{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:16:44Z","timestamp":1788833804857,"version":"build-2803163510"},"reference-count":73,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: The search for new bioactive molecules increasingly extends beyond conventional medicinal plants, highlighting the importance of exploring alternative botanical sources. Parasitic plants represent a promising but underexploited reservoir of pharmacologically relevant compounds. Cuscuta australis (CA), a parasitic species with a history of traditional use, remains poorly characterized. This study aimed to investigate its phytochemical composition and evaluate its antioxidant, anti-inflammatory, and hepatoprotective properties. Methods: The phytochemical profile of CA extract was characterized by LC-MS. Antioxidant capacity was assessed using DPPH and ABTS assays. In vivo hepatoprotection was evaluated in male rats subjected to CCl4-induced hepatotoxicity and treated orally with CA (30 or 60 mg\/kg body weight). Biochemical, lipid, oxidative stress, and histological parameters were determined. Molecular docking was conducted to predict the binding of major identified compounds against selected protein targets. Results: CA significantly and dose-dependently improved biochemical and histological markers. At 60 mg\/kg, ALT, AST, ALP, and bilirubin were reduced by 32%, 33%, 63%, and 51%, respectively. Lipid metabolism was improved by decreased TC, TG, and LDL-C with increased HDL-C. Antioxidant defense was enhanced through elevated CAT, SOD, and GPx activities, accompanied by reduced MDA levels. TNF-\u03b1 and IL-6 decreased by 48% and 53%, respectively. Histopathology confirmed hepatoprotection and reduced fibrosis. Docking studies revealed strong binding affinities (\u22127.07 to \u221219.20 kcal\/mol) for several metabolites, notably quercetin glucoside, diosmetin glucoside, caffeic acid glucoside, feruloylquinic acid, and isorhamnetin glucoside, against CYP450, IL-2, TNF-\u03b1, and IL-6. Conclusions: These findings demonstrate that C. australis is a promising source of bioactive compounds with hepatoprotective, antioxidant, antihyperlipidemic, and anti-inflammatory effects, supporting its potential as a natural therapeutic agent.<\/jats:p>","DOI":"10.3390\/ph18101524","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:50:16Z","timestamp":1760107816000},"page":"1524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Protective Effects of Cuscuta australis Against CCl4-Induced Hepatic Injury in Rats: Antioxidant, Anti-Inflammatory, and In Silico Insights"],"prefix":"10.3390","volume":"18","author":[{"given":"Hanen","family":"Baccari","sequence":"first","affiliation":[{"name":"Field Crop Laboratory, National Institute for Agricultural Research of Tunisia, University of Carthage, ElMenzah I, Tunis 1004, Tunisia"},{"name":"Faculty of Sciences of Gafsa, University of Gafsa, Gafsa 2012, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8456-0372","authenticated-orcid":false,"given":"Arij","family":"Bedoui","sequence":"additional","affiliation":[{"name":"Field Crop Laboratory, National Institute for Agricultural Research of Tunisia, University of Carthage, ElMenzah I, Tunis 1004, Tunisia"},{"name":"Faculty of Sciences of Gafsa, University of Gafsa, Gafsa 2012, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anouar","family":"Feriani","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems, Faculty of Sciences of Gafsa, University of Gafsa, Gafsa 2112, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Bouallegue","sequence":"additional","affiliation":[{"name":"Field Crop Laboratory, National Institute for Agricultural Research of Tunisia, University of Carthage, ElMenzah I, Tunis 1004, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6035-4803","authenticated-orcid":false,"given":"Nihad","family":"Sahri","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, University Mohammed VI Polytechnic, Ben Guerir 43150, Morocco"},{"name":"Physio-Chemical Laboratory of Inorganic and Organic Materials, Materials Science