{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:33:12Z","timestamp":1760059992864,"version":"build-2065373602"},"reference-count":61,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Collaboration Project \u201cStrengthening and development of research capacities for the treatment of neglected and neglected parasitic diseases\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Leishmaniasis is a zoonotic and anthropozoonotic disease with significant public health impact worldwide and is classified as a neglected tropical disease. The search for new affordable treatments, particularly oral and\/or topical ones that are easy to administer and have fewer side effects, remains a priority for the scientific community in this field of research. In previous investigations, 3-alkoxy-1-benzyl-5-nitroindazole derivatives showed remarkable in vitro results against Leishmania species, and predictions of absorption, distribution, metabolism, excretion, and toxicity properties, as well as pharmacological scores, of the compounds classified as active were superior to those of amphotericin B, indicating their potential as candidates for in vivo studies. Therefore, the aim of the present study was to evaluate the in vivo antileishmanial activity of the indazole derivatives NV6 and NV16. Methods: The compounds were administered intralesionally at concentrations of 10 and 5 mg\/kg in a BALB\/c mouse model of cutaneous leishmaniasis caused by Leishmania amazonensis. To evaluate the efficacy of the compounds, indicators such as lesion size, ulcer area, lesion weight, and parasitic load were determined. Amphotericin B was used as a positive control. Results: The compound NV6 showed leishmanicidal activity comparable to that observed with amphotericin B, with a significant reduction in lesion development and parasite load, while NV16 caused a reduction in ulcer area. Conclusions: These results provide strong evidence for the antileishmanial activity of NV6 and support future studies to improve its pharmacokinetic profile, as well as the investigation of combination therapies with other chemotherapeutic agents currently in use.<\/jats:p>","DOI":"10.3390\/ph18081107","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T14:40:02Z","timestamp":1753454402000},"page":"1107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Indazole Derivatives Against Murine Cutaneous Leishmaniasis"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5137-0253","authenticated-orcid":false,"given":"Niurka","family":"Mollineda-Diogo","sequence":"first","affiliation":[{"name":"Centro de Bioactivos Qu\u00edmicos, Universidad Central \u201cMarta Abreu\u201d de Las Villas, Santa Clara 54800, Villa Clara, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3710-0035","authenticated-orcid":false,"given":"Yunierkis","family":"P\u00e9rez-Castillo","sequence":"additional","affiliation":[{"name":"Grupo de Bio-Quimioinform\u00e1tica and Facultad de Ingenier\u00eda y Ciencias Aplicadas, Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1226-8648","authenticated-orcid":false,"given":"Sergio","family":"Sifontes-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico\u2014Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas (CONAHCYT), Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osmani","family":"Marrero-Chang","sequence":"additional","affiliation":[{"name":"Centro de Bioactivos Qu\u00edmicos, Universidad Central \u201cMarta Abreu\u201d de Las Villas, Santa Clara 54800, Villa Clara, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Meneses-Marcel","sequence":"additional","affiliation":[{"name":"Centro de Bioactivos Qu\u00edmicos, Universidad Central \u201cMarta Abreu\u201d de Las Villas, Santa Clara 54800, Villa Clara, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0060-2939","authenticated-orcid":false,"given":"Alma Reyna","family":"Escalona-Monta\u00f1o","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n UNAM-INC, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico\u2014Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8696-9995","authenticated-orcid":false,"given":"Mar\u00eda Magdalena","family":"Aguirre-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n UNAM-INC, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico\u2014Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6435-4380","authenticated-orcid":false,"given":"Teresa","family":"Espinosa-Buitrago","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, Pza. Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"},{"name":"Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, Boadilla del Monte, 28660 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeny","family":"Morales-Moreno","sequence":"additional","affiliation":[{"name":"Centro de Bioactivos Qu\u00edmicos, Universidad Central \u201cMarta Abreu\u201d de Las Villas, Santa Clara 54800, Villa Clara, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3390-7157","authenticated-orcid":false,"given":"Vicente","family":"Ar\u00e1n-Red\u00f3","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica M\u00e9dica del Consejo Superior de Investigaciones Cient\u00edficas de Espa\u00f1a, Juan de la Cierva 3, 28006 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/0738-081X(96)00057-0","article-title":"Leishmaniasis: Public Health Aspects and Control","volume":"14","author":"Desjeux","year":"1996","journal-title":"Clin. 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