were intravenously administered at a concentration of 64 \u00b5M, and the mice were euthanized after 24 h for the collection of blood and organ samples (liver, spleen, kidneys, and lungs) for hematological, biochemical, and histological analyses. Results: Hematological analysis, including complete blood count (CBC) and differential leukocyte count, showed no statistically significant alterations in the groups treated with AgNPs-Cb, Cb extract, and Ag+, compared with the control group (p &lt; 0.05). Notably, only the Ag+ group exhibited a significant increase in red blood cell count and hematocrit levels, suggesting that the nanoformulation of silver might mitigate the hematological impact seen with free silver ions. Biochemical analyses of liver and kidney function markers also revealed no significant differences across the treatment groups. Conclusions: These findings indicate that AgNPs-Cb may offer a safer alternative for antimicrobial applications, reducing the risk of acute toxicity in mammals while maintaining efficacy against pathogens. Further studies are needed to explore the underlying mechanisms and long-term effects of AgNPs-Cb exposure.<\/jats:p>","DOI":"10.3390\/ph18070993","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T09:08:44Z","timestamp":1751447324000},"page":"993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Nanotoxicological Assessment of Green-Synthesized Silver Nanoparticles from Brazilian Cerrado Plant in a Murine Model"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2964-5550","authenticated-orcid":false,"given":"C\u00ednthia Caetano","family":"Bonatto","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"},{"name":"Laboratory of Nanobiotechnology (LNANO), Embrapa Genetic Resources and Biotechnology, Parque Estacao Biologica, Final W5 Norte, Bras\u00edlia 70770-917, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivy Garcez","family":"Reis","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology (LNANO), Embrapa Genetic Resources and Biotechnology, Parque Estacao Biologica, Final W5 Norte, Bras\u00edlia 70770-917, DF, Brazil"},{"name":"Institute of Biological Sciences, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalila Juliana Silva","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"},{"name":"Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel das Neves","family":"Almeida","sequence":"additional","affiliation":[{"name":"Neuroimmune Interactions Laboratory, Department of Neurology, UMass Chan Medical School, Worcester, MA 01655, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2989-2383","authenticated-orcid":false,"given":"Rafael","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia Pimentel Sant\u2019Ana","family":"Dourado","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Pasquarelli-do-Nascimento","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly Grace","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-7725","authenticated-orcid":false,"given":"Luciano Paulino","family":"Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Biology, University of Brasilia (UnB), Bras\u00edlia 70910-900, DF, Brazil"},{"name":"Laboratory of Nanobiotechnology (LNANO), Embrapa Genetic Resources and Biotechnology, Parque Estacao Biologica, Final W5 Norte, Bras\u00edlia 70770-917, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e36539","DOI":"10.1016\/j.heliyon.2024.e36539","article-title":"Preparation methods, applications, toxicity and mechanisms of silver nanoparticles as bactericidal agent and superiority of green synthesis method","volume":"10","author":"Elimbinzi","year":"2024","journal-title":"Heliyon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.tiv.2016.10.012","article-title":"Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies","volume":"38","author":"Nagy","year":"2017","journal-title":"Toxicol. 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