Center, Ecole Normale Sup\u00e9rieure, Mohammed V University, Rabat 10001, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohaib","family":"Khatib","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, University Mohammed VI Polytechnic, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Kharrat","sequence":"additional","affiliation":[{"name":"Field Crop Laboratory, National Institute for Agricultural Research of Tunisia, University of Carthage, ElMenzah I, Tunis 1004, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1018-0730","authenticated-orcid":false,"given":"Nizar","family":"Tlili","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Sciences and Technologies, Higher Institute of Environmental Sciences and Technologies of Borj-Cedria, PB-1003, Hammam-Lif 2050, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2719-8534","authenticated-orcid":false,"given":"Mansour","family":"Sobeh","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, University Mohammed VI Polytechnic, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4707-0618","authenticated-orcid":false,"given":"Moez","family":"Amri","sequence":"additional","affiliation":[{"name":"AgroBioSciences Program, College of Agriculture and Environmental Sciences, University Mohammed VI Polytechnic, Ben Guerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4419-4639","authenticated-orcid":false,"given":"Zouhaier","family":"Abbes","sequence":"additional","affiliation":[{"name":"Field Crop Laboratory, National Institute for Agricultural Research of Tunisia, University of Carthage, ElMenzah I, Tunis 1004, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3138\/canlivj-2025-0006-fr","article-title":"Investir dans l\u2019avenir: Formation pour les soins cliniques et la recherche sur les maladies du foie","volume":"8","author":"Coffin","year":"2025","journal-title":"Can. Liver J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107252","DOI":"10.1016\/j.aquatox.2025.107252","article-title":"Hepatotoxic effects of environmentally relevant concentrations of polystyrene microplastics on senescent Zebrafish (Danio rerio): Patterns of stress response and metabolomic alterations","volume":"279","author":"Xie","year":"2025","journal-title":"Aquat. Toxicol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1515\/znc-2024-0116","article-title":"Hepatoprotective activity of medicinal plants, their phytochemistry, and safety concerns: A systematic review","volume":"80","author":"Gonfa","year":"2025","journal-title":"Z. F\u00fcr Naturforschung C"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/HM9.0000000000000160","article-title":"Research progress on the hepatoprotective effect, pharmacokinetic properties, and hepatotoxicity of geniposide","volume":"5","author":"Tang","year":"2025","journal-title":"Acupunct. Herb. Med."},{"key":"ref_5","first-page":"1309","article-title":"Formulation, Development and Evaluation of Plicosepalus Acacia Extract Capsules Delivery System as an Advanced Phytotherapy Approach for Hepatoprotective","volume":"14","author":"Alburyhi","year":"2025","journal-title":"World J. Pharm. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Lozanova, V., Teofanova, D., Chakarova, B., Rusanov, K., Pachedjieva, K., Tosheva, A., and Zagorchev, L. (2025). The Antioxidant Properties of Extracts of Cuscuta spp. Depend on the Parasite and the Host Species. Antioxidants, 14.","DOI":"10.3390\/antiox14070761"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/tax.12195","article-title":"Parasitic Angiosperms: How Often and How Many?","volume":"69","author":"Nickrent","year":"2020","journal-title":"Taxon"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/plphys\/kiab055","article-title":"Parasitic Plants: Physiology, Development, Signaling, and Ecosystem Interactions","volume":"185","author":"Bouwmeester","year":"2021","journal-title":"Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"152617","DOI":"10.1016\/j.flora.2024.152617","article-title":"Host-Driven Phenotypic and Phenological Differentiation in Sympatric Races of a Parasitic Plant","volume":"320","author":"Ortiz","year":"2024","journal-title":"Flora"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ramezan, D., Farrokhzad, Y., Zargar, M., Stybayev, G., Kipshakbayeva, G., and Baitelenova, A. (2023). An Insight into Cuscuta campestris as a Medicinal Plant: Phytochemical Variation of Cuscuta Campestris with Various Host Plants. Agriculture, 13.","DOI":"10.3390\/agriculture13040770"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1093\/plphys\/kiaa069","article-title":"The Bright Side of Parasitic Plants: What Are They Good For?","volume":"185","author":"Li","year":"2021","journal-title":"Plant Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kurt-Celep, I., Yagi, S., Dall\u2019Acqua, S., Sut, S., Celep, E., Kaya, S., Berisha, A., Ponnaiya, S.K.M., Balakrishnan, K., and Yildiztugay, E. (2024). From Parasitic Life to Health-Promoting Applications\u2014A Versatile Goldmine Discovered in Nature\u2019s Secret Treasure Chest: Orobanche Nana. Food Biosci., 62.","DOI":"10.1016\/j.fbio.2024.105296"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Wang, Y., Xu, J., Hu, B., Dong, C., Sun, J., Li, Z., Ye, K., Deng, F., Wang, L., and Aslam, M. (2023). Assembly, Annotation, and Comparative Analysis of Ipomoea Chloroplast Genomes Provide Insights into the Parasitic Characteristics of Cuscuta Species. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1074697"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1080\/14786419.2023.2297264","article-title":"The Chemical Composition of the Essential Oils of Two Mediterranean Species of Convolvulaceae: Convolvulus althaeoides subsp. Tenuissimus Collected in Sicily (Italy) and Calystegia silvatica Collected in Algeria","volume":"39","author":"Derbak","year":"2025","journal-title":"Nat. Prod. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Fischer, K., Lachner, L.A.-M., Olsen, S., Mulisch, M., and Krause, K. (2021). The Enigma of Interspecific Plasmodesmata: Insight from Parasitic Plants. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.641924"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pan, H., Zagorchev, L., Chen, L., Tao, Y., Cai, C., Jiang, M., Sun, Z., and Li, J. (2023). Complete Chloroplast Genomes of Five Cuscuta Species and Their Evolutionary Significance in the Cuscuta Genus. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09427-w"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"110966","DOI":"10.1016\/j.ygeno.2024.110966","article-title":"Unveiling the Intricate Structural Variability Induced by Repeat-Mediated Recombination in the Complete Mitochondrial Genome of Cuscuta gronovii Willd","volume":"117","author":"Yang","year":"2025","journal-title":"Genomics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.15835\/nbha48311997","article-title":"Molecular Phylogeny Based on Its Sequences of nrDNA of Some Species Belonging to Dodder (Cuscuta L.) Genus from Various Ecological Sites of Turkey","volume":"48","author":"Kaya","year":"2020","journal-title":"Not. Bot. Horti Agrobot. Cluj-Napoca"},{"key":"ref_19","first-page":"179","article-title":"Cuscuta campestris (Cuscutaceae) Une Holoparasite Nouvelle et Envahissante Pour La Flore de Tunisie","volume":"26","author":"Elaissi","year":"2016","journal-title":"Flora Mediterr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110458","DOI":"10.1016\/j.scienta.2021.110458","article-title":"Integrated Small RNA, mRNA, and Degradome Sequencing Reveals the Important Role of miRNAs in the Interactions between Parasitic Plant Cuscuta australis and Its Host Trifolium repens","volume":"289","author":"Zhou","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"197","DOI":"10.28978\/nesciences.1643498","article-title":"Detection of Phytoconstituents: Therapeutic, Nutritional and Industrial of Cuscuta Australis Seeds Parasitizing on Basil","volume":"10","year":"2025","journal-title":"NESciences"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Gangarde, P., Bhatt, S., and Pujari, R. (2025). Assessment of Neuroprotective Potential of Cuscuta reflexa in Aluminium Chloride-Induced Experimental Model of Alzheimer\u2019s Disease: In Vitro and In Vivo Studies. J. Trace Elem. Med. Biol., 88.","DOI":"10.1016\/j.jtemb.2025.127612"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101401","DOI":"10.1016\/j.conctc.2024.101401","article-title":"Efficacy of Ma\u2019aljobon Aftimouni (Cuscuta reflexa and Whey) on HbA1c and Blood Glucose Levels in Patients with Type 2 Diabetes: A Randomized Triple-Blind Clinical Trial","volume":"43","author":"Mehrabi","year":"2025","journal-title":"Contemp. Clin. Trials Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.sajb.2024.08.044","article-title":"The Anticancer Activity of Cuscuta campestris Yunck Extract: An Combined Study of In Vitro and In Vivo Experiments","volume":"174","author":"Selvi","year":"2024","journal-title":"South Afr. J. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"106658","DOI":"10.1016\/j.jff.2024.106658","article-title":"Integrating Network Analysis and Experimental Validation to Reveal the Mechanism of Cuscuta chinensis lam. Extract in the Treatment of IgA Nephropathy","volume":"124","author":"Zhang","year":"2025","journal-title":"J. Funct. Foods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100766","DOI":"10.1016\/j.hermed.2023.100766","article-title":"Holoparasites Cuscuta platyloba and Cuscuta xanthochortos Inhibit Staphylococcus aureus and Biofilm: A Metabolomics Approach","volume":"42","author":"Costa","year":"2023","journal-title":"J. Herb. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bedoui, A., Mufti, A., Feriani, A., Baccari, H., Bouallegue, A., Kharrat, M., Sobeh, M., Amri, M., and Abbes, Z. (2024). Unlocking the Hepatoprotective Potential of the Parasitic Plant Orobanche foetida Poir. Aqueous Extract against CCl4-Induced Liver Injury in Rat. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1320062"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1515\/reveh-2020-0048","article-title":"Toxicity of Carbon Tetrachloride, Free Radicals and Role of Antioxidants","volume":"36","author":"Unsal","year":"2021","journal-title":"Rev. Environ. Health"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"119485","DOI":"10.1016\/j.lfs.2021.119485","article-title":"The Protective Effect of Protocatechuic Acid on Hepatotoxicity Induced by Cisplatin in Mice","volume":"277","author":"Habib","year":"2021","journal-title":"Life Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"100693","DOI":"10.1016\/j.focha.2024.100693","article-title":"Quinic and Caffeic Acids Derivatives: Affecting Antioxidant Capacities and Phenolics Contents of Certain Therapeutic and Specialty Crops Employing Water and Ethanolic Extracts","volume":"4","author":"Islam","year":"2024","journal-title":"Food Chem. Adv."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"106554","DOI":"10.1016\/j.jff.2024.106554","article-title":"New Insights into the Hepato-Protective Effects of Ferulic Acid Based on Transcriptomic and Metabolomic Profiling","volume":"122","author":"Liu","year":"2024","journal-title":"J. Funct. Foods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102936","DOI":"10.1016\/j.tice.2025.102936","article-title":"Enhanced Hepatoprotective Efficacy of Quercetin Nanoparticles Versus Free Quercetin against Acrylamide-Induced Hepatotoxicity Through Modulation of MAPK\/NF-\u03baB\/NLRP3 Signaling Pathways and Molecular Docking Validation","volume":"95","author":"Atta","year":"2025","journal-title":"Tissue Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.sajb.2025.04.024","article-title":"Myricitrin, Kaempferol-3-O-Rutinoside, and Rutin from Acacia tortilis (Forssk.) Hayne ssp. raddiana Alleviate Liver Injury in Carbon Tetrachloride (CCl4)-Intoxicated Rats","volume":"181","author":"Amri","year":"2025","journal-title":"South Afr. J. Bot."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"\u017burawek, D., Pydyn, N., Major, P., Szade, K., Trzos, K., Ku\u015b, E., Po\u015bpiech, E., Ma\u0142czak, P., Radkowiak, D., and Budzy\u0144ski, A. (2025). Diosmetin Alleviates TNF\u03b1-Induced Liver Inflammation by Improving Liver Sinusoidal Endothelial Cell Dysfunction. Biomed. Pharmacother., 183.","DOI":"10.1016\/j.biopha.2025.117843"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"354","DOI":"10.5530\/ijper.57.2.45","article-title":"Insight into the Glycosylation Methods of the Flavonoids as an Approach to Enhance Its Bioavailability and Pharmacological Activities","volume":"57","author":"Dahiya","year":"2023","journal-title":"Ind. J. Pharm. Edu. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11651","DOI":"10.1080\/07391102.2023.2263889","article-title":"Antioxidative and ROS-Dependent Apoptotic Effects of Cuscuta reflexa Roxb. Stem against Human Lung Cancer: Network Pharmacology and In Vitro Experimental Validation","volume":"42","author":"Elasbali","year":"2024","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhang, L., Liu, C., Yin, L., Huang, C., and Fan, S. (2023). Mangiferin Relieves CCl4-Induced Liver Fibrosis in Mice. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-30582-3"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"100334","DOI":"10.1016\/j.metop.2024.100334","article-title":"Higher Levels of Plasmatic Saturated Fatty Acid Were Significantly Associated with Liver Fibrosis in HIV Mono-Infection: A Case-Control Study","volume":"24","author":"Araujo","year":"2024","journal-title":"Metab. Open"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ali, F.T., El-Gneady, F.F., Ahmed, F.A., Abd El-Sattar, N.E.A., Mostafa, M.M., El-Adl, K., and Elhady, M.M. (2025). Exploration of Phytochemical Components, Bioactivity Based Fractionation, and In Silico HMG-CoA Reductase Inhibitory Effect of Euphorbia Dendroides Chloroform Extract. Nat. Prod. Res., 1\u20139.","DOI":"10.1080\/14786419.2024.2448843"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"80910","DOI":"10.1007\/s11356-022-21422-4","article-title":"In Vivo Evaluation and Molecular Docking Studies of Schinus molle L. Fruit Extract Protective Effect against Isoproterenol-Induced Infarction in Rats","volume":"29","author":"Feriani","year":"2022","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"109429","DOI":"10.1016\/j.exer.2023.109429","article-title":"Mechanism for the Selective Uptake of Macular Carotenoids Mediated by the HDL Cholesterol Receptor SR-BI","volume":"229","author":"Li","year":"2023","journal-title":"Exp. Eye Res."},{"key":"ref_42","unstructured":"Komoda, T. (2010). Chapter 8\u2014The Scavenger Receptor Class B Type I: An HDL Receptor Involved in Lipid Transport and HDL Dependent Signaling. The HDL Handbook, Academic Press."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Widowati, W., Darsono, L., Utomo, H.S., Sabrina, A.H.N., Natariza, M.R., Valentinus Tarigan, A.C., Waluyo, N.W., Gleyriena, A.M., Siahaan, B.H., and Oktaviani, R. (2024). Antidiabetic and Hepatoprotection Effect of Butterfly Pea Flower (Clitoria Ternatea L.) through Antioxidant, Anti-Inflammatory, Lower LDH, ACP, AST, and ALT on Diabetes Mellitus and Dyslipidemia Rat. Heliyon, 10.","DOI":"10.1016\/j.heliyon.2024.e29812"},{"key":"ref_44","first-page":"730516","article-title":"Comparative Hepatoprotective Activity of Ethanolic Extracts of Cuscuta australis against Acetaminophen Intoxication in Wistar Rats","volume":"2014","author":"Folarin","year":"2014","journal-title":"Int. Sch. Res. Not."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1089\/jmf.2017.0139","article-title":"Cuscuta arvensis Beyr \u201cDodder\u201d: In Vivo Hepatoprotective Effects Against Acetaminophen-Induced Hepatotoxicity in Rats","volume":"21","author":"Yilmaz","year":"2018","journal-title":"J. Med. Food"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Peng, W.H., Chen, Y.W., Lee, M.S., Chang, W.T., Tsai, J.C., Lin, Y.C., and Lin, M.K. (2016). Hepatoprotective Effect of Cuscuta campestris Yunck. Whole Plant on Carbon Tetrachloride Induced Chronic Liver Injury in Mice. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122056"},{"key":"ref_47","first-page":"1225","article-title":"The genus Cuscuta (Convolvolaceac): An updated review on indigenous uses, phytochemistry, and pharmacology","volume":"22","author":"Noureen","year":"2019","journal-title":"Iran J. Basic Med. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Tej, A., Mekky, R.H., del Contreras, M.M., Feriani, A., Tir, M., L\u2019taief, B., Alshaharni, M.O., Faidi, B., Mnafgui, K., and Abbes, Z. (2024). Eucalyptus torquata Seeds: Investigation of Phytochemicals Profile via LC-MS and Its Potential Cardiopreventive Capacity in Rats. Food Biosci., 59.","DOI":"10.1016\/j.fbio.2024.103666"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"156053","DOI":"10.1016\/j.prp.2025.156053","article-title":"Combating Oxidative Stress in Non-Alcoholic Fatty Liver Disease: From Mechanisms to Therapeutic Strategies","volume":"272","author":"Mohammed","year":"2025","journal-title":"Pathol. Res. Pract."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"964","DOI":"10.2174\/1389200224666221118112009","article-title":"Pharmacokinetic\/Pharmacodynamic Study of Salt-Processed Product of Cuscutae Semen with Hepatoprotective Effects","volume":"23","author":"Zhang","year":"2022","journal-title":"Curr. Drug Metab."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Sato, A., Nakashima, H., Nakashima, M., Ikarashi, M., Nishiyama, K., Kinoshita, M., and Seki, S. (2014). Involvement of the TNF and FasL produced by CD11b Kupffer cells\/macrophages in CCl4-induced acute hepatic injury. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0092515"},{"key":"ref_52","first-page":"3835","article-title":"Role of macrophages in experimental liver injury and repair in mice (Review)","volume":"17","author":"Dong","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Iqbal, N., Zubair, H.M., Almutairi, M.H., Abbas, M., Akhtar, M.F., Aleya, L., Kamel, M., Saleem, A., Jabeen, Q., and Noreen, S. (2022). Hepatoprotective Effect of Cordia Rothii Extract against CCl4-Induced Oxidative Stress via Nrf2-NF\u03baB Pathways. Biomed. Pharmacother., 156.","DOI":"10.1016\/j.biopha.2022.113840"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wang, H., Li, Y., Bian, Y., Li, X., Wang, Y., Wu, K., Liu, C., Liu, Y., and Wang, X. (2022). Potential Hepatoprotective Effects of Cistanche Deserticola Y.C. Ma: Integrated Phytochemical Analysis Using UPLC-Q-TOF-MS\/MS, Target Network Analysis, and Experimental Assessment. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.1018572"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1007\/s10787-020-00700-y","article-title":"Zygophyllum album Leaves Extract Prevented Hepatic Fibrosis in Rats, by Reducing Liver Injury and Suppressing Oxidative Stress, Inflammation, Apoptosis and the TGF-\u03921\/Smads Signaling Pathways. Exploring of Bioactive Compounds Using HPLC-DAD-ESI-QTOF-MS\/MS","volume":"28","author":"Feriani","year":"2020","journal-title":"Inflammopharmacology"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Arjmand, A., Tsipouras, M.G., Tzallas, A.T., Forlano, R., Manousou, P., and Giannakeas, N. (2020). Quantification of Liver Fibrosis\u2014A Comparative Study. Appl. Sci., 10.","DOI":"10.3390\/app10020447"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"100205","DOI":"10.1016\/j.focha.2023.100205","article-title":"The Protective Role of Probiotics in the Mitigation of Carbon Tetrachloride (CCl4) Induced Hepatotoxicity","volume":"2","author":"Dua","year":"2023","journal-title":"Food Chem. Adv."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Duan, Y., Pan, X., Luo, J., Xiao, X., Li, J., Bestman, P.L., and Luo, M. (2022). Association of Inflammatory Cytokines with Non-Alcoholic Fatty Liver Disease. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.880298"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1002\/hep.30061","article-title":"Interleukin 2 Promotes Hepatic Regulatory T Cell Responses and Protects from Biliary Fibrosis in Murine Sclerosing Cholangitis","volume":"68","author":"Taylor","year":"2018","journal-title":"Hepatology"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Kwon, S.H., Lee, W.Y., Kim, Y.W., Ko, K.S., Bak, S.B., and Park, S.D. (2025). Isoquercitrin Attenuates Oxidative Liver Damage Through AMPK-YAP Signaling: An Integrative In Silico, In Vitro, and In Vivo Study. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062717"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Terao, J. (2023). Potential Role of Quercetin Glycosides as Anti-Atherosclerotic Food-Derived Factors for Human Health. Antioxidants, 12.","DOI":"10.3390\/antiox12020258"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"100603","DOI":"10.1016\/j.phyplu.2024.100603","article-title":"Comparing antioxidant and cytoprotective effects: Quercetin glycoside vs. aglycone from Ephedra alata","volume":"4","author":"Ullah","year":"2024","journal-title":"Phytomedicine Plus"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Najahi, A., Alaya, A., Mufti, A., Tir, M., del Contreras, M.M., Feriani, A., Harrath, A.H., Hfaiedh, N., and Tlili, N. (2024). HPLC-QTOF-MS Analysis of Polygonum maritimum Aerial Parts Extract and Focus on the Therapeutic Potential against Ethylene Glycol-Induced Lithiasis in Rats. Food Biosci., 57.","DOI":"10.1016\/j.fbio.2023.103481"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/S0021-9258(18)84277-6","article-title":"On tyrosine and tryptophane determinations in proteins","volume":"73","author":"Folin","year":"1927","journal-title":"J. Biol. Chem."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Mahdi, I., Fahsi, N., Annaz, H., Drissi, B., Barakate, M., Mahmoud, M.F., and Sobeh, M. (2023). Thymus satureioides Coss.: Mineral Composition, Nutritional Value, Phytochemical Profiling, and Dermatological Properties. Molecules, 28.","DOI":"10.3390\/molecules28124636"},{"key":"ref_66","first-page":"144","article-title":"Phytochemical analysis and total tannin content (TTC) of Delonix regia (Bojer ex. hook) Raf. bark by using different solvents collected from Saurashtra region","volume":"1","author":"Vala","year":"2022","journal-title":"Int. Assoc. Biol. Comput. Dig."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Bedoui, A., Feriani, A., Bouallegue, A., Baccari, H., Borgi, M.A., Kharrat, M., Sahri, N., Sobeh, M., Amri, M., and Abbes, Z. (2025). Unveiling the Cardiopreventive Effect of the Parasitic Weed Orobanche Crenata Forsk. Extract against Isoproterenol-Induced Myocardial Infarction in Rats. Food Biosci., 69.","DOI":"10.1016\/j.fbio.2025.106820"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Taamalli, A., Feriani, A., Lozano-Sanchez, J., Ghazouani, L., El Mufti, A., Allagui, M.S., Segura-Carretero, A., Mhamdi, R., and Arr\u00e1ez-Roman, D. (2020). Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage. Foods, 9.","DOI":"10.3390\/foods9060804"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/S0076-6879(78)52032-6","article-title":"Microsomal Lipid Peroxidation","volume":"52","author":"Buege","year":"1978","journal-title":"Methods Enzymol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1432-1033.1974.tb03714.x","article-title":"Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase","volume":"47","author":"Marklund","year":"1974","journal-title":"Eur. J. Biochem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in Vitro","volume":"105","author":"Aebi","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0076-6879(84)05015-1","article-title":"Assays of Glutathione Peroxidase","volume":"105","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.3390\/metabo12111031","article-title":"Euclea divinorum hiern: Chemical profiling of the leaf extract and its antioxidant activity in silico, in vitro and in Caenorhabditis elegans model","volume":"12","author":"Bogari","year":"2022","journal-title":"Metabolites"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/18\/10\/1524\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:13:39Z","timestamp":1760109219000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/18\/10\/1524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,10]]},"references-count":73,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["ph18101524"],"URL":"https:\/\/doi.org\/10.3390\/ph18101524","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,10]]}}}